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Group I avian adenovirus type 4 strain and application thereof

A poultry adenovirus and virus technology, applied in the direction of viruses, antiviral agents, virus antigen components, etc., can solve the problems of time-consuming and labor-intensive, chicken stress, affecting production performance, etc., and achieve good and strong immunogenicity , stable effect

Active Publication Date: 2019-01-15
乾元浩生物股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Generally, separate vaccines for the above three diseases must be vaccinated at the same time to prevent the outbreak of these diseases in chicken flocks, which is not only time-consuming and laborious, but also causes stress to the chickens and affects production performance

Method used

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  • Group I avian adenovirus type 4 strain and application thereof
  • Group I avian adenovirus type 4 strain and application thereof
  • Group I avian adenovirus type 4 strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Isolation, purification and identification of avian adenovirus ZMXZAV-4 strain

[0037] 1. Epidemiological situation

[0038] Since 2014, an epidemic disease with rapid spread, high mortality and acute onset has appeared in some areas of my country. Chickens have no obvious symptoms before the onset, and acute death occurs soon after the onset. In the early stage, the disease was mainly caused by small-scale farms, and soon large-scale farms also began to suffer from the disease. Because there were no better control measures, the farmers suffered serious economic losses. The disease mainly infects broiler chickens aged 3 to 6 weeks, and the mortality rate is 20% to 80%. Both layer hens and breeder hens are infected. After analysis, it was suspected hydropericardium syndrome, and the pathogen may be avian adenovirus. In 2014, the applicant isolated a strain of virus from a dead chicken with typical characteristics of hydropericardium in a chicken farm in Hena...

Embodiment 2

[0073] Embodiment 2 chicken Newcastle disease, infectious bursal disease, poultry adenovirus triple inactivated vaccine source and standard

[0074] 1. Source of poisonous species

[0075] Chicken Newcastle disease virus kind is chicken Newcastle disease virus La Sota strain, and the virus strain used for effect inspection is chicken Newcastle disease virus virulent Beijing strain (CVCC AV1611 strain), all is identified, kept and supplied by China Veterinary Drug Supervision Institute; Infectious bursal disease virus kind is LY23 strain was identified and kept by China Animal Husbandry Co., Ltd. The strain used for efficacy testing was Infectious Bursal Disease Virus BC6 / 85 strain (CVCC AV7), which was identified, kept and supplied by China Veterinary Drug Control Institute; seedling production and The strain of avian adenovirus used for efficacy testing is ZMXZAV-4, which was identified and kept by China Animal Husbandry Co., Ltd.

[0076] 2. Virus seed standard

[0077] 2....

Embodiment 3

[0149] The preparation of embodiment 3 chicken Newcastle disease, infectious bursal disease, poultry adenovirus triple inactivated vaccine production virus seed

[0150] 1. Preparation of Newcastle Disease Virus La Sota Strain Virus Seed

[0151] Properly dilute the virus seed with sterilized physiological saline or PBS (such as 10 -4 or 10 -5 ), inoculate 10-day-old SPF chicken embryos in the allantoic cavity, 0.1ml per embryo. Choose chicken embryos that died 72 to 120 hours after inoculation and had obvious lesion scars, and harvest the chicken embryo fluid (allantoic fluid and amniotic fluid) respectively, and put them in sterilized containers. Mix the chicken embryo liquid that has been tested for sterility and whose agglutination value for 1% chicken erythrocytes is not less than 1:512 (micro method), and quantitatively distribute it in sterile bottles, and indicate the date of harvest, the generation of the virus species and the filling volume. Store frozen.

[0152...

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Abstract

The invention relates to the technical field of veterinary biological products, in particular to a group I avian adenovirus type 4 strain and application thereof. The group I avian adenovirus type 4 strain ZMXZAV-4 strain, with an accession number of CGMCC No. 14296, has the characteristics of strong cellular adaptability, high toxin production and strong immunogenicity. Newcastle disease virus (NDV), infectious bursal disease virus (IBDV) and avian adenovirus (AAV) are triple inactivated vaccines based on the virus strain, wherein the NDV is La Sota strain, the infectious bursal disease virus(IBDV) strain is LY23 with an accession number of CGMCC No. 11594; the Avian adenovirus is group I avian adenovirus type 4 ZMXZAV-4 strain, with an accession number of CGMCC No. 14296. The vaccine issafe and effective, and can be used for preventing Newcastle disease, infectious bursal disease and avian adenoviral disease, achieving the effect of preventing three diseases by one injection, and reducing immune cost and immune stress reaction.

Description

technical field [0001] The invention relates to the technical field of veterinary biological products, in particular to a group I type 4 avian adenovirus strain and its application in a triple inactivated vaccine of Newcastle disease virus, infectious bursal disease virus and avian adenovirus. Background technique [0002] With the rise of intensive poultry farming, the epidemic characteristics of poultry diseases are becoming more and more complicated. Often many old diseases are not effectively controlled, and new diseases continue to occur, which has always restricted the healthy and stable development of the poultry industry. [0003] Newcastle disease is an acute, highly contagious infectious disease with high morbidity and mortality. Once an outbreak occurs, it will bring serious losses to the poultry industry. OIE lists it as a reportable disease. [0004] Avian IBD is a highly contagious disease of chickens and turkeys caused by IBD virus, mainly affecting the lympho...

Claims

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Application Information

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IPC IPC(8): C12N7/00A61K39/17A61K39/12A61K39/235A61K39/295A61P31/14A61P31/20A61P9/00A61P1/16C12R1/93
CPCA61K39/12C12N7/00A61K2039/552A61K2039/5252A61K2039/70C12N2710/10234C12N2710/10221A61K2300/00
Inventor 张贺楠张钊伟于雷邵葳周建民史张艳程海波
Owner 乾元浩生物股份有限公司
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