Dendrobium officinale protocorm culture medium and application thereof
A technology of Dendrobium officinale and culture medium, which is applied in the field of proliferation and differentiation culture medium of Dendrobium officinale protocorm, can solve the problems of poor sunlight adaptability, uneven distribution, low reproduction coefficient, etc. The effect of uniform and increased multiplication coefficient
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Embodiment 1
[0109] A protocorm culture medium of Dendrobium officinale, including induction medium, proliferation and differentiation medium;
[0110] The induction medium is composed of the following components: MS basic medium, 6-benzylaminoadenine 1.6 mg / L, naphthaleneacetic acid 0.8mg / L, PQQ 1.5umol / L, plant extract mixture 28mg / L, sucrose 120 mg / L, medium functional solution 8mg / L;
[0111] The proliferation and differentiation medium consists of the following components: 1 / 2 MS basic medium, 6-benzylaminoadenine 2.5mg / L, naphthaleneacetic acid 0.6 mg / L, PQQ 0.5umol / L, plant extract mixture 16 mg / L L, sucrose 40 mg / L, vitamin B2 0.2 mg / L; medium functional solution 5 mg / L;
[0112] Among them, the plant extract is composed of mulberry leaf extract, Scutellaria baicalensis extract and tannin; the mulberry leaf powder processed from the 1st to 3rd new leaves on the mulberry branches of the mulberry leaf extract is used as raw material, dried in the shade, pulverized, Alcohol, 90% et...
Embodiment 2
[0118] A kind of dendrobium officinale protocorm culture medium, is characterized in that: comprise induction medium, proliferation and differentiation medium;
[0119] The induction medium is composed of the following components: MS basic medium, 6-benzylaminoadenine 2.5mg / L, naphthaleneacetic acid 0.6 mg / L, PQQ 0.5 umol / L, plant extract mixture 16mg / L, sucrose 80mg / L L, medium functional solution 5 mg / L;
[0120] The proliferation and differentiation medium consists of the following components: 1 / 2 MS basic medium, 6-benzylaminoadenine 1.6 mg / L, naphthaleneacetic acid 0.8mg / L, PQQ 1.5umol / L, plant extract mixture 28mg / L , sucrose 60 mg / L, vitamin B2 0.3mg / L; medium functional solution 8mg / L;
[0121] Among them, the plant extract is composed of mulberry leaf extract, Scutellaria baicalensis extract and tannin; the mulberry leaf powder processed from the 1st to 3rd new leaves on the mulberry branches of the mulberry leaf extract is used as raw material, dried in the shade, p...
Embodiment 3
[0127] A protocorm culture medium of Dendrobium officinale, including induction medium, proliferation and differentiation medium;
[0128] The induction medium is composed of the following components: MS basic medium, naphthaleneacetic acid 0.7mg / L, PQQ 0.8umol / L, plant extract mixture 23mg / L, sucrose 110 mg / L, medium functional liquid 6mg / L ;
[0129] The proliferation and differentiation medium consists of the following components: 1 / 2 MS basic medium, 6-benzylaminoadenine 1.8mg / L, naphthaleneacetic acid 0.7mg / L, PQQ 0.7umol / L, plant extract mixture 22mg / L , sucrose 50 mg / L, vitamin B2 0.25 mg / L; medium functional solution 6 mg / L;
[0130] Among them, the plant extract is composed of mulberry leaf extract, Scutellaria baicalensis extract and tannin; the mulberry leaf powder processed from the 1st to 3rd new leaves on the mulberry branches of the mulberry leaf extract is used as raw material, dried in the shade, pulverized, Alcohol, 90% ethanol and water heating extraction,...
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