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Microbial detection system and manufacturing method thereof

A technology for microbial detection and dry powder culture medium, which is applied in biochemical equipment and methods, microbial measurement/inspection, enzymology/microbiology devices, etc., can solve the problem of low results, and achieve accurate results, convenient use and simple operation. Effect

Pending Publication Date: 2018-12-28
孙百莉
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The quick test paper method directly sucks 1mL of the sample liquid and drops it on the paper without diluting the sample liquid. Therefore, when testing samples with bactericidal and antibacterial effects and high osmotic pressure samples, the results are low, and the quick test paper is not good. It is suitable for the detection of such samples; the finished separation plate and the finished fermentation tube are the separation plate and fermentation tube prepared by the manufacturer, so there are the same disadvantages as the conventional method

Method used

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  • Microbial detection system and manufacturing method thereof
  • Microbial detection system and manufacturing method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1, the preparation and application of the microorganism detection system used for the detection of the total number of colonies

[0034] 1. Preparation of Microbial Detection System

[0035] 1. Preparation of culture medium

[0036] Tryptone 5.0g / L, yeast extract 2.5g / L, glucose 1.0g / L, sodium polyacrylate 40.0g / L, mix well;

[0037] 2. Packing

[0038] 0.485g / bag divided and sealed;

[0039] 3. Sterilization

[0040] The subpackaged modified dry powder medium in bags was sterilized by γ-rays for use, and the sterile container was sterilized at 121° C. for 15 minutes before use.

[0041] 2. The microbial detection system detects the total number of microbial colonies

[0042] 1. Inoculation

[0043] Take 10 samples respectively, and take 25 g of each sample into a sterile homogenizing bag according to aseptic operation, add 225 mL of sterile physiological saline, and homogenize with a slap-type homogenizer for 1 min to make 10 samples. -1 1 mL of the s...

Embodiment 2

[0055] Example 2. Preparation and application of a microorganism detection system for the detection of water coliforms

[0056] 1. Preparation of Microbial Detection System

[0057] 1. Preparation of culture medium

[0058] Peptone 10.0g / L, beef extract 3.0g / L, lactose 5.0g / L, sodium chloride 5.0g / L, bromocresol violet 0.016g / L, sodium polyacrylate 40.0g / L, mix well;

[0059] 2. Packing

[0060] 6.3g / bag divided and sealed;

[0061] 3. Sterilization

[0062] The subpackaged modified dry powder medium in bags was sterilized by γ-rays for use, and the sterile container was sterilized at 121° C. for 15 minutes before use.

[0063] 2. Microbial detection system to detect water coliforms

[0064] 1. Inoculation

[0065] Tear open the bag of modified dry powder medium aseptically, pour it into a sterile container, place the sterile container on the surface of the water platform, pour 100 mL of water sample into the sterile container, mix it along the table, and let it stand fo...

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Abstract

The invention discloses a microbial detection system and a manufacturing method thereof. The microbiological detection system comprises a bagged modified dry powder medium and a sterile container. Cold water soluble gel is used as a coagulant, ray sterilization is used, and heat-labile components can be added together to the medium, so the sterilization and filtration sterilization steps are omitted, and the system is convenient to use; when pathogenic bacteria are separated, the detection system can complete the detection of a dilution; the hardness of the gel is moderate, and the bacteria can be chosen for identification; in the water coliform detection, a 100 mL water sample can be detected at a time, and the operation is simple; and the gel can intercept the gas generated by coliform fermented lactose, so the result is accurate.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the field of rapid detection of microorganisms, and in particular to a system for detection of microorganisms and a preparation method thereof. Background technique [0002] At present, the national standard method is mainly used for the detection of food microorganisms, the plate counting method and the multi-tube fermentation method are used for the detection of health indicator bacteria, and the plate separation method is used for the detection of pathogenic bacteria. The three methods appealed all require the examiner to do a lot of preparation work, including brushing, preparation of culture medium, sterilization, and disposal of waste. The medium used in the plate counting method needs to be autoclaved, cooled to 45°C and poured into the plate, and the operator needs to strictly control the temperature. If the temperature is too high, heat-labile microorganisms and damaged micr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/34C12Q1/10C12Q1/04
CPCC12M41/36C12Q1/04C12Q1/10
Inventor 孙百莉
Owner 孙百莉
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