Selenium-enriched blueberry probiotic product and preparation method thereof
A raspberry probiotic and product technology, which is applied in the field of preparation of selenium-enriched blueberry probiotic products, can solve the problems of blueberry peel and blueberry seeds, oxidative destruction of water-soluble pigments, low utilization rate of blueberries, etc. safe effect
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Embodiment 1
[0033] Prepare 100L of yeast medium, add 300g of sodium selenite and 400g of zinc oxide powder, inoculate 10L of baker’s yeast after high-temperature sterilization, aerobically ferment at 28°C for 3 days, and stand at 10°C for 1 hour to obtain clear liquid and 2.25kg of yeast Add 10L water to the yeast cells, then add 2000U neutral protease and 350g calcium chloride, keep warm at 35°C for 4h, then add 10kg of crushed blueberry fruit powder, stir for 50min, filter to obtain filtrate Ⅰ and pomace Ⅰ; After slag I was sterilized by high temperature, clear liquid (all) was added, fermented aerobically at 28°C for 36 hours, and filtered to obtain filtrate II; Mix and stir Ⅰ at 13°C for 3 hours, then add filtrate Ⅱ, mix well and store at 4°C.
Embodiment 2
[0035] Prepare 100L of yeast medium, add 350g of sodium selenite and 500g of zinc oxide powder, inoculate 10L of baker’s yeast after high-temperature sterilization, aerobically ferment at 28°C for 4 days, and stand at 10°C for 1.5h to obtain clear liquid and 2.41kg of yeast Thalline: add 10L water to the yeast thalline, then add 2500U neutral protease and 400g calcium chloride, keep warm at 40°C for 3h, then add 12kg of crushed blueberry fruit powder, stir for 40min, filter to obtain filtrate I and pomace I; Pomace Ⅰ was sterilized by high temperature and added clear liquid (all), 25-30 ℃ aerobic fermentation for 48 hours, filtered to obtain filtrate Ⅱ; after 130L of milk was sterilized by high temperature, 15L of lactic acid bacteria and 16L of bifidobacteria were added to ferment for 48h, Then mix and stir with filtrate Ⅰ at 12°C for 5 hours, then add filtrate Ⅱ, mix well and store at 4°C. Comparative Example 1
Embodiment 1
[0036] Prepare 100L of yeast medium, add 300g of sodium selenite and 400g of zinc oxide powder, inoculate 10L of baker’s yeast after high-temperature sterilization, aerobically ferment at 28°C for 3 days, add 10L of water and 10kg of crushed blueberry fruit powder, stir for 50min, and then Add 2000U of neutral protease and 350g of calcium chloride, keep warm at 35°C for 5 hours, and filter to obtain filtrate Ⅰ; after high-temperature sterilization of 100L of milk, add 10L of lactic acid bacteria and 10L of bifidobacteria to ferment for 36 hours, and then mix with filtrate Ⅰ at 13°C Stir for 3 hours, mix well and store at 4°C.
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