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A method for removing flesh from bone specimens

A bone and specimen technology, applied in the field of specimen preparation, can solve the problems of cumbersome production process, danger of strong acid and strong alkali, and limited application range, etc., and achieve the effect of simple flesh removal method, time saving and convenient use

Active Publication Date: 2021-03-30
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Animal bone specimens and bone specimen production techniques are also found in some textbooks and magazine articles. Traditional bone specimen production methods generally include: flesh removal, bone marrow removal, corrosion, bleaching and other steps. The production process is cumbersome, especially the flesh removal step. It is large and requires the operator to have strong professional knowledge and operation skills, otherwise it will cause bone damage and the production cycle will be long
[0004] Among the existing bone fleshing methods, most of them use the boiling method, the strong acid and strong alkali method, and the insect eating method to remove the flesh. These methods have highlighted many shortcomings in practical applications, such as the boiling method takes too long and is easy to Damage to bones, strong acid and strong alkali have certain dangers, and the method of phagocytosis is more suitable for small animal bones
Moreover, the bone structure of each part of the animal body is different, and the composition of soft tissue is not limited to muscle, etc., but the current methods are not universally applicable, and the bones treated by the above methods are often damaged, and the flesh is not clean
In addition, the traditional bone production method is mainly suitable for relatively large animals, which has low practicality and limited application range, so it cannot be widely used by people.

Method used

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  • A method for removing flesh from bone specimens

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1 A method for removing flesh from bone specimens

[0044] A method for removing flesh from a bone specimen, comprising the following steps:

[0045] (1) Use a scalpel to remove 85% to 90% of the soft tissue of the dead animal, keep the bone part, and then divide it into limbs, skull, thorax, lumbar spine and hip bone parts through the bones, and pack them in mesh bags;

[0046] (2) Prepare the solution: Prepare the solution according to the mass ratio of Novozymes protease: fur degreasing agent: water = 40:1:2000; put the solution in a constant temperature water bath at 60°C for 4 hours until the solution temperature is constant; among them, The concentration of Novozymes protease is 2%, and the concentration of fur degreasing agent is 0.05%;

[0047] (3) Put the encapsulated bone in step (1) into the above solution so that the solution just submerges the bone. It should be noted that the skull is relatively thin and does not take too long, so the skull is pla...

Embodiment 2

[0049] Example 2 A method for removing flesh from bone specimens

[0050] A method for removing flesh from a bone specimen, comprising the following steps:

[0051] (1) Use a scalpel to remove 85% to 90% of the soft tissue of the dead animal, keep the bone part, and then divide it into limbs, skull, thorax, lumbar spine and hip bone parts through the bones, and pack them in mesh bags;

[0052] (2) Prepare the solution: Prepare the solution according to the mass ratio of alkaline protease: alkaline degreasing agent: water = 30:1:2050; put the solution in a constant temperature water bath at 55°C for 5 hours until the solution temperature is constant; among them, The concentration of alkaline protease is 1.5%, and the concentration of alkaline degreasing agent is 0.1%; Alkaline protease and alkaline degreasing agent are commercially available;

[0053] (3) Put the encapsulated bone in step (1) into the above solution so that the solution just submerges the bone. It should be no...

Embodiment 3

[0055] Example 3 A method for removing flesh from bone specimens

[0056] A method for removing flesh from a bone specimen, comprising the following steps:

[0057] (1) Use a scalpel to remove 85% to 90% of the soft tissue of the dead animal, keep the bone part, and then divide it into limbs, skull, thorax, lumbar spine and hip bone parts through the bones, and pack them in mesh bags;

[0058] (2) Prepare the solution: Prepare the solution according to the mass ratio of neutral protease: solvent degreasing agent: water = 45:2:2000; put the solution in a constant temperature water bath at 60°C for 5 hours until the solution temperature is constant; among them, The concentration of neutral protease is 2.5%, and the concentration of solvent degreasing agent is 0.08%; Neutral protease and solvent degreasing agent are commercially available;

[0059] (3) Put the encapsulated bone in step (1) into the above solution so that the solution just submerges the bone. It should be noted t...

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PUM

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Abstract

The invention discloses a method of removing meat from a bone specimen. The method comprises the following steps: 85%-90% of soft tissues are removed from an animal carcass, and a bone part is kept; the bones are then divided to limb, skull, thorax, lumbar vertebra and hipbone parts, and the parts are packaged respectively; after a mixed solution is prepared by protease, a degreasing agent and water with a mass ratio of 20-50 to 1-2 to 2000-2100, and thermostatic waterbath is carried out; the well-packaged bones are put in the mixed solution respectively, the bones are just immersed by the mixed solution and are soaked for 4 to 5 h, the soft tissues are washed out by high pressure water, and the impurities on the bones are removed; and the bones are then put in the mixed solution again andare soaked until no impurities exist on the surfaces of the bones, the bones are taken out, the soft tissues are washed out by high pressure water, and then, the bones are washed clean by using a detergent. The processed bones are clean and complete, damages of the bones are avoided, the meat removal method is simple, the meat removal time is shortened in a condition of maintaining the integrityof the bones, and the application potential is great.

Description

technical field [0001] The invention belongs to the technical field of specimen preparation. Specifically, it relates to a method for removing flesh from a bone specimen. Background technique [0002] Animal specimens play a particularly important role in biological research, teaching, and popular science work. Skeleton specimens are the main materials for studying the morphology and structure of animal bones and their relationship with other organs. Animal bone specimens not only have the function of display, but also have traditional It also plays an extremely important role in morphological taxonomy and archaeology. With the development of genetics, researchers often need to classify from both morphological and genetic aspects, which requires bone specimens of various animals for data support. Therefore, the production of animal bone specimens is particularly important. [0003] Animal bone specimens and bone specimen production techniques are also found in some textboo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G09B23/36A01N1/00
CPCA01N1/00G09B23/36
Inventor 剡海阔王兴贤肖丹媚邵洋洋陈路漫范小龙许丹邓衔柏
Owner SOUTH CHINA AGRI UNIV
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