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A kind of compound fermented protein feed and preparation method thereof

A protein feed and compound fermentation technology, applied in biochemical equipment and methods, animal feed, animal feed, etc., can solve the problems of polluted environment, pomace becoming sour and smelly, and low protein content, so as to purify the environment and inhibit breeding , the effect of increasing protein content

Active Publication Date: 2022-03-15
宁波中瑞生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At the same time, my country is the largest country in apple production, which leads to more than 1 million tons of apple pomace being discharged every year. In addition to the pomace of other fruits, the pomace of other fruits is as high as several million tons; Pomace has a high moisture content, low protein content, and rich content of soluble sugar, vitamins, minerals and cellulose. It is a good nutritional substrate for microorganisms. Therefore, if it is not treated in time, the pomace will soon become sour and smelly. polluted environment

Method used

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  • A kind of compound fermented protein feed and preparation method thereof
  • A kind of compound fermented protein feed and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0035] A preparation method for compound fermented protein feed, comprising the steps of:

[0036] (1) Preparation of strains

[0037] The preparation method of Candida utilis strain (ACCC20060) comprises the steps of: inserting Candida utilis into wort medium, initial pH 6.8~7.0, 26°C, 200r / min shaking culture for 24~ 36h, the number of viable bacteria reached 2.0×10 8 More than cfu / g; Candida utilis medium components are as follows: yeast extract 3g / L, malt extract 3g / L, peptone 5g / L, glucose 10g / L, water to 1L;

[0038] The preparation method of Bacillus subtilis liquid (ACCC11025) comprises the following steps: insert Bacillus subtilis into Bacillus subtilis culture medium, initial pH 7.0, 35°C, 200r / min shaking culture for 24-36h, the number of viable bacteria reaches 1.8× 10 8More than cfu / g; Bacillus subtilis medium components are as follows: glucose 25.0g / L, peptone 5.0g / L, beef extract 3.0g / L, yeast extract powder 1.0g / L, MgSO 4 ·7H 2 O 0.5g / L, MnSO 4 ·H 2 O 0....

Embodiment 2

[0045] A preparation method of compound protein fermented feed, comprising the steps of:

[0046] (1) Preparation of strains

[0047] Step is with embodiment 1

[0048] (2) Preparation of compound fermentation broth

[0049] Step is with embodiment 1

[0050] (3) Preparation of compound protein fermented feed

[0051] Fully mix 50 parts of bean dregs (moisture content 30-40%), 25 parts of apple pomace (moisture content 25-30%), 15 parts of corn flour and 10 parts of bran to prepare the mixed material to be fermented; ) with the compound fermentation bacteria liquid prepared in ) to inoculate the above-mentioned mixed material to be fermented according to the inoculum amount of 15% by weight, and add water to adjust the final moisture content of the material to 50%, fully mix and place it still for 1-2 days; then put the material Put it into a packaging bag with a one-way outlet valve device, seal it, and ferment it statically at 28-35°C for 3-5 days to make a compound ferm...

Embodiment 3

[0053] A preparation method of compound protein fermented feed, comprising the following steps:

[0054] (1) Preparation of strains

[0055] Step is with embodiment 1

[0056] (2) Preparation of compound fermentation broth

[0057] Step is with embodiment 1

[0058] (3) Preparation of compound protein fermented feed

[0059] Fully mix 60 parts of bean dregs (moisture content 30-40%), 20 parts of apple pomace (moisture content 25-30%), 10 parts of corn flour and 10 parts of bran to prepare the mixed material to be fermented; ) to inoculate the above-mentioned mixed material to be fermented with the inoculum amount of 10% by weight, and add water to adjust the final moisture content of the material to 45%, mix well and leave it still for 1 to 2 days; then put the material into Put it into a packaging bag with a one-way outlet valve device, seal it, and ferment it statically at 28-35°C for 3-5 days to make a compound fermented protein feed.

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Abstract

The invention discloses a compound fermented protein feed and a preparation method thereof. The preparation method comprises the following steps: (1) the fermentation bottom material is prepared according to 40-70 parts of bean dregs, 20-30 parts of apple pomace, and 5-15 parts of corn flour. , 5~15 parts of wheat bran are mixed and stirred evenly, and stand-by; (2) the Candida utilis bacterium liquid, the Bacillus subtilis liquid and the Aspergillus niger liquid are mixed evenly, and the compound fermentation bacterium liquid is obtained; (3) in step (1) Inoculate the compound fermented bacterial liquid described in step (2) into the fermented bottom material, mix evenly, seal and pack the material, and ferment statically to obtain a compound fermented protein feed. The finished bean dregs compound fermented protein feed product prepared by the present invention does not need to be dried, has low production cost, high content of probiotics, and has the effect of significantly improving animal intestinal health and improving animal immunity; at the same time, it can also greatly reduce the impact of bean dregs and apple pomace. The pollution caused by the environment has greatly alleviated the shortage of protein feed resources.

Description

technical field [0001] The invention relates to the field of biological fermentation feed processing, in particular to a compound fermented protein feed and a preparation method thereof. Background technique [0002] my country is a large animal husbandry and aquaculture country. With the high-speed growth of my country's aquaculture industry for many years, the shortage of feed resources, especially protein resources, is becoming more and more serious. According to the analysis of relevant experts from the Feed Research Institute of the Chinese Academy of Agricultural Sciences, by 2020, the annual supply and demand gap of protein feed in my country will reach 48 million tons. Therefore, it is difficult to meet the needs of the rapid development of aquaculture by utilizing existing conventional vegetable protein raw materials (such as soybean meal, rapeseed meal, etc.) and animal protein raw materials. Therefore, according to my country's national conditions, how to expand ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K10/37A23K10/30A23K10/12C12N1/16C12N1/14C12N1/20C12R1/72C12R1/125C12R1/685
CPCA23K10/12A23K10/30A23K10/37C12N1/14C12N1/16C12N1/20Y02P60/87
Inventor 方银兵
Owner 宁波中瑞生物科技有限公司
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