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Blood identification method based on infrared Raman ultraviolet fluorescence super-continuum spectrum

A supercontinuum and infrared spectrum technology, applied in Raman scattering, fluorescence/phosphorescence, color/spectral characteristic measurement, etc., can solve the problems affecting the optical properties of blood, and achieve the effect of improving the signal-to-noise ratio

Active Publication Date: 2016-08-10
SHANGHAI INST OF TECHNICAL PHYSICS - CHINESE ACAD OF SCI
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Problems solved by technology

These interference factors will seriously affect the optical properties of blood, making common optical and spectral methods powerless in the identification of blood in sealed test tubes

Method used

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  • Blood identification method based on infrared Raman ultraviolet fluorescence super-continuum spectrum

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Embodiment Construction

[0041] The hardware system structure of the sealed blood identification instrument based on infrared Raman, ultraviolet fluorescence and supercontinuum integrated laser spectroscopy is as follows: figure 1 As shown, the hardware system mainly consists of a power supply module 1, a visible spectrum spectrometer 2, an infrared spectrum spectrometer 3, two-input and two-out optical fibers 5, a main control and data analysis system 7, an integrating sphere 35, a test tube manipulator 10, and a connecting rod 11 , guide rail 12, manipulator motion controller 13, sample outdoor cover 18, bottom plate 22, bracket 29, light source outdoor cover 30, optical fiber collimation connector 31, laser pigtail 32, supercontinuum laser 34, objective lens bracket 39, display Micro objective lens 40, objective lens coupler 41, optical fiber collimator 42, Y-shaped coaxial optical fiber 43, first optical fiber coupler 44, second optical fiber coupler 50, Y-shaped optical fiber 51, infrared narrow l...

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Abstract

The invention discloses a blood identification method based on infrared Raman ultraviolet fluorescence super-continuum spectrum. The method comprises six steps of instrument start-up and super-continuum spectrum test sample loading; super-continuum spectrum laser diffuse integrated spectrum testing; Raman fluorescence spectrum test sample loading; infrared laser Raman spectrum and ultraviolet laser fluorescence testing; data analysis and fusion; blood identification and determination and the like. The invention has the following beneficial effects: a Y-type fiber linked two-in-and-two-out fiber multiplex spectrograph is used as hardware, and software adopts piecewise-weighted stacking, thus realizing infrared Raman spectrum, ultraviolet fluorescence spectrum and super-continuum diffuse integrated spectrum data information fusion. A blood identification machine of the invention can be suitable for automatic identification of whole blood, blood plasma and serum.

Description

technical field [0001] The invention relates to a human and animal blood classification instrument and method, in particular to a sealed blood identification method based on infrared Raman, ultraviolet fluorescence combined with supercontinuous integrated laser spectrum. Background technique [0002] At present, my country adopts a credit system for the import and export of blood and other various biological materials, but the authenticity of various biological materials cannot be directly tested for various reasons. Especially when it comes to special materials such as blood samples, open contact sampling conditions are often not allowed. On the one hand, the blood sample may be contaminated by the testing operation; on the other hand, the pathogenic factors that the blood sample itself may carry will cause occupational exposure to the testing personnel. In view of the above reasons, it is urgent to develop a non-contact rapid detection method for blood samples. [0003] ...

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Application Information

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IPC IPC(8): G01N21/65G01N21/64G01N21/25G01N21/01
CPCG01N21/01G01N21/25G01N21/6402G01N21/65G01N2021/655
Inventor 万雄刘鹏希章婷婷陈学岗张志敏张华明
Owner SHANGHAI INST OF TECHNICAL PHYSICS - CHINESE ACAD OF SCI
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