Biodegradation method for DON (deoxynivalenol) in corn germ meal

Abstract

The invention discloses a biodegradation method for DON (deoxynivalenol) in corn germ meal. A compound koji starter is formed by mixing 15%-25% of one or two of lactobacillus acidophilus koji starterand saccharomyces cerevisiae koji starter, 45%-65% of one or two of aspergillus niger koji starter and aspergillus oryzae koji starter and 20%-30% of bacillus subtilis koji starter. The spouting corngerm meal is inoculated with the compound koji starter in the inoculum size of 3%-5%, stirring the performed for 30 min, 3.0%-4.2% of an ammonium sulfate aqueous solution is added until the moisture content of the material is 42%-48%, the material is uniformly stirred and left to stand for fermentation for 4-20 h, after the temperature is increased 36-38 DEG C in 30 cm of the material layer, turning and throwing (aeration) is performed to decrease the temperature to 27-30 DEG C, and turning and throwing are stopped. When the temperature is increased to 36-38 DEG C again in 30 cm of the material layer, turning and throwing (aeration) is performed to decrease the temperature to 27-30 DEG C, and turning and throwing are stopped. The operation is performed repeatedly and the whole process is kept for 36-48 h. after the material temperature is not increased quickly, the fermented material is turned and thrown to be cooled to 26-28 DEG C, the material is flattened, compacted and then sealedfor 20-30 h, and fermentation degradation is ended when sour fragrance spills a fermentation tank.

Description

technical field [0001] The invention relates to the technical field of biodegradation of mycotoxins polluted by grain and oil, in particular to the biodegradation technology of vomitoxin polluted by spraying corn germ meal, a by-product of corn deep processing. technical background [0002] According to the estimates of the Food and Agriculture Organization of the United Nations, the losses caused by mycotoxin pollution in the world amount to hundreds of millions of dollars every year, and the harm to humans and animals is even more difficult to count. In recent years, the negative impact of mycotoxin pollution on livestock production has gradually been recognized, and foreign scientific research institutions have begun to carry out a series of studies on the biodegradation of mycotoxins. In China, there are few research reports on DON-degrading bacteria and detoxification enzymes. Screening strains capable of producing DON-degrading enzymes and obtaining highly expressed de...

AI Technical Summary

Problems solved by technology

But so far, there is no research report on the deoxygenation enzyme of vomitoxin, and there is no related report on the development of feed additives using microorganisms or the enzymes produced by them