A method for removing flesh from bone specimens
A bone and specimen technology, applied in the field of specimen preparation, can solve the problems of cumbersome production process, danger of strong acid and strong alkali, and limited application range, etc., and achieve the effect of simple flesh removal method, time saving and convenient use
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Embodiment 1
[0043] Example 1 A method for removing flesh from bone specimens
[0044] A method for removing flesh from a bone specimen, comprising the following steps:
[0045] (1) Use a scalpel to remove 85% to 90% of the soft tissue of the dead animal, keep the bone part, and then divide it into limbs, skull, thorax, lumbar spine and hip bone parts through the bones, and pack them in mesh bags;
[0046] (2) Prepare the solution: Prepare the solution according to the mass ratio of Novozymes protease: fur degreasing agent: water = 40:1:2000; put the solution in a constant temperature water bath at 60°C for 4 hours until the solution temperature is constant; among them, The concentration of Novozymes protease is 2%, and the concentration of fur degreasing agent is 0.05%;
[0047] (3) Put the encapsulated bone in step (1) into the above solution so that the solution just submerges the bone. It should be noted that the skull is relatively thin and does not take too long, so the skull is pla...
Embodiment 2
[0049] Example 2 A method for removing flesh from bone specimens
[0050] A method for removing flesh from a bone specimen, comprising the following steps:
[0051] (1) Use a scalpel to remove 85% to 90% of the soft tissue of the dead animal, keep the bone part, and then divide it into limbs, skull, thorax, lumbar spine and hip bone parts through the bones, and pack them in mesh bags;
[0052] (2) Prepare the solution: Prepare the solution according to the mass ratio of alkaline protease: alkaline degreasing agent: water = 30:1:2050; put the solution in a constant temperature water bath at 55°C for 5 hours until the solution temperature is constant; among them, The concentration of alkaline protease is 1.5%, and the concentration of alkaline degreasing agent is 0.1%; Alkaline protease and alkaline degreasing agent are commercially available;
[0053] (3) Put the encapsulated bone in step (1) into the above solution so that the solution just submerges the bone. It should be no...
Embodiment 3
[0055] Example 3 A method for removing flesh from bone specimens
[0056] A method for removing flesh from a bone specimen, comprising the following steps:
[0057] (1) Use a scalpel to remove 85% to 90% of the soft tissue of the dead animal, keep the bone part, and then divide it into limbs, skull, thorax, lumbar spine and hip bone parts through the bones, and pack them in mesh bags;
[0058] (2) Prepare the solution: Prepare the solution according to the mass ratio of neutral protease: solvent degreasing agent: water = 45:2:2000; put the solution in a constant temperature water bath at 60°C for 5 hours until the solution temperature is constant; among them, The concentration of neutral protease is 2.5%, and the concentration of solvent degreasing agent is 0.08%; Neutral protease and solvent degreasing agent are commercially available;
[0059] (3) Put the encapsulated bone in step (1) into the above solution so that the solution just submerges the bone. It should be noted t...
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