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Time resolution fluorescent immunochromatographic test strip for detecting pendimethalin, and preparation method and application thereof

A time-resolved fluorescence and immunochromatographic test paper technology, used in analytical materials, biological testing, fluorescence/phosphorescence, etc., can solve the problems of high professional requirements for operators, expensive detection costs, and complicated steps, and achieve a wide range of applications. , The effect of short detection time and simple operation

Active Publication Date: 2018-12-21
ZHENGZHOU TOBACCO RES INST OF CNTC +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the above methods require advanced detection instruments, the detection costs are expensive, the steps are cumbersome, time-consuming, and require high professionalism of operators, they are not suitable for high-throughput rapid screening detection in grassroots enterprises and institutions.

Method used

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  • Time resolution fluorescent immunochromatographic test strip for detecting pendimethalin, and preparation method and application thereof
  • Time resolution fluorescent immunochromatographic test strip for detecting pendimethalin, and preparation method and application thereof
  • Time resolution fluorescent immunochromatographic test strip for detecting pendimethalin, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Composition of the time-resolved fluorescence immunochromatography test strip for the detection of pendimethalin

[0042] A test strip

[0043] see figure 1 : The test strip is composed of a bottom plate, a sample absorption pad, a conjugate release pad, a nitrocellulose membrane and a water absorption pad;

[0044] The sample absorbent pad 1, the conjugate release pad 2, the nitrocellulose membrane 3 and the water absorbent pad 4 are sequentially overlapped and pasted on the bottom plate 7, and the conjugate release pad has 1 / 3 area covered by the sample absorbent pad from the starting end. , the end of the conjugate release pad is connected to the beginning of the nitrocellulose membrane, the end of the nitrocellulose membrane is connected to the beginning of the absorbent pad, the beginning of the sample absorbent pad is aligned with the beginning of the PVC bottom plate, and the end of the absorbent pad is aligned with the end of the PVC bottom plate ali...

Embodiment 2

[0047] Example 2 Preparation of time-resolved fluorescence immunochromatographic test strips for pendimethalin detection

[0048] The preparation method of the time-resolved fluorescence immunochromatography test strip for detecting pendimethalin mainly includes the following steps:

[0049] 1) Preparation of conjugate release pad: use commercially available fluorescent microspheres to label pendimethalin monoclonal antibody, and dilute it with a specific buffer system, soak the conjugate release pad in the dilution buffer, and freeze it in vacuum Preparation after drying;

[0050] 2) Preparation of nitrocellulose membrane: The pendimethalin hapten-carrier protein conjugate was sprayed onto the detection area on the nitrocellulose membrane to make the detection area; the goat anti-mouse antibody was sprayed onto the nitrocellulose The quality control area on the membrane is made into a quality control area;

[0051] 3) Assembling and cutting: Paste the sample absorption pad,...

Embodiment 3

[0104] Example 3 Application of time-resolved fluorescence immunochromatography test strips for the detection of pendimethalin

[0105] 1. Pretreatment of tobacco samples

[0106] Weigh 1.0±0.05 g of crushed tobacco samples into a polystyrene centrifuge tube, add 5 mL of methanol, vortex for 3 min, centrifuge at 6000 rpm at room temperature (20-25 °C) for 5 min, pipette 25 μL of the supernatant with 475 μL of deionized water was mixed and tested.

[0107] 2. Test with test strips

[0108] Draw 100 μL of the sample solution to be tested and drop it vertically into the sample hole of the test paper card, start timing when the liquid flows, and react for 10 minutes; insert the test paper card into the carrier of the KFT-100A fluorescence detector, and select the test paper to be tested by touching the display screen Item, press the "Start Test" button, the fluorescence detector will automatically scan the test paper card, read or print the test results on the display screen of ...

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Abstract

The invention discloses a time resolution fluorescent immunochromatographic test strip for detecting pendimethalin, and a preparation method and application thereof. The time resolution fluorescent immunochromatographic test strip comprises a bottom plate, a sample absorbent pad, a combination absorbent pad, a nitrocellulose membrane and a water absorbent pad. A pendimethalin monoclonal antibody labelled by fluorescent microspheres is embedded into the combination absorbent pad, and a detecting area and a quality control area are fixed to the nitrocellulose membrane. A pendimethalin hapten-carrier protein conjugate is sprayed in the detecting area, and an anti-sheep and anti-mouse antibody is sprayed in the quality control area. A pendimethalin hapten is obtained in the modes that N-(1-ethyl propyl ether)-3,4-methyl toluidine and 4-bromo-butyric acid ethyl ester react to generate hydrocarbylation N-(1-ethyl propyl ether)-3,4-methyl toluidine, then the hydrocarbylation N-(1-ethyl propylether)-3,4-methyl toluidine reacts with concentrated nitric acid to generate nitro hydrocarbonylation N-(1-ethyl propyl ether)-3,4-methyl toluidine, and then the nitro hydrocarbonylation N-(1-ethyl propyl ether)-3,4-methyl toluidine reacts with KOH. The time resolution fluorescent immunochromatographic test strip and a detecting method provided by the invention have the advantages that operationis easy, sensitivity is high, the detecting speed is high, and the cost is low.

Description

technical field [0001] The invention belongs to the field of pesticide residue detection, in particular to a time-resolved fluorescence immunochromatography test strip for detecting pendimethalin in tobacco and tobacco products, and a preparation method and application thereof. Background technique [0002] Pendimethalin is a dinitroaniline herbicide, and its mechanism of action is to inhibit the division of meristem cells. Dicotyledonous plants absorb pendimethalin in hypocotyls, while monocotyledonous plants absorb pendimethalin in young shoots. Pendimethalin is suitable for corn, soybean, wheat, peanut, cotton and other dryland crops to control crabgrass, foxtail, purslane and other annual grass and broadleaf weeds. Tobacco is mainly used for chemical bud suppression and tobacco weed control. Pendimethalin is classified as a low toxicity compound according to the Chinese pesticide acute toxicity classification standard. Pendimethalin is classified as Class III accordin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/58G01N33/533G01N21/64
CPCG01N21/6408G01N33/533G01N33/558G01N33/582G01N33/585
Inventor 陈黎范子彦刘惠民唐纲岭崔华鹏樊美娟谢复炜张晓兵余晶晶王晓瑜
Owner ZHENGZHOU TOBACCO RES INST OF CNTC
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