Application of lobetyolin similar compound in preparing drug for treating arrhythmia
A technology of Codonopsis glucoside and compound is applied in the application field of Codonopsis glucoside-like compound in the preparation of drugs for treating arrhythmia, and can solve the problems of unclear material basis of pharmacological activity, and unrevealed mechanism of action of a single effective chemical component in Codonopsis pilosula. , to achieve broad market application prospects, simple and reliable operation
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Embodiment 1
[0030] The extraction and separation method of embodiment 1 styoside analogue compound (compound Lobeyolinin):
[0031] 8 g of the commercially available Codonopsis glucoside extract (Dausifu, Nanjing) extract was dissolved in 40 mL of pure water by ultrasonic, and the Swiss Buchi pump control system (Pump Manager C-615, PumpModule C-601) were separated and prepared, eluted with pure water, 20%, 40%, 60%, 80%, and 100% ethanol respectively for 3 column volumes, collected and concentrated respectively. The 40% eluate was then eluted with 20%, 22%, 24%, 26%, 28%, 30%, and 100% ethanol for 3 column volumes, and the concentrated solution was collected and concentrated respectively. According to the liquid chromatogram (see attached figure 1 ), the compound Lobetyolinin is mainly concentrated in 22%, 24%, 26% elution part, so 22%, 24%, 26% elution part is then equipped with a semi-preparative column (ZOBARX SB-C18, 21.2×250mm, Agilent) purification, water (A)-acetonitrile (B) is ...
Embodiment 2
[0036] Experiments related to the antiarrhythmic activity of the compounds tangshenoside and lobeyolinin in Example 2:
[0037]2.1. Drugs, reagents, animals and instruments:
[0038] Lobeyolinin, the compound isolated above; Cmlc2-GFP transgenic zebrafish; N-Phenylthiourea (PTU) and Tricaine; Terfenadine; Leica 3000B fluorescence inverted microscope; IX-100F zebrafish ECG recording system.
[0039] 2.2. Zebrafish Arrhythmia Model Establishment
[0040] Cmlc2-GFP transgenic zebrafish is a transgenic line with heart-specific marker green fluorescent protein. Several pairs of heterozygous Cmlc2-GFP transgenic zebrafish females and males were taken, and the embryos were harvested after mating and cultured at a constant temperature of 28°C. After 24 hours of development, the embryos with fluorescence were selected under a fluorescence microscope, and the membranes were ruptured and grouped. Each group had 8-10 embryos, added PTU to inhibit the formation of stains, and cultured at...
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