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Dendrobium officinale rooting culture medium and application thereof

A rooting medium and technology of Dendrobium officinale, which is applied in the field of proliferation and differentiation medium of Dendrobium officinale protocorm, can solve the problems affecting the thickness of Dendrobium officinale stem sections, rooting rate and seedling rate, low growth rate of Dendrobium officinale, transplanting Problems such as low survival rate, to achieve the effect of easy to pull out, not easy to damage the root, and improve the rooting rate

Inactive Publication Date: 2019-01-15
NANJING LONGYUAN ECOLOGICAL AGRI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional asexual reproduction method has a low reproduction coefficient. The cultivation of protocorms goes through three stages of induction, differentiation and multiplication. The quality of protocorm cultivation directly affects the thickness of stem segments, rooting rate and seedling rate of Dendrobium officinale
[0003] In the prior art, Dendrobium candidum still has many problems such as low growth rate and low transplant survival rate.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] A rooting medium for Dendrobium officinale, the rooting medium is composed of the following components: improved MS basic medium + indole butyric acid IBA0.5mg / L+PQQ1.5umol / L+ diethyl aminohexanoate 0.1mg / L+ banana Gravy 15g / L+citric acid 12g / L+plant extract mixture 18mg / L+vitamin B2 0.2 mg / L+medium functional solution 8mg / L.

[0042] The improved MS basal medium consists of the following components:

[0043] Ammonium nitrate NH 4 NO 3 300mg / L

[0044] Calcium nitrate Ca(NO 3 ) 2 4H 2 O 328 mg / L

[0045] Calcium Chloride CaCl 2 2H 2 O 98mg / L

[0046] Magnesium Sulfate MgSO 4 ·7H 2 O 185 mg / L

[0047] Potassium dihydrogen phosphate KH 2 PO 4 85mg / L

[0048] Potassium sulfate K 2 SO 4 495mg / L

[0049] Disodium EDTA 2 EDTA 37.3mg / L

[0050] Ferrous Sulfate FeSO 4 ·7H 2 O 27.8 mg / L

[0051] Manganese sulfate MnSO 4 ·H 2 O 22.3 mg / L

[0052] Zinc sulfate ZnSO 4 ·7H 2 O 8.6 mg / L

[0053] boric acid H 3 BO 3 8.2 mg / L

[0054] Sodium molybdat...

Embodiment 2

[0066] A rooting medium for Dendrobium officinale, the rooting medium is composed of the following components: improved MS basic medium + indole butyric acid IBA2.0mg / L+PQQ 0.5 umol / L + diethyl aminohexanoate 0.02mg / L+ banana Gravy 5g / L+citric acid 5g / L+plant extract mixture 12mg / L+vitamin B2 0.3 mg / L+medium functional solution 5mg / L.

[0067] The improved MS basal medium consists of the following components:

[0068] Ammonium nitrate NH 4 NO 3 300mg / L

[0069] Calcium nitrate Ca(NO 3 ) 2 4H 2 O 328 mg / L

[0070] Calcium Chloride CaCl 2 2H 2 O 98mg / L

[0071] Magnesium Sulfate MgSO 4 ·7H 2 O 185 mg / L

[0072] Potassium dihydrogen phosphate KH 2 PO 4 85mg / L

[0073] Potassium sulfate K 2 SO 4 495mg / L

[0074] Disodium EDTA 2 EDTA 37.3mg / L

[0075] Ferrous Sulfate FeSO 4 ·7H 2 O 27.8 mg / L

[0076] Manganese sulfate MnSO 4 ·H 2 O 22.3 mg / L

[0077] Zinc sulfate ZnSO 4 ·7H 2 O 8.6 mg / L

[0078] boric acid H 3 BO 3 8.2 mg / L

[0079] Sod...

Embodiment 3

[0091] A rooting medium for Dendrobium officinale, the rooting medium is composed of the following components: improved MS basic medium + indole butyric acid IBA0.8mg / L+PQQ 0.9umol / L + diethyl aminoethyl hexanoate 0.07mg / L+ banana Gravy 12g / L+citric acid 8g / L+plant extract mixture 15mg / L+vitamin B2 0.25 mg / L+medium functional solution 7mg / L.

[0092] The improved MS basal medium consists of the following components:

[0093] Ammonium nitrate NH 4 NO 3 300mg / L

[0094] Calcium nitrate Ca(NO 3 ) 2 4H 2 O 328 mg / L

[0095] Calcium Chloride CaCl 2 2H 2 O 98mg / L

[0096] Magnesium Sulfate MgSO 4 ·7H 2 O 185 mg / L

[0097] Potassium dihydrogen phosphate KH 2 PO 4 85mg / L

[0098] Potassium sulfate K 2 SO 4 495mg / L

[0099]Disodium EDTA 2 EDTA 37.3mg / L

[0100] Ferrous Sulfate FeSO 4 ·7H 2 O 27.8 mg / L

[0101] Manganese sulfate MnSO 4 ·H 2 O 22.3 mg / L

[0102] Zinc sulfate ZnSO 4 ·7H 2 O 8.6 mg / L

[0103] boric acid H 3 BO 3 8.2 mg / L

[0104] Sodiu...

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Abstract

The invention discloses a dendrobium officinale rooting culture medium. The rooting culture medium consists of the following components: an improved MS basic culture medium, 0.5-2.0 mg / L of indolebutyric acid IBA, 0.5-1.5 [mu]mol / L of PQQ, 0.02-0.1 mg / L of 2-diethylaminoethyl hexanoate, 5-15 g / L of a banana pulp juice, 5-12 g / L of citric acid, 0.2-0.3 mg / L of vitamin B2, 12-18 mg / L of a plant extract mixture, and 5-8 mg / L of a culture medium functional solution. The plant extract mixture in the culture medium provided by the invention includes a mulberry leaf extract, a water chestnut leaf juice and tannin and a specific proportion, and can make the root stress resistance strong. After formed seedlings are transplanted, the transplanting survival rate reaches nearly 100%, and no root rotting or root dysplasia happens.

Description

technical field [0001] The invention belongs to the field of dendrobium cultivation, and in particular relates to a proliferation and differentiation medium for dendrobium candidum protocorms and an application thereof. Background technique [0002] Dendrobium officinale (Deudrobium officiuale) is a perennial epiphytic herb of the genus Dendrobium orchidaceae. Qi, long muscles, intelligence and shock, light body and prolong life"; folks call it "life-saving celestial grass". Most of them are distributed on the semi-shady rocks in the mountains with an altitude of nearly 1,000 meters. In addition to long-term excavation, the resources of wild Dendrobium officinale are gradually depleted. Because the seeds have no endosperm and need to be combined with symbiotic bacteria to germinate normally, the cultivation of seedlings is difficult. The traditional asexual reproduction method has a low reproduction coefficient. The cultivation of protocorms goes through three stages of in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 左晓燕
Owner NANJING LONGYUAN ECOLOGICAL AGRI CO LTD