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Micro-fluidic chip with chromatographic sheet serving as substrate and production method of micro-fluidic chip

A technology of thin-layer chromatography plate and microfluidic chip, which is applied in the field of microfluidic chip and its production, can solve the problems of lack of thin-layer chromatography separation ability, expensive processing equipment, high environmental requirements, etc., and achieve rapid batch replication, improve Analytical efficiency and accuracy, effect of simplified sample preparation steps

Pending Publication Date: 2019-01-11
汉江师范学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The invention provides a microfluidic chip based on a thin-layer chromatographic plate and a manufacturing method thereof to solve the problem that the fluid channels on the traditional material microfluidic chip do not have the separation ability of thin-layer chromatography; the manufacturing method of the present invention uses The equipment is simple, does not require a special clean environment, and can be copied in batches quickly. It can solve the bottleneck problems that are difficult to popularize, such as expensive traditional microfluidic chip processing equipment, high environmental requirements, and complicated processes.

Method used

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  • Micro-fluidic chip with chromatographic sheet serving as substrate and production method of micro-fluidic chip
  • Micro-fluidic chip with chromatographic sheet serving as substrate and production method of micro-fluidic chip
  • Micro-fluidic chip with chromatographic sheet serving as substrate and production method of micro-fluidic chip

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preparation example Construction

[0038] see Figure 1 ~ Figure 4 , the preparation method of the microfluidic chip comprises the following steps:

[0039] (1) Obtain TLC plate 1, wax film 2, and transparent platen 3;

[0040] (2) if figure 1 and figure 2 , the waxy film 2 is flattened and covered on the surface of the stationary phase layer of the thin-layer chromatography plate 1, the transparent press plate 3 is covered on the surface of the waxy film 2, and the transparent press plate 3, the waxy film 2, and the thin layer are surrounded by clips 4 The chromatographic plate 1 is clamped in three layers. Wrinkles and arches should not appear in the middle waxy film 2;

[0041] (3) if image 3 , placing the three layers clamped in step (2) horizontally under the laser head 6 of the laser engraving machine 5, the transparent pressure plate 3 is located on the uppermost layer, and the laser beam 7 can be perpendicular to the transparent pressure plate 3;

[0042] (4) Start the laser engraving machine 5,...

Embodiment 1

[0050] A method for manufacturing a microfluidic chip based on a silica gel GF254 glass-based thin-layer chromatography plate, the specific steps are:

[0051] (1) A TLC plate with silica gel GF254 as the stationary phase and glass as the support plate was selected as the substrate, and the coating thickness of the stationary phase was 200 μm; wax paper was selected as the wax film; a 0.5 mm thick quartz sheet was selected as the transparent pressing plate , the surface of the quartz sheet is smooth and clean, without defects such as sand, bubbles, and wrinkles visible to the naked eye;

[0052] (2) Flatten the typing stencil on the surface of the stationary phase layer of the TLC plate, cover the surface of the quartz sheet on the typing stencil, and clamp the quartz sheet, typing stencil, and TLC plate with clamps around the three layers, and the middle Layers of typing stencil should not appear wrinkled and arched;

[0053] (3) Place the three layers clamped in step (2) ho...

Embodiment 2

[0057] A method for manufacturing a microfluidic chip based on microcrystalline cellulose F254 aluminum-based thin-layer chromatography plate, the specific steps are:

[0058] (1) A thin-layer chromatographic plate with microcrystalline cellulose F254 as the stationary phase and an aluminum plate as the support plate was selected as the substrate, and the coating thickness of the stationary phase was 100 μm; carbon paper was selected as the wax film; a 0.5 mm thick quartz sheet was selected as the Transparent press plate, the surface of the quartz sheet is smooth and clean, without defects such as sand, bubbles, and wrinkles visible to the naked eye;

[0059] (2) Flatten the carbon paper to cover the surface of the stationary phase layer of the TLC plate, cover the surface of the carbon paper with a quartz plate, and clamp the quartz plate, carbon paper, and TLC plate with clamps around the three layers, and the middle layer of the carbon paper does not Wrinkling and arching s...

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Abstract

The invention discloses a micro-fluidic chip with a chromatographic sheet serving as a substrate and a production method of the micro-fluidic chip. The micro-fluidic chip comprises a chromatographic sheet substrate and at least one micro-fluidic channel constructed on the surface of the substrate. The production method of the micro-fluidic chip comprises the steps: sequentially stacking and clamping three layers including a chromatographic sheet, a waxy thin film and a transparent pressing plate, placing the three layers below a laser head of a laser engraving machine, and scanning a designedmicro-fluidic channel pattern by using a laser beam; melting a hydrophobic waxy material on the waxy thin film by using the laser beam, and transferring the hydrophobic waxy material to a stationary phase layer of the chromatographic sheet, and then, cooling and curing to construct the micro-fluidic channel; and cutting a corresponding region to obtain the micro-fluidic chip. According to the invention, the chromatographic separation characteristic of the surface of the chromatographic sheet is sufficiently used. A fluid passage of the micro-fluidic chip prepared by taking the chromatographicsheet as a substrate has a thin-layer chromatographic separation function and is capable of increasing the analysis efficiency and improving the accuracy. The production method and equipment are simple, free of special clean environments and capable of realizing rapid and mass replication.

Description

technical field [0001] The invention belongs to the technical field of microfluidic chips, and in particular relates to a microfluidic chip based on a thin-layer chromatographic plate and a manufacturing method thereof. Background technique [0002] Microfluidic chip (Microfluidic chip) is a science and technology characterized by the manipulation of fluids in the micron-scale space. Called a chip laboratory (Lab-on-a-Chip). At present, the mainstream form of microfluidic chips is mostly composed of microchannels to form a network, with controllable fluid running through the entire system, to realize various basic functions of conventional chemical or biological laboratories, such as: sample injection, mixing, reaction, separation and detection etc. The basic feature and biggest advantage of microfluidic chips is the flexible combination and integration of various unit technologies on a small and controllable platform. [0003] The core of microfluidic chip technology lie...

Claims

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Application Information

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IPC IPC(8): B01L3/00
CPCB01L3/502707B01L3/502753B01L2300/0809B01L2300/0887B01L2200/0631B01L2200/10B01L2200/12
Inventor 丁宗庆章平平王振忠
Owner 汉江师范学院