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Streptomyces diastaticus and applications thereof

A streptomyces amylase, bacterial technology, applied in the field of microorganisms, can solve limited problems, achieve strong selectivity, less resistance to harmful organisms, and significant control effects

Active Publication Date: 2019-02-01
福建省农业科学院农业生物资源研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, some bio-control actinomycetes with independent intellectual property rights have been reported, but the resources of highly effective bio-control actinomycetes for tomato soil-borne diseases are still very limited

Method used

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  • Streptomyces diastaticus and applications thereof
  • Streptomyces diastaticus and applications thereof
  • Streptomyces diastaticus and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment example 1

[0022] Implementation Case 1: Isolation and Identification of Strain FJAT-31547

[0023] (1) Isolation of bacterial strains: collect soil samples from the tomato planting field in Dongyang Village, Putian, Fujian, weigh 10 g of the soil samples, and press 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 and 10 -6 Perform serial gradient dilutions, and obtain 200 μL of the diluted solution and spread it on Gao’s No. 1 medium (soluble starch 20 g, KNO 3 1g, NaCl 0.5g, K 2 HPO 4 0.5g, MgSO 4 ·7H 2 O 0.5g, FeSO 4 ·7H 2 (00.01g, constant volume to 1L with distilled water, pH 7.3, 121°C, sterilized for 30min), 30°C, cultivated for 7d; the bacterial strain obtained from the culture was re-streaked on the Gaoshi No. 1 medium medium, and placed at 30 Cultivate at ℃ for 7 days, and name the strain obtained from the culture as strain FJAT-31547.

[0024] (2) Morphological identification of the strain: the strain FJAT-31547 was inoculated on Gao's No. 1 medium, cultured at 30°C for 7 days, t...

Embodiment example 2

[0047] Implementation Case 2: Determination of Inhibition Spectrum of Bacterial Strain FJAT-31547

[0048] (1) Antibacterial effect of strain FJAT-31547 on 10 kinds of pathogenic fungi

[0049] Pathogenic fungal strains for testing: 10 kinds of pathogenic fungi, provided by the Institute of Agricultural Biological Resources, Fujian Academy of Agricultural Sciences. See Table 3 below for specific strain information.

[0050] Medium formula: potato sucrose solid medium: potato 200.0g, sucrose 20.0g, agar 18.0g, distilled water to 1L, pH 7.2; semi-solid potato sucrose medium: potato 200.0g, sucrose 20.0g, agar 9g, Distilled water to 1L; potato sucrose liquid medium: 200.0g potato, 20.0g sucrose, distilled water to 1L, pH 7.2; the above medium is used for the cultivation of pathogenic fungi. Gaoshi No. 1 liquid medium for Streptomyces amylase FJAT-31547: soluble starch 20g, sodium chloride 0.5g, ferrous sulfate 0.01g, potassium nitrate 1g, dipotassium hydrogen phosphate 0.5g, ma...

Embodiment example 3

[0065] Implementation case 3: Control effect of strain FJAT-31547 on tomato soil-borne diseases

[0066] (1) Control effect of strain FJAT-31547 on tomato bacterial wilt

[0067] Tomato varieties for testing: "Nongke 180" tomato seeds purchased from Fujian Nongke Agricultural Variety Development Co., Ltd.

[0068]Bacterial suspension preparation: After activating the strain FJAT-31547 on Gaoshi No. 1 solid medium, pick a single colony and put it in Gaoshi No. 1 liquid medium, shake it at 30°C and 170r / min for 7 days, and culture The resulting bacterial solution was diluted with sterile water to make a concentration of 10 7 The bacterial suspension of CFU / mL is ready for use; the existing recognized standard strain GMI1000 of R. Triphenylazole 0.05g, distilled water to 1L, pH value 7.2) after activation, inoculated in SPA liquid medium (sucrose 20g, peptone 5g, KH2PO4 0.5g, MgSO40.025g, distilled water to 1L, pH value 7.2), cultured at 170r / min, 30°C for 48h, and diluted the...

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Abstract

The present invention provides Streptomyces diastaticus and applications thereof, wherein the Streptomyces diastaticus is named Streptomyces diastaticus FJAT-31547, is preserved in the China General Microbiological Culture Collection Center (CGMCC) in March 22, 2018, and has a preservation number of CGMCC No.15479, wherein the address is Institute of Microbiology, Chinese Academy of Sciences, 3# Court No.1, Beichen West Road, Chaoyang District, Beijing, the 16S rRNA sequence of the strain is represented by SEQ ID NO.1, and the strain can be used for preventing and controlling tomato soil bornefungal and bacterial wilt. According to the present invention, the fermentation product of the strain can inhibit tomato wilt caused by Fusarium oxysporum and tomato bacterial wilt caused by Ralstonia solanacearum, has advantages of obvious prevention and control effect and strong selectivity, can provide important guidance significance in the screening and development of broad-spectrum antibacterial active substances from microbial resources in the future, and further can provide strain resources for the biological control of soil borne fungal and bacterial diseases.

Description

【Technical field】 [0001] The invention relates to the technical field of microorganisms, in particular to a Streptomyces amylase and application thereof. 【Background technique】 [0002] With the extensive and long-term application of chemical pesticides, the problem of pest resistance has become increasingly prominent, which has brought hidden dangers to human health and environmental safety. Biological control has become a research hotspot because of its high efficiency, strong selectivity, less resistance to harmful organisms, and low toxicity to humans and animals. Actinomycetes are the first microorganisms to be found to have biocontrol effects, among which Streptomyces, Nocardia, Actinoplanes, Micromonospora, etc. are mainly used in biocontrol. Actinomycetes biological control preparations have the advantages of non-toxicity, no residue, environmental friendliness, and long-lasting effect of disease prevention, etc., and have broad application prospects in biological c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P1/00A01P3/00C12R1/465
CPCA01N63/00A01N63/10C12N1/205C12R2001/465
Inventor 郑雪芳刘波朱育菁王阶平张海峰陈德局
Owner 福建省农业科学院农业生物资源研究所