Insecticidal composition containing afidopyropen and cycloxaprid

[0018] Example 1: Indoor toxicity example

[0019] The test refers to the "Pesticide Indoor Bioassay Test Guidelines", and the indoor toxicity test is used to illustrate whether the combination of dipropenoxyfen and epoxifen has a synergistic effect on aphids and whitefly.

[0020] (1) Determination of joint virulence to aphids

[0021] Insect dip method was used for indoor toxicity determination. Dispensing the technical solutions of diprofen and epoxicon to prepare 5 series of concentrations.

[0022] Choose the nymphs of the green peach aphid (Myzuspersicae) that are cultivated indoors and have the same age. After immersing the target insect in the drug solution for 10 seconds, absorb the excess drug solution with filter paper, and transfer the test insects to normal conditions for rearing. Each treatment is repeated 4 times. 10 insects, treated with water as a control. After 24 hours, the number of dead and alive insects was checked. The test with a mortality rate of less than 20% in the control group was an effective test.

[0023] According to the survey results, the mortality of each treatment is calculated. Calculate the virulence regression equation, lethal concentration, correlation coefficient, etc., and use Sun Yunpei's method to obtain the co-toxicity coefficient (CTC value).

[0024] The measured toxicity index (ATI)=(standard drug LC50/test drug LC50)×100.

[0025] Theoretical toxicity index (TTI) = A drug toxicity index X the percentage of A in the mixture + B drug toxicity index X the percentage of B in the mixture.

[0026] Co-toxicity coefficient (CTC)=[Measured Toxicity Index of Mixture (ATI)/Theoretical Toxicity Index (TTI) of Mixture]×100.

[0027] According to NY/T1154.7-2006 insecticide combined action classification standard: co-toxicity coefficient (CTC) ≥ 120 shows synergistic effect; co-toxicity coefficient (CTC) ≤ 80 shows antagonism; 80 <120 shows an additive effect.

[0028] Table 1 Indoor combined virulence test results of diprofen, epoxifen and their combination on aphids

[0029]

[0030] The laboratory toxicity test results show that when the weight ratio of dipropenoxyfen to epoxifen is 1-80:1-70, it has good virulence to aphids and shows a synergistic effect.

[0031] (2) Determination of combined virulence to Bemisia tabaci and Thrips

[0032] The original drug is formulated into the required test agent, the test method is agar moisturizing leaf soaking method. First, set 5 different concentration gradients for the single agent and each mixed agent (on the basis of the preliminary test results, the mortality of the test insects is set in the range of 5%-90% in a proportional series). The test insects of Bemisia tabaci in the greenhouse were collected from the field, and the cucumber seedlings were raised in the greenhouse for 3 generations, and uniformly uniform 3rd instar nymphs were selected for use. Use a punch to beat the fresh, flat host leaves into leaf discs, and try to avoid selecting large and thick leaf veins when picking. After immersing the leaf disc in the test solution for 10 seconds, take it out to dry, and use tweezers to spread the leaf face down in a flat-bottomed glass tube with agar added. The leaf disc must be closely attached to the agar and the tube wall without leaving any gaps. . Each treatment was repeated 4 times, and the treatment without medicine was set as the control. Place the glass tube with leaf discs on the host plant of the whitefly in the greenhouse, tap the plant or blow with the mouth to make the whitefly enter the tube, 20 per tube, turn the tube mouth down and wait for the whitefly to fly ( Climbing) When entering the leaf disc at the bottom of the tube, plug the mouth with a cotton plug to a position about 15mm away from the bottom of the tube, so that the whiteflies are forcibly placed on the leaf disc at the bottom of the tube. After the test insects are connected for about 10 minutes, check and record the number of live insects in each tube one by one. Place the treated test insects upside down at a temperature of (25±1) ℃, a relative humidity of 60%-80%, and a photoperiod of L: D = 14h: 10h for rearing and observation. Special conditions can be adjusted appropriately for the test environment . After 48 hours of treatment, check and record the number of dead insects in each tube one by one. Those who do not move or do not move normally are considered dead.

[0033] According to the survey results, the mortality of each treatment is calculated. Calculate the virulence regression equation, lethal concentration, correlation coefficient, etc., and use Sun Yunpei's method to obtain the co-toxicity coefficient (CTC value).

[0034] Measured Toxicity Index (ATI) = (Standard Medicine LC 50 /Testing agent LC 5 )X 100.

[0035] Theoretical toxicity index (TTI) = A drug toxicity index X the percentage of A in the mixture + B drug toxicity index X the percentage of B in the mixture.

[0036] Co-toxicity coefficient (CTC)=[Measured Toxicity Index of Mixture (ATI)/Theoretical Toxicity Index (TTI) of Mixture]×100.

[0037] According to NY/T1154.7-2006 insecticide combined action classification standard: co-toxicity coefficient (CTC) ≥ 120 shows synergistic effect; co-toxicity coefficient (CTC) ≤ 80 shows antagonism; 80 <120 shows an additive effect.

[0038] Table 2 Indoor combined virulence test results of diprofen, epoxifen, and their combinations on Bemisia tabaci

[0039]

[0040] The laboratory toxicity test results showed that when the weight ratio of dipropiofen and epoxifen is 1:80-70:1, it has good virulence to aphids and shows a synergistic effect.

[0041] Table 3 Indoor combined toxicity test results of diprofen, epoxifen and their combinations on thrips

[0042]

[0043] Laboratory toxicity test results show that: when the weight ratio of dipropenoxyfen to epoxifen is 1-10:1-10, it has good toxicity to thrips, showing a synergistic effect.

[0044] Example 2: Formulation Example

[0045] (1) 15% dipropenoxyfen·epoxypyrim water dispersible granules

[0046] 3% Diprofen, 12% Epoxypyr, 3% GY-D06, 4% Sodium Lignosulfonate, 3% Open Powder BX (Sodium Dibutyl Zeasulfonate), 4% K-12 (Sodium lauryl sulfate), light calcium is added to 100% by weight. The above-mentioned raw materials are conventionally prepared by the method of preparing water-dispersible granules, namely, mixing and ultra-fine jet pulverization, mixing, and granulating steps to prepare 15% dimethoprim·epoxypyridine water-dispersible granules.

[0047] (2) 35% Dipropenoxyfen·Epoxypyrim WP

[0048] Weigh 10% aceprofen, 25% epoxifen, 5% calcium alkylphenol polyoxyethylene ether sulfonate, 8% alkyl forbidden sulfonate formaldehyde condensate. 3% alkyl tea sulfonic acid Sodium and talc are added to 100% by weight. The above-mentioned raw materials are mixed, ultra-fine jet pulverization, and mixing process steps to prepare a 35% diprofen and epoxifen wettable powder.

[0049] (3) 18% dipropenoxyfen·epoxypyrim suspension

[0050] 6% diprofen, 12% epoxifen, 3% Nongru 1601#, 1% sodium tea sulfonate, 2% magnesium aluminum silicate, 1% magnesium aluminum silicate, 1% ethylene glycol, 1 % Benzoic acid, 1% silicone defoamer, 1% epichlorohydrin, and deionized water are added to 100% by weight. The above-mentioned raw materials are mixed, high-speed shearing and dispersing for 30 minutes, and then sand-milled with a sand mill to obtain an 18% dimethoprim·epoxypyridine suspension.

[0051] (4) 20% dipropenoxyfen·epoxypyrim microemulsion

[0052] 4% diprofen, 16% epoxifen, 5% agricultural milk 2201, 6% Sipan-60#, 4% Tween 60-#, 6% N-methylpyrrolidone, 11% cyclohexanone , 5% n-butanol, 1% epichlorohydrin, dissolve completely and mix uniformly, add deionized water to 100% by weight, and stir to prepare a 20% difenprofen·epoxypyridine microemulsion.

[0053] (5) 14% Bifenthiprole·Epoxypyrim water emulsion

[0054] 4% diprofen, 10% epoxifen, 3% nonylphenol polyoxyethylene (E0=10) ether phosphate, 5% agricultural milk 700#, 5% agricultural milk 1601,2% Tween- 60#, 1% epoxy soybean oil, 2% glycerin, 2% cyclohexanone, 0.4% bentonite, 0.5% sodium benzoate, and deionized water to 100% by weight. The above-mentioned raw materials are mixed, and high-speed shearing emulsification is carried out to prepare a 14% diprofen-epoxypyrim aqueous emulsion.

[0055] (6) 14% Dipropacycline·Epoxypyrim dispersible oil suspension

[0056] 4% diprofen, 10% epoxypyr, 4% sodium lignosulfonate, 3% agricultural milk 700#.8% BY-110, 1% sodium naphthalene sulfonate, 1% bentonite, 4% propylene glycol , Wheat oil is added to 100% by weight. The above-mentioned raw materials are mixed, high-speed shearing and dispersing for 30 minutes, and then sand-milled with a sand mill to obtain a 14% dispersible oil suspension concentrate of dipropenoxyfen·epoxypyridine.

[0057] Example 3: Example of field use effect

[0058] The field test of the efficacy of bipropafen and epoxicon on cucumber whitefly and cabbage aphid.

[0059] In the peak period of target pest damage, spraying is used. Using 20% ​​diprofen-ethyl suspension and 10% epoxypyridin water-dispersible granules as control agents, the experiment set up 9 drug treatments and 1 blank control area, and each treatment was repeated 3 times. Before pesticide application, the insect population base was investigated at five points on the diagonal of each treatment area, with 10 crops at each point. The control effect was investigated 3 days, 7 days, and 14 days after application, the number of live insects was recorded, and the control effect was calculated.

[0060] Table 4 Field efficacy test results of dipropenoxyfen and epoxifen against cabbage aphids

[0061]

[0062] Table 5 Field efficacy test results of diprofen and epoxicon on cucumber Bemisia tabaci

[0063]