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Hydrogenophaga sp. H7 capable of degrading aromatic compounds and removing nitrogen and arsenic, and application thereof

A technology of phagocytosis and ability, applied in the field of hydrogen phagocytosis H7, can solve the problems of long repair period, unsuitable water pollution treatment, secondary pollution, etc.

Inactive Publication Date: 2019-01-01
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are few reports on remediation methods for compound pollution of arsenic and aromatic compounds, mainly including elution extraction and phytoremediation
The former is easy to cause secondary pollution and lead to a decline in soil fertility; the latter is easily restricted by soil type and climate, and has a long repair period and is not suitable for water pollution treatment, and mainly focuses on the removal of arsenic pollution.
From the current reports, it can be seen that the single pollution of arsenic and aromatic compounds can be treated by microorganism-based methods, and the microbial treatment method has its unique advantages. Microorganisms of compounds are hardly reported

Method used

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  • Hydrogenophaga sp. H7 capable of degrading aromatic compounds and removing nitrogen and arsenic, and application thereof
  • Hydrogenophaga sp. H7 capable of degrading aromatic compounds and removing nitrogen and arsenic, and application thereof
  • Hydrogenophaga sp. H7 capable of degrading aromatic compounds and removing nitrogen and arsenic, and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1: Hydrophage H7 was isolated from the soil of Dayejin Mine, Hubei

[0025] (1) Sample collection: The surface soil of a copper-iron mine in Daye City, Hubei Province, China was collected in May 2010.

[0026] (2) Sample enrichment: Accurately weigh 100g of soil sample into a 250mL sterilized Erlenmeyer flask, add 5mL of 80mM sodium arsenite NaAsO 2 (As(III)), stir gently and place in an incubator at 28°C for one week, pay attention to add sterile water to ensure that the sample is not dry.

[0027] (3) Isolation of arsenic-resistant bacteria: Accurately weigh 10 g of As(III)-enriched soil sample into a Erlenmeyer flask filled with 90 mL of sterile normal saline, shake it in a shaker at 28°C for half an hour, and then take 1 mL to 9 mL sequentially Dilute stepwise to 10 in sterile saline -3 、10 -4 、10 -5 , respectively take 0.1mL coated with MMNH containing 400μM As(III) 4 For solid medium plates, 3 plates were coated for each dilution, and cultured in a 28...

Embodiment 2

[0034] Example 2: Arsenic Oxidation Curve, 3-Hydroxybenzoic Acid and 4-Hydroxybenzoic Acid Degradation Curve and Nitrate Reduction Curve of Hydrogenophage H7

[0035] Pick a single clone of Hydrophage H7 and inoculate it into 100 mL of R2A liquid medium, and culture it in a shaker at 28°C until OD 600 It is about 0.45, it is used as seed liquid, is inoculated in fresh 100mL 1 / 10ST liquid culture medium (initial OD 600 <0.01), and add 40 μL of 1M As(III) to the medium to make the final concentration to 400 μM. In addition, 250 μL of 100 g / L 3-hydroxybenzoic acid and 4-hydroxybenzoic acid were added to the medium at the same time to make the final concentration to 250 mg / L, and they were placed in a shaker at 28°C for shaking culture. Samples were taken every 4 hours. Take 1.0 mL each time to measure the concentration of trivalent arsenic As(III) and pentavalent arsenic As(V) and the concentration of 3-hydroxybenzoic acid and 4-hydroxybenzoic acid. The concentration of trival...

Embodiment 3

[0040] Example 3: Hydrophage H7 has the effect of oxidizing trivalent arsenic, the degradation effect of 3-hydroxybenzoic acid and 4-hydroxybenzoic acid and the effect of reducing chemical oxygen demand (COD) in simulated composite polluted water

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Abstract

The invention specifically relates to Hydrogenophaga sp. H7 capable of degrading aromatic compounds and removing nitrogen and arsenic, and application thereof, belonging to the technical field of application of environmental microorganisms. The Hydrogenophaga sp. H7 belongs to Hydrogenophaga sp. and is preserved in China Center for Type Culture Collection under the accession number CCTCC No. M2018149. The arsenic oxidizing bacterium Hydrogenophaga sp. H7 of the invention is an arsenic oxidizing bacterium capable of degrading various aromatic compounds such as 3-hydroxybenzoic acid, 4-hydroxybenzoic acid, benzoic acid and phenol, and the bacterium can oxidize As<3+> with high toxicity into As<5+> with low toxicity and degrade aromatic compounds such as 3-hydroxybenzoic acid and 4-hydroxybenzoic acid. The bacterium is also a denitrifying bacterium which has great potential in clearing pollution in composite contaminated areas and also has application value in removing nitrogen pollution.

Description

technical field [0001] The invention belongs to the technical field of environmental microorganism application, and specifically relates to hydrogen phagocytosis H7 strains capable of degrading aromatic compounds, removing nitrogen and arsenic, and its use. The strain can degrade aromatic compounds such as 3-hydroxybenzoic acid and 4-hydroxybenzoic acid, can oxidize highly toxic trivalent arsenic to low-toxic pentavalent arsenic, and can reduce nitrate to nitrogen. H7 can purify compound pollution of arsenic and aromatic compounds and purify nitrogen pollution. Background technique [0002] At present, many polluted areas are not a single type of pollution, but complex pollution of heavy metals and organic pollutants. As typical representatives of heavy metals and organic pollutants, arsenic and aromatic compounds are often found together. Arsenic (As) is a highly toxic metalloid, which is extremely harmful to the human body and the environment, and many aromatic compounds...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34B09C1/10C12R1/01C02F101/34C02F101/20C02F101/16
CPCB09C1/10C02F3/34C02F2101/163C02F2101/20C02F2101/34C02F2101/345C12N1/205C12R2001/01
Inventor 王革娇范霞王倩
Owner HUAZHONG AGRI UNIV
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