Method for efficiently producing ganoderan through semicontinuous liquid culture

A technology of liquid culture and Ganoderma lucidum polysaccharide, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of fermentation and cultivation of rare edible fungi, achieve accelerated reproduction and synthesis of metabolites, and promote growth Synthesis of biomass and metabolites, effects of increasing biomass and polysaccharide yield of Ganoderma lucidum

Active Publication Date: 2019-01-22
黑龙江坤健生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Semi-continuous culture technology has been gradually applied in the field of industrial production, but it is rarely used in the fermentation culture of edible fungi

Method used

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  • Method for efficiently producing ganoderan through semicontinuous liquid culture
  • Method for efficiently producing ganoderan through semicontinuous liquid culture
  • Method for efficiently producing ganoderan through semicontinuous liquid culture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Adopt Ganoderma lucidum variety Zizhi as test material, test according to a kind of semi-continuous liquid culture method of the present invention to efficiently produce Ganoderma lucidum polysaccharide, concrete steps are as follows:

[0040] (1) Activation and multiplication of mother species

[0041] Use an inoculation shovel to dig out the size of 0.5cm preserved on the PDA slope 2 Ganoderma lucidum parent species were inoculated into fresh PDA slant test tubes, and cultured at 28°C for 5 days. The activated Ganoderma lucidum species were inoculated in the center of the PDA plate medium with a puncher with a diameter of 1 cm, and cultured at 28°C for 6 days. ~7d, inoculate after mycelia cover the plate;

[0042] (2) Preparation of strains in shake flasks

[0043] Using a hole puncher with a diameter of 1cm, take 2~3 pieces of mycelia of the same size from the plate and put them into a conical flask filled with liquid medium. The volume of liquid medium is 400mL / 1L...

Embodiment 2

[0056] The present embodiment is in order to verify the influence of the liquid culture medium that the present invention adopts on the mycelium biomass in the ganoderma lucidum seed liquid, and concrete process is as follows:

[0057] (1) Activation and multiplication of mother species (same as Example 1); (2) Preparation of strains in shake flasks and (3) Preparation of seed solution The operation method is the same as in Example 1, and the liquid medium used is replaced by The following types: medium ①glucose 20g / L, peptone 5g / L, yeast extract 4g / L, KH 2 PO 4 2g / L, MgSO 4 1g / L; Medium ② glucose 36g / L, peptone 4g / L, yeast extract 2g / L, KH 2 PO 4 1g / L, MgSO 4 0.5g / L, VB1 0.05g / L; Medium ③Potato juice 15g / L, wheat bran 3g / L, glucose 3g / L, MgSO 4 3.0g / L,KH 2 PO 4 1.5g / L, these media are all derived from the relevant literature of Ganoderma lucidum liquid culture (fermentation culture). The mycelium biomass of the seed liquid was measured, and the results are shown...

Embodiment 3

[0061] This embodiment is to verify the influence of nitric oxide and ethylene synergistic induction treatment of the present invention on the biomass and polysaccharide content of Ganoderma lucidum mycelium, and the specific process is as follows:

[0062] (1) Activation and multiplication of mother species, (2) preparation of strains in shake flasks, (3) preparation of seed liquid, the three steps are the same as in Example 1, (4) one step of synergistic induction treatment of nitric oxide and ethylene The following tests were carried out: ① Nitric oxide induced treatment alone, ② Ethylene alone induced treatment, ③ No treatment, (5) Semi-continuous liquid culture was the same as in Example 1.

[0063] After the fermentation, the mycelium biomass, exopolysaccharide content, and intracellular polysaccharide content of the ganoderma lucidum fermentation liquid were measured, and the results are shown in Table 2 below.

[0064]

[0065] Analysis of the data in Table 2 shows ...

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Abstract

The invention discloses a method for efficiently producing ganoderan through semicontinuous liquid culture. The method comprises the following steps: (1) carrying out activating and expanding propagation on mother strains; (2) preparing strains through shake-flask culture; (3) preparing a seed solution; (4) carrying out synergetic inducing treatment by adopting nitric oxide and ethylene; and (5) carrying out semicontinuous liquid culture. With the semicontinuous liquid culture method, the logarithmic phase of lucid ganoderma mycelia can be effectively prolonged, so that the liquid culture biomass of lucid ganoderma is improved, and the yield of ganoderan is improved. Compared with the conventional batch culture method, the method provided by the invention has the advantages that the process is reasonable, the operation is simple, the non-fermentation time used for repeated tank washing, sterilizing and the like can be saved, thus the production efficiency and the equipment utilizationrate are improved, the production cost is reduced, and the method can be used for producing ganoderan in large scale, and belongs to the liquid culture method for lucid ganoderma with a great application prospect.

Description

technical field [0001] The invention relates to the technical field of production of medicinal fungi, in particular to a method for highly efficient production of ganoderma lucidum polysaccharides by semi-continuous liquid culture. Background technique [0002] Medicinal fungi usually refer to large fungi (mushrooms) used in medicine. Common species include Cordyceps, Cicadae, Ganoderma lucidum, Poria, Polyporus, Phellinus, Fungus, Leiwan, Yunzhi, etc. These medicinal fungi have been used for thousands of years in our country. Although they have different effects, the biggest advantage is that they have no toxic side effects. Modern medical research shows that medicinal fungi not only have the traditional functions of nourishing qi, strengthening the body, dispelling diseases, stimulating the menstrual flow, and prolonging life, but also have the effects of enhancing human immunity and anti-tumor and anti-cancer effects. Therefore, medicinal fungi are one of the most potent...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C12R1/645
CPCC12P19/04
Inventor 郑涛
Owner 黑龙江坤健生物科技有限公司
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