Method and apparatus for detecting pathogen distribution and drug resistance of pathogen species

Abstract

The invention relates to a method for detecting pathogen distribution and drug resistance of pathogen species, including the steps: obtaining a pathogen sample by centrifugation or filtration from a body fluid known to contain or possibly contain pathogen cells; taking out a certain amount of pathogen sample and antibiotic respectively, dripping at least one contain at the same time or step by step, wherein the bottom of the contain is provided with a planar quadrupole gold microelectrode array, and a voltage signal applied on the microelectrode can generate a non-uniform electric field, so that pathogen cells form a dielectrophoretic directional movement, and aggregate into several to several hundred pathogen cell aggregates toward the center of the bottom of the container. By performingthree-dimensional Raman spectroscopic imaging on the pathogen cell aggregates by laser scanning, it is possible to distinguish different pathogen cell distributions and drug resistance situations in the pathogen cell aggregates. The present invention also provides an apparatus for realizing the above-mentioned detection of pathogen distribution and drug resistance of pathogen species. Through thereal-time detection and analysis of these Raman spectra, the pathogen species distribution and drug resistance screening are carried out.

Description

Technical field [0001] The invention relates to the field of physics, in particular to an optical detection technology, in particular to a method and device for detecting the distribution and drug resistance of pathogens. Background technique [0002] At present, in clinical infection medicine diagnosis and treatment, because it is impossible to quickly determine the type and resistance of pathogens, doctors often use broad-spectrum antibiotics and combined antibiotics based on their experience, often with uncertain efficacy, causing antibiotic abuse problems. In recent years, the problem of antibiotic abuse has become more and more serious, and the resistant and resistant bacteria produced by the abuse of antibiotics have ravaged the world. The latest statistics point out that drug-resistant bacteria cause 700,000 deaths worldwide each year, mostly in developing countries. According to the figures, by 2050, the number of deaths worldwide could reach 10 million. [0003] Since the...

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to provide a method for detecting the distribution of pathogen species and drug resistance, and the method for detecting the distribution and drug resistance of pathogen species will solve the method for measuring the type and drug resistance of pathogens in the prior art Complicated, poorly performing technical issues

Images