Method for determining AA content in mouse cerebrospinal fluid by using RhB@MOF-5 composite material

A technology for composite materials and cerebrospinal fluid, applied in measuring devices, analytical materials, material excitation analysis, etc., can solve undiscovered problems and achieve the effects of reduced deprotonation, high sensitivity, and high selectivity

Active Publication Date: 2019-02-01
QUFU NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, there is no report on the determination of AA (ascorbic acid, vitamin C) content using dye@MOFs composite materials at home and abroad.

Method used

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  • Method for determining AA content in mouse cerebrospinal fluid by using RhB@MOF-5 composite material
  • Method for determining AA content in mouse cerebrospinal fluid by using RhB@MOF-5 composite material
  • Method for determining AA content in mouse cerebrospinal fluid by using RhB@MOF-5 composite material

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Embodiment 1

[0040] The preparation process of the RhB@MOF-5 composite material of the present invention is as follows:

[0041] Accurately weigh 2.10g zinc nitrate hexahydrate (7.00mmol), 0.42g terephthalic acid (2.50mmol) and 0.12g rhodamine B (RhB) (0.25mmol) in a 20mL screw cap glass vial, add 12.0mLN , N-dimethylformamide (DMF) and magnetically stirred until dissolved. The glass bottle was heated and reacted at 120°C for 24 hours, and the crystallization process was kept in a static state. Cool to room temperature after the reaction, centrifuge to obtain pink crystals, wash with DMF until no RhB molecules are detected in the supernatant (monitored by a fluorescence spectrometer). The finally obtained crystals were dried at 250°C and stored in the dark at 4°C.

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Abstract

The invention belongs to the technical field of metalorganic framework composite materials, and particularly relates to a method for determining AA content in the mouse cerebrospinal fluid by using the RhB@MOF5 composite material. The method comprising the following steps: adding Fe3 to a RhB@MOF5 composite aqueous solution to form a RhB@MOF5/Fe3 composite aqueous solution, using the internal rateeffect and photoinduced electron transfer between Fe3 and RhB@MOF5, the quenching the fluorescence of the RhB@MOF5 composite material; then adding AA, wherein the AA reduces Fe3 to Fe2, which restores the fluorescence of RhB@MOF5 composite material, and determining its fluorescence spectrum change. The method of the invention is characterized in that the AA content in the cerebrospinal fluid of the mouse is highly selective and sensitive.

Description

technical field [0001] The invention belongs to the technical field of metal-organic framework composite materials, and in particular relates to a method for measuring AA content in mouse cerebrospinal fluid by using RhB@MOF-5 composite materials. Background technique [0002] Metal-organic frameworks, or MOFs for short, are organic-inorganic hybrid materials with intramolecular pores formed by the self-assembly of organic ligands and metal ions or clusters through coordination bonds. In MOFs, the arrangement of organic ligands and metal ions or clusters has obvious directionality, which can form different framework pore structures, thus exhibiting different adsorption properties, optical properties, electromagnetic properties, etc. MOFs present great development potential and attractive development prospects in modern materials science. However, the application of MOFs materials in the field of analytical chemical sensing lags far behind other fields. [0003] At present,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428G01N2021/6417G01N2021/6432
Inventor 夏莲郭兰窦娅楠
Owner QUFU NORMAL UNIV
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