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Ultraviolet LED automatic switching device for fluorescence detection

A technology of automatic switching and fluorescence detection, applied in measurement devices, fluorescence/phosphorescence, material analysis by optical means, etc., can solve problems such as low efficiency, improve detection efficiency, shorten the time of optical path debugging, ensure accuracy and repetitive effect

Pending Publication Date: 2019-01-15
DALIAN ELITE ANALYTICAL INSTR
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  • Abstract
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Problems solved by technology

However, the existing ultraviolet LED excitation method is to use a certain wavelength of ultraviolet LED to perform fluorescence excitation detection on a sample to be tested, and the efficiency is low.

Method used

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  • Ultraviolet LED automatic switching device for fluorescence detection
  • Ultraviolet LED automatic switching device for fluorescence detection

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Embodiment Construction

[0008] The ultraviolet LED automatic switching device for fluorescent detection of the present invention has a platform 1, on which a stepping motor 2 is fixed, and the output shaft 3 of the stepping motor 2 is slidably connected with the support 4 through bearings, etc., and on the output shaft 3 A turntable 5 is fixed, and a plurality of ultraviolet LEDs 6 of different wavelengths are arranged at intervals on the circumference of the turntable 5, and the number of ultraviolet LEDs 6 can be determined according to needs. An optical filter 7 is arranged on the ultraviolet LED6, a positioning part 8 is also provided on the rotating disk 5, and a sensing element 9 corresponding to the positioning part 8 is provided on the platform 1, and the output signal of the sensing element 9 is connected with the motor control circuit ( Motor drive and MSP430 microcontroller, etc.). A slip ring 10 is slidably connected to the output shaft 3, which is used to connect lines such as a power su...

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PUM

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Abstract

The invention discloses an ultraviolet LED automatic switching device for fluorescence detection. The ultraviolet LED automatic switching device comprises a platform, a stepping motor is fixed on theplatform, an output shaft of the stepping motor is in sliding connection with a bracket, a rotating plate is fixed on the output shaft, multiple ultraviolet LEDs with different wavelengths are arranged on the circumference of the rotating plate at intervals, and optical filers are arranged on the ultraviolet LEDs; a positioning part is further arranged on the rotating plate, a sensing element corresponding to the positioning part is arranged on the platform, and a sliding ring is connected on the output shaft in a sliding way. The ultraviolet LEDs with different wavelengths (200nm-500nm) can be automatically switched to irradiate different to-be-detected samples so as to perform laser fluorescence detection. The precise positioning can be realized, an optical path axis system can be automatically calibrated so as to guarantee the accuracy and repeatability of the output wavelengths of the ultraviolet LEDS with different wavelengths, the optical path debugging time is greatly shortened,and the detection efficiency is effectively improved.

Description

technical field [0001] The invention relates to a fluorescence excitation device, in particular to an ultraviolet LED automatic switching device for fluorescence detection which can improve detection efficiency. Background technique [0002] At present, the detection methods for mycotoxins and other substances in food mainly include fluorescence detection, enzyme-linked immunoassay (ELISA), thin-layer chromatography (TLC), gas chromatography (GC), liquid chromatography (LC) and liquid chromatography. Chromatography-mass spectrometry (LCMS), etc., among which fluorescence detection characterized by low detection limit has unique advantages. The fluorescence detection method is to put the extracted serum of the sample to be tested into the sample cell, and irradiate the sample cell with an excitation light source to excite the sample; the fluorescence excited by the sample to be tested in the sample cell is focused and filtered and then incident on the PMT spectrum Detection ...

Claims

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Application Information

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IPC IPC(8): G01N21/01G01N21/64
CPCG01N21/01G01N21/64G01N2201/061
Inventor 王文晶韩雪董智勇于德秀唐涛李彤
Owner DALIAN ELITE ANALYTICAL INSTR
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