Circular RNA marker hsa_circ_0001788 and application thereof

Abstract

The invention establishes a circRNA expression profile for a patient with craniosynostosis, provides, provides serum circRNA marker hsa_circ_0001788 for the patient with craniosynostosis, discloses diagnostic value of hsa_circ_0001788 of serum for craniosynostosis, applies the marker herein to prepare a diagnostic kit for the patient with craniosynostosis, and provides supporting for clinical early discovery and treatment of the patient with craniosynostosis. Serum circRNA has good stability, degradation difficulty and the other advantage, has high value in auxiliary diagnosis, and is easy toclinically popularize and use. A circRNA chip is used herein to carry out detecting to obtain disease-specific and abnormally-expressed serum circRNA expression profiles; verifying is performed by means of qRT-PCR; a strict design and evaluation system is provided. It is discovered for the first time that the marker hsa_circ_0001788 in serum is an important biological detection index which causesminimal invasion and has low cost and which is applicable to clinical detection; the marker hsa_circ_0001788 is important to the clinical early diagnosis and differential diagnosis of craniosynostosis; a new idea is provided for the diagnosis of craniosynostosis in molecular level, and it is expected that early diagnostic rate of craniosynostosis is increased.

Description

technical field

[0001] The invention belongs to the fields of biotechnology, preventive medicine and clinical medicine, and discloses a circRNA marker related to auxiliary diagnosis of craniosynostosis and its application, specifically, the circular RNA marker hsa_circ_0001788 and its application. Background technique

[0002] Craniosynostosis is a common craniofacial deformity with an incidence rate of about 1 / 2500, ranking second in the incidence rate of congenital craniofacial bone deformities. It is characterized by premature fusion of one or more sutures before brain development is complete, resulting in restricted development of the skull, brain, face, and central nervous system. The disease will have a serious impact on the appearance of the patient. In some cases, the narrow cranial cavity can hinder the development of brain tissue, causing mental and motor development to lag behind. At present, the diagnosis of craniosynostosis is mainly based on abnormal head shap...

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