Preparation method and application of plasma treated origanum majorana extract

A plasma and marjoram technology, applied in the field of preparation of marjoram extract, can solve the problems of low extraction efficiency, weak safety, waste of resources, etc., and achieves high extraction efficiency, short processing time, and reduced waste of resources. Effect

Inactive Publication Date: 2019-01-04
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the technical defects existing in the prior art, such as: low extraction efficiency

Method used

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  • Preparation method and application of plasma treated origanum majorana extract
  • Preparation method and application of plasma treated origanum majorana extract
  • Preparation method and application of plasma treated origanum majorana extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Experimental Materials:

[0028]

[0029] (1) Preparation of marjoram ethanol extract:

[0030] Weigh 10g of marjoram leaves, clean them, cut them, dry them, soak them in 40mL of absolute ethanol, wherein the mass volume ratio of marjoram leaves to absolute ethanol is 2:8, and place them in a water bath shaker at 25°C Shake and extract for 24 hours to obtain marjoram ethanol extract.

[0031] (2) Preparation of Marjoram extract:

[0032] Put the marjoram ethanol extract in a pulsed magnetic field apparatus at a temperature of 4°C, a pulse intensity of 1.0T, and a pulse number of 10; then filter the marjoram ethanol extract, and place the filtrate in a rotary evaporator Concentrate to obtain marjoram extract with an extraction rate of 61.38%.

[0033] (3) Plasma treatment of marjoram extract:

[0034] The Marjoram extract is placed in a small beaker, and then the Marjoram extract is placed in a plasma spectrometer for processing. Nitrogen is the protective gas, th...

Embodiment 2

[0037] (1) Preparation of marjoram ethanol extract:

[0038] Weigh 10g of marjoram leaves, clean them, cut them, dry them, soak them in 40mL of absolute ethanol, wherein the mass volume ratio of marjoram leaves to absolute ethanol is 2:8, and place them in a water bath shaker at 25°C Shake and extract for 24 hours to obtain marjoram ethanol extract.

[0039] (2) Preparation of Marjoram extract:

[0040] Put the ethanol extract of marjoram in a pulsed magnetic field apparatus, the temperature is 12°C, the pulse intensity is 2.0T, and the number of pulses is 30; then the ethanol extract of marjoram is filtered, and the filtrate is placed in a rotary evaporator Concentrate to obtain marjoram extract with an extraction rate of 75.26%.

[0041] (3) Plasma treatment of marjoram extract:

[0042] The Marjoram extract is placed in a small beaker, then the Marjoram extract is placed in a plasma spectrometer for processing, nitrogen is the protective gas, the nitrogen flow rate is 10...

Embodiment 3

[0045] (1) Preparation of marjoram ethanol extract:

[0046] Weigh 10g of marjoram leaves, clean them, cut them, dry them, soak them in 40mL of absolute ethanol, wherein the mass volume ratio of marjoram leaves to absolute ethanol is 2:8, and place them in a water bath shaker at 25°C Shake and extract for 24 hours to obtain marjoram ethanol extract.

[0047] (2) Preparation of Marjoram extract:

[0048] Put the ethanol extract of marjoram in a pulsed magnetic field apparatus at a temperature of 25°C, a pulse intensity of 4.0T, and a pulse number of 40; then filter the ethanol extract of marjoram, and place the filtrate in a rotary evaporator Concentrate to obtain marjoram extract with an extraction rate of 96.61%.

[0049] (3) Plasma treatment of marjoram extract:

[0050] Put the marjoram extract in a small beaker, then place the marjoram extract in a plasma spectrometer, and treat it with air as a protective gas. The plasma power is 600W, and the processing time is 6min to ...

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Abstract

The invention belongs to the field of antibacterial agents and food additives, and particularly relates to a preparation method and application of a plasma treated origanum majorana extract. The preparation method comprises the following specific steps: thoroughly cleaning leaves of origanum majorana, shearing and drying the leaves of the origanum majorana, adding anhydrous ethanol and soaking theleaves of the origanum majorana, and shaking out and extracting to obtain an origanum majorana ethanol extract liquid; placing the origanum majorana ethanol extract liquid in a pulsed magnet field and carrying out pulse magnet field treatment, and filtering and concentrating to obtain an origanum majorana extract; and placing the origanum majorana extract in a plasma spectrometer and treating theoriganum majorana extract to obtain the plasma treated origanum majorana extract. The origanum majorana extract is prepared by a pulsed magnet field technology, the extracting efficiency is high, andresource waste is reduced effectively; the origanum majorana extract is treated by plasma, growth of food-borne pathogens and hospital-acquired-infection pathogenic bacteria can be inhibited effectively, bacterial food poisoning is prevented effectively, food safety is ensured, the shelf life of food is prolonged, and application of origanum majorana in food is expanded.

Description

technical field [0001] The invention belongs to the field of antibacterial agents and food additives, and in particular relates to a preparation method and application of a plasma-treated marjoram extract. Background technique [0002] Marjoram (Origanum majorana), also known as Mayu grass, belongs to the genus Origanum of the Lamiaceae. It is native to the Mediterranean region and distributed in Turkey, Egypt and other places. Marjoram is warm in nature and slightly bitter in taste. It has the effects of invigorating qi and refreshing, expelling toxins and invigorating the spleen, relieving cough and asthma, helping digestion, reducing flatulence, and relieving spasm. In addition, marjoram is aromatic, with sweet pine and citrus aromas, and is a commonly used spice. Stem and leaf all contain volatile oil, main component is thymol (Thymol) and carvacrol (Carvacrol). At present, the commonly used marjoram extraction methods are mainly water extraction and alcohol extraction...

Claims

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Application Information

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IPC IPC(8): A23B4/20A23B7/154
CPCA23B4/20A23B7/154A23V2002/00A23V2250/21
Inventor 林琳顾玉磊崔海英
Owner JIANGSU UNIV
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