Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for preparing major yolk protein from coelomic fluid of sea cucumber

A technology for egg yolk protein and coelom fluid, which is applied in the field of preparing main egg yolk protein and protein preparation, can solve problems such as pollution, waste of resources and environment, and achieve the effects of reducing pollution, improving utilization rate and simple method.

Active Publication Date: 2019-01-01
DALIAN POLYTECHNIC UNIVERSITY
View PDF5 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Sea cucumber body cavity fluid is a by-product of the sea cucumber processing process, which causes waste of resources and environmental pollution after being discarded as waste
Sea cucumber is rich in nutrition and has high economic value. Its body cavity fluid contains a variety of active ingredients, but it has not been well utilized. At present, the research on sea cucumber body cavity fluid is limited to the research on immune components. Sea cucumber body cavity fluid is also rich in The main yolk protein in the sea cucumber has high nutritional value and is rich in essential amino acids. If the main yolk protein in the coelom fluid of sea cucumbers can be recycled, it will be of great significance to the high-value utilization of sea cucumbers

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing major yolk protein from coelomic fluid of sea cucumber

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] S1. Pretreatment of sea cucumber body cavity fluid: Use filter paper to absorb the liquid on the surface of fresh imitation sea cucumber, cut the sea cucumber from the abdomen, collect the body cavity fluid, centrifuge at 4℃, 1500×g for 15 minutes, collect the supernatant, and obtain the body cavity Clear liquid

[0035] S2, dialysis method for low-temperature desalination: using a 2KDa dialysis membrane and 10mM pH7 phosphate buffer solution to dialyze the body cavity clear fluid obtained in step S1 for 48 hours at 4°C, and the dosage of the phosphate buffer is 10 times that of the body cavity clear fluid;

[0036] S3. Extract the main egg yolk protein: Add polyethylene glycol with a molecular weight of 6000 to the sea cucumber body cavity clear liquid obtained in step S2 at 4°C to make the final concentration of polyethylene glycol 10% (weight), and use 0.5 mol / L NaOH solution or 0.5mol / L hydrochloric acid solution to adjust the pH of the reaction system to 7.0, let it st...

Embodiment 2

[0043] S1. Pretreatment of sea cucumber body cavity fluid: Use filter paper to absorb the liquid on the surface of fresh imitation sea cucumber, cut the sea cucumber from the abdomen, collect the body cavity fluid, centrifuge at 4°C and 2000×g for 10 minutes, collect the supernatant, and obtain the body cavity Clear liquid

[0044] S2. Dialysis method for low-temperature desalination: Use a 3.5KDa dialysis membrane and a 20mM pH 7.4 phosphate buffer solution to dialyze the body cavity clear fluid obtained in step S1 for 60 hours at 4°C. The amount of the phosphate buffer is the amount of the body cavity clear fluid 15 times;

[0045] S3. Extract the main egg yolk protein: Add polyethylene glycol with a molecular weight of 6000 to the sea cucumber body cavity clear liquid obtained in step S2 at 4°C to make the final concentration of polyethylene glycol 12% (by weight), using 0.5 mol / L NaOH solution or 0.5mol / L hydrochloric acid solution to adjust the pH of the reaction system to 7...

Embodiment 3

[0052] S1. Pretreatment of sea cucumber body cavity fluid: Use filter paper to absorb the liquid on the surface of fresh imitation sea cucumber, cut the sea cucumber from the abdomen, collect the body cavity fluid, centrifuge at 4℃, 1800×g for 12 min, collect the supernatant, and obtain the body cavity Clear liquid

[0053] S2. Dialysis method for low-temperature desalination: Use a 4KDa dialysis membrane and a 25mM pH8 phosphate buffer solution to dialyze the body cavity clear fluid obtained in step S1 for 72 hours. The amount of the phosphate buffer is 20 times that of the body cavity clear fluid. The temperature of the phosphate buffer solution in the whole desalting process is 4℃;

[0054] S3. Extract the main egg yolk protein: Add polyethylene glycol with a molecular weight of 6000 to the sea cucumber body cavity clear liquid obtained in step S2 at 4°C to make the final concentration of polyethylene glycol 14% (weight), using 0.5 mol / L NaOH solution or 0.5mol / L hydrochloric ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for preparing major yolk protein from a coelomic fluid of sea cucumber. The specific processes comprise the steps of pretreatment of the coelomic fluid of the sea cucumber, low-temperature desalting by a dialysis method, extraction of the major yolk protein, redissolving, dialysis and drying. The by-product, namely the coelomic fluid of the sea cucumber, generatedduring processing of the sea cucumber is used as a raw material, so that the utilization rate of the by-product generated during processing of the sea cucumber is raised, the protein resource of the coelomic fluid of the sea cucumber is fully developed and utilized, meanwhile, the pollution of the by-product of the sea cucumber to the environment is reduced, and the method has great significance to development and utilization of the major yolk protein in the coelomic fluid of the sea cucumber. Compared with traditional protein precipitation methods, which are hard to achieve scale production,such as ammonium sulfate salting-out, ethanol precipitation and isoelectric precipitation, the method provided by the invention is mild in operation condition, and therefore denaturation of biomacromolecules is not liable to occur; the extraction rate is up to 70%; and the method provided by the invention is simple, meanwhile, a large number of samples can be treated at a time, assistance of complex instruments is not needed, and the method is suitable for large-scale production.

Description

Technical field [0001] The invention belongs to the technical field of food processing, and relates to a protein preparation method, and more specifically, to a method for preparing main egg yolk protein from sea cucumber body cavity fluid. Background technique [0002] Sea cucumber body cavity fluid is a by-product of the sea cucumber construction process, which causes waste of resources and environmental pollution after being discarded as waste. Sea cucumbers are rich in nutrients and have high economic value. Their body cavity fluid contains a variety of active ingredients, but they are not well used. The current research on sea cucumber body cavity fluids is limited to the research on immune components. Sea cucumber body cavity fluid is also rich in The main egg yolk protein, the main egg yolk protein has high nutritional value and is rich in essential amino acids. If the main egg yolk protein in the body cavity fluid of the sea cucumber can be recycled, it will be of great s...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07K14/435C07K1/30C07K1/34C07K1/14
CPCC07K14/43504
Inventor 吴海涛李傲婷商文慧杜椅楠姜昕昱洪佳男
Owner DALIAN POLYTECHNIC UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com