Biodegradation method for DON (deoxynivalenol) in corn germ meal

A production method and microbial fermentation technology, which is applied in the biodegradation field of deoxynivalenol contaminated by sprayed corn germ meal, a by-product of corn deep processing, can solve the problems of research and development of feed additives, and the lack of research reports on deoxynivalenol-degrading enzymes.

Inactive Publication Date: 2019-01-11
SHANGHAI HONGYUAN BIOTECH
View PDF5 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, there is no research report on the deoxygenation enzyme of vomitoxin, and there is no rel

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0012] 1. Utilize the production method of Lactobacillus acidophilus fermentation material, the production method of Bacillus subtilis fermentation material, the production method of Saccharomyces cerevisiae fermentation material and the black The production method of Aspergillus fermentation material, the production method of Aspergillus oryzae fermentation material, and the "self-supporting keyboard" disclosed by my patent No. ZL2012 20624896.7, making Lactobacillus acidophilus koji, Bacillus subtilis koji, Saccharomyces cerevisiae koji, black Aspergillus koji and Aspergillus oryzae koji are used for later use.

[0013] The making fermented material of said ZL 201010148491.6 comprises the following steps:

[0014] (1) Making Lactobacillus acidophilus fermentation material:

[0015] A. Production of Lactobacillus acidophilus fermentation medium

[0016] The following raw materials are taken in proportion by weight: 15-25 parts of corn flour, 40-50 parts of wheat bran, 8-20 ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Thicknessaaaaaaaaaa
Login to view more

Abstract

The invention discloses a biodegradation method for DON (deoxynivalenol) in corn germ meal. A compound koji starter is formed by mixing 15%-25% of one or two of lactobacillus acidophilus koji starterand saccharomyces cerevisiae koji starter, 45%-65% of one or two of aspergillus niger koji starter and aspergillus oryzae koji starter and 20%-30% of bacillus subtilis koji starter. The spouting corngerm meal is inoculated with the compound koji starter in the inoculum size of 3%-5%, stirring the performed for 30 min, 3.0%-4.2% of an ammonium sulfate aqueous solution is added until the moisture content of the material is 42%-48%, the material is uniformly stirred and left to stand for fermentation for 4-20 h, after the temperature is increased 36-38 DEG C in 30 cm of the material layer, turning and throwing (aeration) is performed to decrease the temperature to 27-30 DEG C, and turning and throwing are stopped. When the temperature is increased to 36-38 DEG C again in 30 cm of the material layer, turning and throwing (aeration) is performed to decrease the temperature to 27-30 DEG C, and turning and throwing are stopped. The operation is performed repeatedly and the whole process is kept for 36-48 h. after the material temperature is not increased quickly, the fermented material is turned and thrown to be cooled to 26-28 DEG C, the material is flattened, compacted and then sealedfor 20-30 h, and fermentation degradation is ended when sour fragrance spills a fermentation tank.

Description

technical field [0001] The invention relates to the technical field of biodegradation of mycotoxins polluted by grain and oil, in particular to the biodegradation technology of vomitoxin polluted by spraying corn germ meal, a by-product of corn deep processing. technical background [0002] According to the estimates of the Food and Agriculture Organization of the United Nations, the losses caused by mycotoxin pollution in the world amount to hundreds of millions of dollars every year, and the harm to humans and animals is even more difficult to count. In recent years, the negative impact of mycotoxin pollution on livestock production has gradually been recognized, and foreign scientific research institutions have begun to carry out a series of studies on the biodegradation of mycotoxins. In China, there are few research reports on DON-degrading bacteria and detoxification enzymes. Screening strains capable of producing DON-degrading enzymes and obtaining highly expressed de...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A23L5/20A23K10/37
CPCA23K10/37A23L5/28Y02P60/87
Inventor 王德昌张玉国张楠楠
Owner SHANGHAI HONGYUAN BIOTECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap