76 results about "Laser fluorescence" patented technology

The invention relates to a real-time three-dimensional laser fluorescence microscopic imaging device. The real-time three-dimensional laser fluorescence microscopic imaging device is compatible with a traditional bright field illumination microscopic imaging working mode. The real-time three-dimensional laser fluorescence microscopic imaging device is divided into a laser excitation module, a bright field imaging illumination module, a digital detection module, an eyepiece observation module and a control module according to functions. A spatial light modulator is adopted to load a pyramid phase, so that a Gaussian-Bessel illumination light field can be generated at the backfield of a focusing objective lens. In a detection optical path, a twisted Dammann grating is additionally adopted so as to realize simultaneous imaging of a multi-surface object, fluorescence collected from the objective lens simultaneously images a plurality of axial planes to an electron-enhanced CCD detection surface through a multi-surface imaging technology, and therefore, real-time three-dimensional fluorescence imaging can be realized. The real-time three-dimensional fluorescence imaging technique has an important practical value in biological living tissue and living cell microscopic imaging.

An apparatus images a surface. An imager stage linearly translates the surface in a first direction. A light path has a first end defining an input aperture perpendicular to the first direction and parallel to the surface, and a second end defining an output aperture. A plurality of radiation beams linearly scan and interact in time-multiplexed alternating turns with the surface below the input aperture to produce a time-multiplexed light signal that is collected by the input aperture and transmitted by the light path to the output aperture. A photodetector arrangement detects the light signal at the output aperture. A processor processes the detected time-multiplexed light.

The invention discloses an airborne laser-fluorescence sea oil pollution probing device, which is characterized by comprising a laser, a telescope and a spectrometer. An area of a divergent light of each divergent angle generated by irradiating the surface of a probed object by the laser and a visual area of the telescope can maintain superposition, when the laser emits the laser light to irradiate on the surface of the probed object, the telescope receives fluorescence emitted by the probed object due to stimulation, and the amplified fluorescence is transmitted into the spectrometer by the light receiving terminal of the spectrometer through an observing terminal. The laser is used for emitting an ultraviolet light with a certain wavelength to irradiate on the surface of the probed object, and the fluorescence emitted by the surface of the probed object due to stimulation is amplified through the telescope and received by the spectrometer, and the spectrometer receives the fluorescence and analyzes the spectrum characteristic for analyzing the classification of the probed object; a moving window and a slide-rail support are arranged on an airplane so as to more conveniently and flexibly operate the whole device. The device can be widely applied to sea oil spilling probe in 24 hours, and utilizes the characteristic of the fluorescence with high identification ratio.

A method for detecting trace levels of dissolved organic compounds and leached plastic compounds in drinking water includes the steps of employing deep ultraviolet light-emitting diode induced fluorescence at sensitivity levels of several parts per trillion in real time so that a more compact and inexpensive excitation source, relative to a deep UV laser-induced fluorescence, for fluorescence detection of dissolved organic compounds in water is provided. The deep UV light-emitting diode is operated at an excitation near 265 nm and the laser induced fluorescence is detected at an emission near 450 nm to 500 nm for the dissolved organic compounds and near 310 nm for leached plastic compounds. Optical absorption filters and optical bandpass filters are employed to reduce out-of-band light emitting diode emissions and to eliminate second order optical interference signals for the detection of a fluorescence signal near 450 nm to near 500 nm. The system can measure water contained within a quartz optical cell, within bottled water containers, or in a flowing stream of water.

The laser fluorescent correlating spectrum single molecular analyzer is used in the research of single molecule inside solution in life science, chemistry, physics and other fields. The sample solution is set on cover glass or sample pond; the laser beam, after being beam expanded, filtered in exciting filter, reflected in the dichromatic mirror and focused in the objective of microscope, irradiates the sample solution to excite the molecule under research; the fluorescence the sample solution emits is made to pass through the same objective, the dichromatic mirror, emitting filter to eliminate hybrid light, lens to focus and pinhole coupled to reach the single photon detector; and the signal the single photon detector generates is acquired with the data acquisition card and processed in the computer. The present invention has the features of simple operation, high stability, less light loss, high sensitivity, etc. and may be used in various basic researches.

The invention discloses a laser fluorescence inspection method for recognizing a sequence of a crossed writing and seal stamp. The specific implementation scheme is that blue laser is used as a light source and the laser is irradiated to a crossing position, which needs to be recognized, of a seal stamp and writing stokes. A cut-off long-wave-pass color filter with wave length longer than laser wave length is added at the front of a light sensor, and the light sensor transmits an image acquired through a microscope lens and the color filter to a computer through a data line for observation and photographing. By distinguishing the printing ink which emits fluorescence from the writing stokes which do not emit fluorescence through a method that the blue laser can excite printing ink fluorescence, the goal of recognizing the sequence of forming the seal stamp and the handwriting and typewriting is achieved, and the laser fluorescence inspection method has the characteristics of simplicity, feasibility and small equipment investment.

The invention discloses a sea ice monitoring system and a monitoring method. The system comprises a laser emission system, a laser fluorescence receiving system, a photoelectric conversion system, a signal acquisition system and a control system, wherein the laser emission system is used for emitting laser which is high in power and relatively high in repetition frequency and having a special wavelength to a sea surface; the laser fluorescence receiving system is used for collecting a fluorescence signal from stimulated emission of sea ice, and transmitting the received fluorescence signal to the photoelectric conversion system; the signal acquisition system is used for converting an analog electrical signal into a digital signal and storing the digital signal in the control system; the control system is used for implementing data analysis, processing, comparison and display through a data analysis processing system. The monitoring system and the monitoring method disclosed by the invention can make use of sea ice stimulated fluorescence spectrum databases at different temperatures, and analyze and compare the databases through a computer, so as to efficiently and accurately resolve existence of the sea ice; the monitoring system and method, through determining attenuation degree of fluorescence signal strength in the sea ice, can accurately reflect thickness of the sea ice.

The invention discloses a laser fluorescent induction technology-based real-time in-situ detection device for soil pollution. The detection device comprises a drilling head, a laser emission system, adetection system, a data acquisition processing system and a control system, wherein a probe is arranged on the drilling head, the laser emission system is used for emitting and transferring a pulselaser signal to the probe, a fluorescent signal is generated when a soil pollutant at the probe is simulated by laser and then is transferred to the detection system for detection, and the data acquisition processing system is used for processing detected data and outputting a result in real time, and the control system is connected with the drilling head, the laser emission system and the detection system. The detection device employs an LIF technology to research a pollution site in real time, real-time in-situ detection can be performed on an organic pollutant in soil, and three-dimensionaldirection of the pollutant in the soil is provided in real time.

The invention discloses an ultraviolet and deep ultraviolet optical thin film element double-wavelength laser fluorescence spectrometer, which relates to the technical field of application of an optical thin film element. The spectrometer comprises an ArF laser excitation module, a KrF laser excitation module, a sample chamber, a fluorescence detection module and an experimental synchronous control module, wherein the ArF laser excitation module and the KrF laser excitation module emit and transmit laser; the laser is emitted into the sample surface of the sample chamber to produce fluorescence; the fluorescence generated by the excitation of a sample is emitted out of the sample chamber, is transmitted to the fluorescence detection module in an incidence way to carry out spectrum light split, and is detected by a photoelectric detector to convert a fluorescence signal into an electric signal; and the electric signal is transmitted to the experimental synchronous control module. In the spectrometer, two lasers of ArF laser and KrF laser are used as laser sources, so that the sensitivity of ultraviolet and deep ultraviolet fluorescence spectrum detection is improved, and the requirements of testing of an ultraviolet and deep ultraviolet waveband optical thin film weak fluorescence spectrum can be met.

The invention relates to a waste oil detection system based on LIF technology, which is characterized in that it includes: a laser fluorescence probe module, a pH value probe module, a conductivity detection probe module, a spectrum module, a communication module, a host computer module, and a power supply module; wherein The laser fluorescence probe module described above is connected to the spectrum module, the pH value probe module, the conductivity detection probe module, and the spectrum module are connected to the upper computer module, and the spectrum module, the upper computer module are connected to the power supply module. In the present invention, the aflatoxin spectrum information in the detection sample is firstly collected by the laser fluorescence probe module, the heavy metal content in the detection sample is collected by the conductivity detection probe module, and the pH value in the detection sample is collected by the pH value probe module, which is pretreated and compared with the upper Compare the standard information saved in the computer’s database, and display the comparison result on the screen of the host computer. At the same time, if the sample meets the standard, it will display "this sample meets the standard", otherwise it will display "this sample does not meet the standard".

The invention discloses a blood identification method based on infrared Raman ultraviolet fluorescence super-continuum spectrum. The method comprises six steps of instrument start-up and super-continuum spectrum test sample loading; super-continuum spectrum laser diffuse integrated spectrum testing; Raman fluorescence spectrum test sample loading; infrared laser Raman spectrum and ultraviolet laser fluorescence testing; data analysis and fusion; blood identification and determination and the like. The invention has the following beneficial effects: a Y-type fiber linked two-in-and-two-out fiber multiplex spectrograph is used as hardware, and software adopts piecewise-weighted stacking, thus realizing infrared Raman spectrum, ultraviolet fluorescence spectrum and super-continuum diffuse integrated spectrum data information fusion. A blood identification machine of the invention can be suitable for automatic identification of whole blood, blood plasma and serum.

The invention provides a laser source, a light source mode switching method and a laser projection apparatus. The laser source comprises: a laser for generating a laser beam; a fluorescent wheel for receiving the laser beam and emitting fluorescence in at least one color; a color filter wheel rotating synchronously with the fluorescent wheel and including first and second color filter plates arranged radially along the color filter wheel; a light path guide component arranged on a light path between the fluorescent wheel and the color filter wheel and used for guiding the fluorescence in at least one color to the first or second color filter plate, wherein the first color filter plate is used for receiving the fluorescence in at least one color and outputting light of first spectral width,the second color filter plate is used for receiving the fluorescence in at least one color and outputting light of second spectral width, and the first spectral width is greater than the second spectral width. Applicability of the laser source is improved.

A light projection system for generating an image with three primary colors, each primary color being respectively defined by a first, second and third wavebands. The system includes a first blue laser source emitting a first beam in a fourth waveband, the first blue laser source having a first laser driver, a second blue laser source emitting a second beam having a central wavelength and a fifth waveband, the second blue laser source having a second laser driver, a substrate having a wavelength conversion element for emitting light at a plurality of wavelengths after absorption of a light beam at an excitation wavelength within a fifth waveband of the second blue laser source and a beam combiner for combining the combined first beam and the converted beam, which combination results in a white beam. Dichroic losses can be reduced by using a green phosphor together with red laser assistance.

The electrophoresis system comprises: a 3Dcapillary array micro-chip with multiple separation units, a negative feeding and separation device with micro vacuum pump as the negative pressure source, a high-voltage dc power, and a laser fluorescence detection system. This invention reduces cost greatly, and has well repeatability.

The invention discloses a nonlinear laser fluorescence spectrum real-time identification method, which comprises the following steps: learning a sample spectrum, testing sample spectral classification, extracting ROI in an interested region, preprocessing the spectrum, extracting the fluorescence spectrum characteristics by discrete curvelet transform, forming feature vectors, constructing i classes of support vector machines, and distinguishing the test results by classes. The invention adopts the classification method of the support vector machines, and does not depend on large sample training, the input vector is the low-frequency coefficient part after curvelet decomposition, the number of training samples is small, the number of the support vectors is greatly reduced, so the operation time is shortened and the method has instantaneity. The second-generation curvelet transform adopted by the invention is based on a new support frame, and can provide high-efficient, stable and nearly-optimal sparse representation for the curve function with strangeness. Compared with the traditional method, the method is more effective and has higher identification rate. The invention can identify the spectrum samples with data format and image format, and has better adaptability.

The electrophoresis system comprises: a 3Dcapillary array micro-chip with multiple separation units, a negative feeding and separation device with micro vacuum pump as the negative pressure source, a high-voltage dc power, and a laser fluorescence detection system. This invention reduces cost greatly, and has well repeatability.

The invention discloses a pulse spectrograph based on optical fiber dispersion and a measuring method thereof. A section of optical fiber characterized by large material dispersion and small mode dispersion and waveguide dispersion is taken as a spectrum light-splitting device with a simple optical system and no adjustable mechanism; and the spectral resolution can be adjusted by changing the length of the optical fiber or the sampling time interval of a data acquisition system. By utilizing a simplex photodetector to measure spectrum without a scanning mechanism, the spectrograph has the advantages of simple structure, good measuring consistency, and low requirements for working conditions. The invention is applicable to the measurement of the spectrums of pulsed light signals, such as pulsed laser fluorescence spectrum measurement, pulsed laser Raman spectral measurement and the like.

The invention relates to a method for quantitatively measuring an infinitesimal amount of uranium in steel. The method comprises the following steps: 1, dissolving a steel sample, naturally cooling to 30-40DEG C, and adding water for continuous dissolving to a constant volume; 2, adjusting the pH value of a steel sample solution obtained in step 1 to 1-3, and adding 1-5wt% of an ascorbic acid solution for pretreatment to fully reduce F3<3+> into Fe<2+>; 3, allowing the pretreated steel sample solution to flow through a highly basic anion exchange column for absorbing uranium, leaching the column with deionized water until no blue color appears when a potassium ferricyanide solution is added in a dropwise manner, resolving uranium with a nitric acid solution, collecting the uranium-enriched resolving solution in a volumetric flask, adjusting the pH value to 3-4, and adding a liquid to a constant volume; and 4, determining the concentration of uranium in the resolving solution through a laser fluorescence method, and reckoning the content of uranium in the steel sample. The method has the advantages of simple operation, small sample volume, accurate and reliable measure result, and very wide application prospect.

The invention discloses a nonlinear optical image recognition method based on deep learning and feature enhancement. The method comprises the steps of obtaining a nonlinear optical image sample set generated based on two-photon laser fluorescence and second harmonic; developing a dual-channel adaptive threshold complementary set segmentation algorithm to segment the gland cavity structure in the prostate cancer tissue image; an image classification network model (AlexNet) is improved, and a segmented glandular cavity structure image is used as a newly added signal channel to carry out featureenhancement learning, so that intelligent Gleason classification with higher accuracy and specificity for unmarked sections of prostate cancer tissues is realized.

The invention discloses a device design of a high-sensitivity visible-near infrared dual-channel laser fluorescence microscope and an imaging method thereof. The device comprises a visible light path(A) and a near-infrared light path (B); the imaging method comprises the steps of mechanical calibration, fine adjustment by using Photoshop software and image fusion by using Image-J software. According to the method, visible light and near-infrared fluorescence in the same microscopic region can be observed, and convenient switching is realized. The monochromaticity of the laser is high, and theexcitation energy is gathered along with the increase of the multiplying power; meanwhile, the filtering efficiency of the optical filter combination on background stray light and excitation light ishigh, and the detection sensitivity on fluorescent substances can be improved. According to the invention, visible and near-infrared fluorescence double-channel observation of an ultrathin transparent biological sample is realized, near-infrared fluorescence single-channel observation can be carried out on the surface of an object with any thickness, and an intuitive means is provided for microscopic distribution observation of near-infrared fluorescent substances (especially indocyanine green and derivatives thereof).

The invention provides a fluorescent probe for detecting carbon monoxide. The fluorescent probe has a chemical structural formula as described in the specification. The above fluorescent probe can detect carbon monoxide in a solution, a cell or an organism. The CO fluorescent probe provided by the invention belongs to a small-molecule fluorescent probe. At present, small-molecule fluorescent probes directed at CO recognition are seldom reported; and in particular, fluorescent probes for recognizing CO in endoplasmic reticulums of cell have not been reported yet. According to the fluorescent probe for detecting CO provided by the invention, fluorescence is significantly enhanced when CO is added into the fluorescent probe, and such a result and phenomenon lay a theoretical foundation for biological imaging application of the probe and indicate that the probe has potential application value in the field of laser fluorescence biomarkers. Correspondingly, the detection of CO by the probe of the invention via the fluorescence imaging technology can be used for evaluating and studying the content and physiological functions of CO in cells; and in particular, the probe can also detect COin the endoplasmic reticulums of cells, so the probe has potential application value in the study of the physiological functions of CO in the endoplasmic reticulums of cells.

The invention provides a laser raman and laser fluorescence-based water surface oil film thickness measurement method. The method comprises the following steps that a total attenuation coefficient A is measured in advance; a saturation fluorescence constant C is measured in advance, wherein the value is equal to the ratio of the intensity of an oil saturation fluorescence signal at the oil film covering part at the same distance from the detection system to the surface of the water film to the intensity of a water raman signal after subtracting the fluorescence background in the position without oil film; in a to-be-tested water area, the water raman signal intensity IR after ignoring the fluorescence background in the position without the oil film at the same distance from the water surface to the detection system, and the fluorescent signal intensity If(d) in the position with the wavelength of lambda f at the oil film covering position are measured onsite; and according to a formula, the thickness of the oil film in the water area to be measured is calculated, wherein d is the thickness of the oil film, A is the total attenuation coefficient, and C is the saturation fluorescenceconstant; the thickness of the water surface oil film can be obtained at different distances, the measurement range of the thickness of the oil film is relatively wide, and great significance is achieved for the practical application of the non-contact water surface oil film thickness measurement technology.

The invention discloses a wavelength conversion device and a laser fluorescence conversion type light source with the same. The wavelength conversion device comprises an encapsulating housing and a fluorescence assembly, wherein the fluorescence assembly contains fluorescence materials and is used for wavelength conversion; the encapsulating housing adopts a cuboid shape and is provided with fourside surfaces and a closed end surface; the other end opposite to the closed end surface, of the encapsulating housing is an exit end of an opening; the two opposite side surfaces in the four side surfaces of the encapsulating housing are light incident surfaces, and incident light windows are formed in the two opposite side surfaces; an optical film allowing specific light beams to penetrate is arranged on the surfaces of the incident light windows; the inner surfaces of the other two side surfaces and the inner surface of the closed end surface are specular reflection surfaces; the fluorescence assembly is arranged in a cavity in the encapsulating housing, and is in the shape of a flake; one end of the fluorescence assembly is connected to the closed end surface; the incident light windows face the upper surface and the lower surface of the fluorescence assembly; and one or more lambert reflecting layers are respectively arranged on the upper surface and the lower surface of the fluorescence assembly.

The invention discloses a blood identification method based on ultraviolet fluorescence super-continuum diffuse integrated spectrum. The method comprises six steps of instrument start-up and super-continuum spectrum test sample loading; super-continuum spectrum laser diffuse integrated spectrum testing; fluorescence spectrum test sample loading; ultraviolet laser fluorescence spectrum testing; data analysis and fusion; blood identification and determination and the like. The invention has the following beneficial effects: a Y-type fiber linked two-in-and-two-out fiber multiplex spectrograph is used as hardware, and software adopts weighted stacking of ultraviolet laser fluorescence spectrum and super-continuum diffuse integrated laser spectrum, thus realizing spectrum data information fusion. Due to robustness of the hardware structure and the software analysis, a blood identification machine can be suitable for automatic identification of whole blood, blood plasma and serum.

The invention discloses an ultraviolet LED automatic switching device for fluorescence detection. The ultraviolet LED automatic switching device comprises a platform, a stepping motor is fixed on theplatform, an output shaft of the stepping motor is in sliding connection with a bracket, a rotating plate is fixed on the output shaft, multiple ultraviolet LEDs with different wavelengths are arranged on the circumference of the rotating plate at intervals, and optical filers are arranged on the ultraviolet LEDs; a positioning part is further arranged on the rotating plate, a sensing element corresponding to the positioning part is arranged on the platform, and a sliding ring is connected on the output shaft in a sliding way. The ultraviolet LEDs with different wavelengths (200nm-500nm) can be automatically switched to irradiate different to-be-detected samples so as to perform laser fluorescence detection. The precise positioning can be realized, an optical path axis system can be automatically calibrated so as to guarantee the accuracy and repeatability of the output wavelengths of the ultraviolet LEDS with different wavelengths, the optical path debugging time is greatly shortened,and the detection efficiency is effectively improved.

The invention discloses a light path structure of a laser movie and television photography lighting lamp. The structure comprises a blue semiconductor laser (1), a red semiconductor laser (2), a greensemiconductor laser (3), an LED lamp bead (4), a lens I (5a), a reflector I (6a), a lens i (5b), a reflector i (6b), a yellow-reflection red-green-blue beam combiner (7), a lens II (8a), a lens III (9a), a lens ii (9b), a reflector II (10), an optical filter (11), a large light transmission pipe (12), a reflector III (13), a lens (14),a blue semiconductor laser I (15), a dispersion sheet (16), asmall light transmission pipe (17), a reflector IV (18), a lens IV (19), a lens V (20) and a fluorescent wheel (21). The light path structure with small divergence angle, high power and uniform illumination developed by the invention has the advantages of high brightness, long service life, no pollution, no speckle and the like of a laser fluorescent light source.