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Saccharomyces cerevisiae strain, method and application for producing high nucleic acid yeast

A technology for Saccharomyces cerevisiae and nucleic acid yeast, which is applied in microorganism-based methods, biochemical equipment and methods, and medical preparations containing active ingredients, etc., can solve the problem of low nucleic acid content, increase production costs, and cannot meet large-scale industrial production. and other problems, to achieve the effect of increasing nucleic acid content and reducing production costs

Active Publication Date: 2021-09-28
ANGELYEAST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The problem of the prior art that the present invention solves is: utilize existing yeast strain to carry out large-scale industrial production, the nucleic acid content of the yeast that it obtains is not high, can't meet the demand of large-scale industrial production, the yeast that these industrial production obtains , because the nucleic acid content is not high, when it is used in medicine, health food or infant food, it increases the corresponding production cost

Method used

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  • Saccharomyces cerevisiae strain, method and application for producing high nucleic acid yeast
  • Saccharomyces cerevisiae strain, method and application for producing high nucleic acid yeast
  • Saccharomyces cerevisiae strain, method and application for producing high nucleic acid yeast

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Embodiment 1

[0061] (1) Fermentation production of high nucleic acid yeast

[0062] The invention provides a method for producing yeast by utilizing Saccharomyces cerevisiae yeast strain d8.8, comprising the following steps:

[0063] The Saccharomyces cerevisiae d8.8 (Saccharomyces cerevisiae d8.8) strain was used and preserved in the China Center for Type Culture Collection (CCTCC) with the preservation number CCTCC M2017148. Observed by an optical microscope, the cell diameter of the Saccharomyces cerevisiae strain is about 4-6 μm, presents an ellipsoid, and reproduces asexually by budding; after being cultured on a solid medium plate at 28°C for 24 hours, it becomes a milky white, opaque, round colony.

[0064] After being activated by using the described bacterial strains, culture in 250mL shake flasks, 10L small tanks, 15mL 3 Fermentation tank culture, 60m 3 First-class seed fermentation tank cultivation, 300m 3 Secondary seed fermenter cultivation and 300m 3 Commercial fermentati...

Embodiment 2

[0126] (1) Preparation of high nucleic acid yeast

[0127] The following method is adopted for industrial production to obtain high nucleic acid yeast.

[0128] (1) The culture steps of 250mL shake flask are as follows: the slant bacteria were inoculated into the 250mL shake flask, and cultured at 35°C for 20h.

[0129] Among them, the formula of 250mL medium is as follows: 20g of sucrose, 2g of potassium dihydrogen phosphate, 2g of magnesium sulfate, 1g of yeast extract powder and 100mL of sterile water, and adjust the pH value to 6.

[0130] (2) Cultivation in a 10L small tank is as follows: transfer the shake flask culture solution obtained in step (1) into a 10L small tank, and culture at a temperature of 35° C. with stirring at 20 r / min for 20 h to obtain a seed culture solution.

[0131] Among them, the formula of the 10-liter small tank medium is as follows: 1.5Kg of sucrose, 300g of yeast extract powder, 5g of potassium dihydrogen phosphate, 5g of magnesium sulfate an...

Embodiment 3

[0147] (1) Preparation of high nucleic acid yeast

[0148] The following method is adopted for industrial production to obtain high nucleic acid yeast.

[0149] (1) The culture steps of 250mL shake flask are as follows: the slant bacteria were inoculated into the 250mL shake flask, and cultured at 28°C for 30h.

[0150] Among them, the formula of 250mL medium is as follows: 5g of sucrose, 1g of potassium dihydrogen phosphate, 1g of magnesium sulfate, 3g of yeast extract powder and 100mL of sterile water, and adjust the pH value to 5.5.

[0151] (2) Cultivate in a 10L small tank as follows: transfer the shake flask culture solution obtained in step (1) into a 10L small tank, and culture at 28° C. with stirring at 15 r / min for 30 h to obtain a seed culture solution.

[0152] Among them, the formula of the 10-liter small tank medium is as follows: 375Kg of sucrose, 200g of yeast extract powder, 10g of potassium dihydrogen phosphate, 10g of magnesium sulfate and 7.5L of sterile w...

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Abstract

The invention relates to a strain of Saccharomyces cerevisiae, a method for producing high nucleic acid yeast and its application. The invention provides a Saccharomyces cerevisiae d8.8 (Saccharomyces cerevisiae d8.8) strain, which is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: M 2017148. At the same time, it also provides the microbial inoculant obtained by fermenting the strain and the method for producing yeast. The method comprises carrying out shake flask culture, seed culture, primary fermentation, primary fermentation, secondary fermentation and commercial fermentation of bacterial strain d8.8 respectively, so that the final yeast concentration is 180-240g / L. The invention utilizes yeast strains for industrialized fermentation production, and the content of nucleic acid in the yeast cells reaches more than 20% of the dry weight of the cells, thereby increasing the content of nucleic acids in the yeast cells and reducing the cost of industrial production.

Description

technical field [0001] The invention relates to the technical field of microbial application, in particular to a strain of Saccharomyces cerevisiae and a method for producing high nucleic acid yeast and its application. Background technique [0002] Yeast nucleic acid is ribonucleic acid extracted from natural yeast. It is currently mainly used in medicine, health food and infant food. At present, national laws and regulations stipulate that ribonucleic acid can only be extracted from Saccharomyces cerevisiae or Kluyveromyces, but this The nucleic acid content in both yeast strains was very low. The application publication number is CN1019286A which mentions that a high nucleic acid Saccharomyces cerevisiae strain has a nucleic acid content of about 12-14%. [0003] The application publication number is CN102559522A, which proposes a high-nucleic acid baker's yeast and a preparation method thereof. The nucleic acid content of the strain is 20%. [0004] The application pub...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/18A61K36/064C12R1/865
CPCA61K36/064A23L33/14C12N1/18C12N1/185C12R2001/865
Inventor 刘云华俞学锋李知洪姚鹃郑国斌
Owner ANGELYEAST CO LTD
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