Method for preparing and using capacitive biochip for detecting human immunodeficiency virus
A technology of human immunodeficiency and biochip, which is applied in the field of capacitive biochip preparation to achieve the effect of improving the inspection range, facilitating the use of clinical testing, and improving the precision and inspection range
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Embodiment 1
[0045] A method for preparing a capacitive biochip for detecting human immunodeficiency virus of the present embodiment comprises the following specific steps:
[0046] (1) Preparation and purification of mouse anti-HIV polyclonal antibody
[0047] ①Immunization of BALB / c mice:
[0048] Select 6-week-old female mice weighing more than 25 g, take 1 mL of inactivated HIV antigen (2 mg / mL) and fully homogenate with an equal amount of Freund's adjuvant to form a stable emulsion, which is recorded as emulsified antigen; each mouse is injected intraperitoneally with a total of 0.2 mL emulsified antigen; the second immunization was carried out 7 days later, and the immunization scheme was as before; the third immunization was carried out 21 days later, and the immunization scheme was as before; the fourth immunization was carried out 42 days later, and the immunization scheme was as before; the fifth immunization was carried out 49 days later , the immunization scheme was as before;...
Embodiment 2
[0057] A method for preparing a capacitive biochip for detecting human immunodeficiency virus of the present embodiment comprises the following specific steps:
[0058] (1) Preparation and purification of mouse anti-HIV polyclonal antibody
[0059] ①Immunization of BALB / c mice:
[0060] Select 6-week-old female mice weighing more than 25 g, take 1 mL of inactivated HIV antigen (2 mg / mL) and fully homogenate with an equal amount of Freund's adjuvant to form a stable emulsion, which is recorded as emulsified antigen; each mouse is injected intraperitoneally with a total of 0.2 mL emulsified antigen; the second immunization was carried out 7 days later, and the immunization scheme was as before; the third immunization was carried out 21 days later, and the immunization scheme was as before; the fourth immunization was carried out 42 days later, and the immunization scheme was as before; the fifth immunization was carried out 49 days later , the immunization scheme was as before;...
Embodiment 3
[0069] A method for preparing a capacitive biochip for detecting human immunodeficiency virus of the present embodiment comprises the following specific steps:
[0070] (1) Preparation and purification of mouse anti-HIV polyclonal antibody
[0071] ①Immunization of BALB / c mice:
[0072] Select 6-week-old female mice weighing more than 25 g, take 1 mL of inactivated HIV antigen (2 mg / mL) and fully homogenate with an equal amount of Freund's adjuvant to form a stable emulsion, which is recorded as emulsified antigen; each mouse is injected intraperitoneally with a total of 0.2 mL emulsified antigen; the second immunization was carried out 7 days later, and the immunization scheme was as before; the third immunization was carried out 21 days later, and the immunization scheme was as before; the fourth immunization was carried out 42 days later, and the immunization scheme was as before; the fifth immunization was carried out 49 days later , the immunization scheme was as before;...
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Abstract
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