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Preparation method and application of solid phase extraction column enriched with biogenic amines

A technology of solid phase extraction column and biological amine, which is applied in separation methods, chemical instruments and methods, solid adsorbent liquid separation, etc., can solve the problems of large amount of filler and sample processing, long processing time, etc., and achieves simple steps, Cost savings and guaranteed recovery

Inactive Publication Date: 2018-12-21
DALIAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the column tubes of these solid-phase extraction cartridges are all round tubes, with a large amount of filler and sample processing, and a long processing time.

Method used

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  • Preparation method and application of solid phase extraction column enriched with biogenic amines
  • Preparation method and application of solid phase extraction column enriched with biogenic amines
  • Preparation method and application of solid phase extraction column enriched with biogenic amines

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A preparation method of a solid phase extraction column enriched in biogenic amines includes the following steps:

[0033] S1. The column tube of the solid phase extraction column is a 1ml pipette tip; a polyethylene lower sieve with a particle size of 60μm and a diameter of 2.5mm is placed on the bottom of the pipette tip;

[0034] S2. Punch holes in the middle of the lid of the 1.5mL centrifuge tube and the bottom of the centrifuge tube. The pore size in the middle of the centrifuge tube lid is such that the top of the column tube of the solid phase extraction column can just pass through; the column tube passes through 1.5 from top to bottom. The aperture of the lid and bottom of the mL centrifuge tube is fixed in the centrifuge tube, and the distance between the tip of the pipette tip and the bottom of the centrifuge tube is 13mm;

[0035] S2. Put 40μl Thermo HyperSep Retain-CX solid phase extraction packing into the column tube described in step S1 to prepare a solid phas...

Embodiment 2

[0037] The solid phase extraction column made in Example 1 of the present invention was used to detect the biogenic amines in the mackerel samples.

[0038] The natural mackerel fish meat without any treatment is the test sample 1. To each gram of natural mackerel fish meat (ie the test sample 1) directly add 3 mg of the bio-amine standard solution to obtain the test sample 2; The test sample 1 and the test sample 2 are respectively subjected to the following steps for the enrichment of biogenic amine and the determination of biogenic amine content.

Embodiment 3

[0059] A preparation method of a solid phase extraction column enriched in biogenic amines includes the following steps:

[0060] S1. The column tube of the solid phase extraction column is a 1000μl pipette tip; a polyethylene lower sieve with a diameter of 60μm and a diameter of 2.5mm is placed on the bottom of the pipette tip;

[0061] S2. Punch holes in the middle of the lid of the 1.5mL centrifuge tube and the bottom of the centrifuge tube, and pass the column tube of the solid phase extraction column through the apertures of the lid and bottom of the 1.5mL centrifuge tube from top to bottom, and fix it in the centrifuge tube. The front end of the column tube is 13mm from the bottom of the centrifuge tube;

[0062] S3. Put 40 μl of Waters Oasis MCX solid phase extraction packing into the column tube described in step S1 to obtain a biologically enriched solid phase extraction column.

[0063] Using the solid phase extraction column prepared in this embodiment to enrich the biogeni...

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Abstract

The invention discloses a preparation method of a solid phase extraction column enriched with biogenic amines. The preparation method comprises the following steps that S1, a column pipe of the solidphase extraction column is a pipette superhead, and a lower sieve plate is placed at the bottom of the pipette superhead; S2, the middle portion and bottom of a centrifuge tube cover are drilled vertically, and the solid phase extraction column is fixed into a centrifugal tube after passing through the hole diameter of the centrifugal tube; and S3, the solid phase extraction column is obtained byplacing solid phase extraction packing into the column pipe. The invention further discloses an application method of the solid phase extraction column enriched with the biogenic amines. The application method comprises the steps of pretreatment of samples to be detected, activation of the solid phase extraction column, sample loading, leaching, elution and eluant concentration and drying. The solid phase extraction column prepared by the preparation method compares with a general solid phase extraction column, the amount of packing, the amount of sample treatment and the consumption of an organic solvent are reduced, the time of sample pretreatment is shortened, the cost is saved, and the detection of the biological amines is more convenient and efficient.

Description

Technical field [0001] The invention relates to the field of food safety detection, in particular to a preparation method and application of a solid phase extraction column enriched in bio-amine. Background technique [0002] Biogenic amines are low-molecular-weight, nitrogen-containing organic compounds, which are widely present in protein-rich foods and fermented products, such as fish, meat, cheese, beer, wine, etc. When the human body ingests excessive biogenic amines, it will cause headaches, nausea, palpitations and other toxic reactions, which may be life-threatening in severe cases; among them, histamine is the most toxic, followed by tyramine, and other biogenic amines can increase the toxicity of histamine. Therefore, the detection of biogenic amines in food has practical significance and is an important indicator to ensure food safety. [0003] Due to the complex composition of biological samples and serious matrix interference, the general sample pretreatment methods a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/02B01D15/22B01D15/20
CPCB01D15/206B01D15/22G01N30/02G01N30/06G01N2030/062
Inventor 纪超凡张婧博王帅赵铁峰李胜杰梁会朋林心萍
Owner DALIAN POLYTECHNIC UNIVERSITY
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