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Method for improving enzymolysis efficiency of bagasse through metal salt pretreatment strengthened by additives

A technology of enzymatic hydrolysis efficiency and pretreatment, applied in the direction of fermentation, etc., can solve the problems of long enzymatic hydrolysis time, increase the cost of glucose production, etc., and achieve the effect of improving enzymatic hydrolysis efficiency and efficiency

Inactive Publication Date: 2019-01-29
SOUTH CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although different pretreatment methods can improve the subsequent enzymatic hydrolysis efficiency to a certain extent, to obtain higher enzymatic hydrolysis efficiency usually requires higher cellulase dosage and longer enzymatic hydrolysis time, which increases the production cost of glucose, so There is an urgent need to find a glucose production method that can improve enzymatic hydrolysis efficiency and reduce costs

Method used

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  • Method for improving enzymolysis efficiency of bagasse through metal salt pretreatment strengthened by additives
  • Method for improving enzymolysis efficiency of bagasse through metal salt pretreatment strengthened by additives

Examples

Experimental program
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Effect test

Embodiment 1

[0027] Weigh 50 grams of absolute dry bagasse raw material and place it in a closed reactor equipped with a thermocouple, add 500 milliliters of 0.025mol / L MgCl 2 Aqueous solution and 7.5 grams of Tween 80, start stirring, heat up to 160°C quickly and start timing, wait for the reaction time to reach 10 minutes, stop heating, and immediately use condensed water to reduce the reaction to room temperature, and use vacuum filtration method The pretreatment residue is separated off.

[0028] Take 5 grams (on an absolute dry basis) of the pretreatment residue, add 100 FPU of cellulase (Seli II) and 250 mL of acetic acid-sodium acetate buffer solution with pH=4.8 for enzymatic hydrolysis. During the enzymolysis process, the temperature was controlled at 50° C., and the rotation speed was 150 rpm. After 6 hours and 72 hours of enzymatic hydrolysis, 1mL samples were taken out and inactivated for 10min. The glucose concentration in the enzymolysis liquid was measured by high performan...

Embodiment 2

[0030] Weigh 50 grams of absolute dry bagasse raw material and place it in a closed reactor equipped with a thermocouple, add 500 milliliters of 0.025mol / LFeCl 2 Aqueous solution and 7.5 grams of Tween 80, start stirring, heat up to 160°C quickly and start timing, wait for the reaction time to reach 10 minutes, stop heating, and immediately use condensed water to reduce the reaction to room temperature, and use vacuum filtration method The pretreatment residue is separated off.

[0031] Take 5 grams (on an absolute dry basis) of the pretreatment residue, add 100 FPU of cellulase (Seli II) and 250 mL of acetic acid-sodium acetate buffer solution with pH=4.8 for enzymatic hydrolysis. During the enzymolysis process, the temperature was controlled at 50° C., and the rotation speed was 150 rpm. After 6 hours and 72 hours of enzymatic hydrolysis, 1mL samples were taken out and inactivated for 10min. The glucose concentration in the enzymolysis liquid was measured by high performanc...

Embodiment 3

[0033] Weigh 50 grams of absolute dry bagasse raw material and place it in a closed reactor equipped with a thermocouple, add 500 milliliters of 0.025mol / LZnCl 2 Aqueous solution and 7.5 grams of Tween 80, start stirring, heat up to 160°C quickly and start timing, wait for the reaction time to reach 10 minutes, stop heating, and immediately use condensed water to reduce the reaction to room temperature, and use vacuum filtration method The pretreatment residue is separated off.

[0034] Take 5 g (on an absolute dry basis) of the pretreatment residue, add 100 FPU of cellulase (Seli II) and 250 mL of acetic acid-sodium acetate buffer solution with pH=4.8 for enzymatic hydrolysis. During the enzymolysis process, the temperature was controlled at 50° C., and the rotation speed was 150 rpm. After 6 hours and 72 hours of enzymatic hydrolysis, 1mL samples were taken out and inactivated for 10min. The glucose concentration in the enzymolysis solution was measured by high performance ...

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Abstract

The invention provides a method for improving the enzymolysis efficiency of bagasse through metal salt pretreatment strengthened by additives. The method comprises the following steps including metalsalt pretreatment strengthened by additives on the bagasse raw material, enzymolysis and the like. The method comprises the following steps: mixing the bagasse raw material after air drying and grinding and a metal salt solution with the concentration being 0.01-0.05 mol / L, adding the additives according to the ratio of 100-200 mg / g substrate, carrying out reaction for 5-15 min at 150-170 DEG C for pretreatment, so that the pretreated residue rich in cellulose is obtained, and adding cellulase into the residue for enzymolysis, so that the enzymolysis efficiency is improved. Through the metal salt pretreatment strengthened by additives, the enzymolysis efficiency is improved, the production cost is reduced, and the application prospect is good.

Description

technical field [0001] The invention relates to a method for high-efficiency conversion and utilization of biomass, in particular to a method for improving the enzymatic hydrolysis efficiency of bagasse raw materials through additive-enhanced metal salt pretreatment. Background technique [0002] As energy shortages and environmental issues become increasingly prominent, biomass, as a renewable resource that can be converted into liquid fuels, is receiving increasing attention. Among the abundant biomass resources on the earth, the production of liquid fuels from lignocellulosic biomass has attracted the most attention. [0003] The method of producing liquid fuel from lignocellulosic biomass is to hydrolyze hemicellulose and cellulose in the raw material into monosaccharides such as xylose and glucose, and produce ethanol through fermentation, or synthesize liquid fuel such as fuel oil through catalysis. However, because the cellulose in the biomass raw material is closely...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P19/02
CPCC12P19/02C12P19/14
Inventor 张红丹陈威张嘉杰
Owner SOUTH CHINA AGRI UNIV
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