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Application of lobetyolin similar compound in preparing drug for treating arrhythmia

A technology of Codonopsis glucoside and compound is applied in the application field of Codonopsis glucoside-like compound in the preparation of drugs for treating arrhythmia, and can solve the problems of unclear material basis of pharmacological activity, and unrevealed mechanism of action of a single effective chemical component in Codonopsis pilosula. , to achieve broad market application prospects, simple and reliable operation

Active Publication Date: 2019-02-05
SHANDONG BUCHANG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] But so far, the extraction and pharmacological research of the chemical components of Codonopsis Codonopsis plants at home and abroad are mainly concentrated on polysaccharides, saponins and other effective parts, and the mechanism of action of the single effective chemical components in Codonopsis pilosula has not yet been revealed, and related The material basis of its pharmacological activity is still unclear
So far, no report has been found about the effect of tangshenoside analogues on the treatment of arrhythmia

Method used

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  • Application of lobetyolin similar compound in preparing drug for treating arrhythmia
  • Application of lobetyolin similar compound in preparing drug for treating arrhythmia
  • Application of lobetyolin similar compound in preparing drug for treating arrhythmia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The extraction and separation method of embodiment 1 styoside analogue compound (compound Lobeyolinin):

[0031] 8 g of the commercially available Codonopsis glucoside extract (Dausifu, Nanjing) extract was dissolved in 40 mL of pure water by ultrasonic, and the Swiss Buchi pump control system (Pump Manager C-615, PumpModule C-601) were separated and prepared, eluted with pure water, 20%, 40%, 60%, 80%, and 100% ethanol respectively for 3 column volumes, collected and concentrated respectively. The 40% eluate was then eluted with 20%, 22%, 24%, 26%, 28%, 30%, and 100% ethanol for 3 column volumes, and the concentrated solution was collected and concentrated respectively. According to the liquid chromatogram (see attached figure 1 ), the compound Lobetyolinin is mainly concentrated in 22%, 24%, 26% elution part, so 22%, 24%, 26% elution part is then equipped with a semi-preparative column (ZOBARX SB-C18, 21.2×250mm, Agilent) purification, water (A)-acetonitrile (B) is ...

Embodiment 2

[0036] Experiments related to the antiarrhythmic activity of the compounds tangshenoside and lobeyolinin in Example 2:

[0037]2.1. Drugs, reagents, animals and instruments:

[0038] Lobeyolinin, the compound isolated above; Cmlc2-GFP transgenic zebrafish; N-Phenylthiourea (PTU) and Tricaine; Terfenadine; Leica 3000B fluorescence inverted microscope; IX-100F zebrafish ECG recording system.

[0039] 2.2. Zebrafish Arrhythmia Model Establishment

[0040] Cmlc2-GFP transgenic zebrafish is a transgenic line with heart-specific marker green fluorescent protein. Several pairs of heterozygous Cmlc2-GFP transgenic zebrafish females and males were taken, and the embryos were harvested after mating and cultured at a constant temperature of 28°C. After 24 hours of development, the embryos with fluorescence were selected under a fluorescence microscope, and the membranes were ruptured and grouped. Each group had 8-10 embryos, added PTU to inhibit the formation of stains, and cultured at...

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Abstract

The invention relates to application of a lobetyolin similar compound in preparing a drug for treating arrhythmia. The invention provides an efficient and stable separation and purification method ofthe lobetyolin similar compound. By adopting the method, the lobetyolin similar compound with relatively high content and purity is obtained. The preparation method is simple and reliable to operate and can be applied to technological mass production. Proved with pharmacological experiments, the compound has the effects of myocardial preservation and arrhythmia resistance, can be used as a drug for clinically treating the arrhythmia and has a wide market application prospect.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine, and in particular relates to the application of a tangshenoside analog compound in the preparation of drugs for treating arrhythmia. Background technique [0002] Arrhythmia is one of the most common diseases of the cardiovascular system and the main cause of sudden cardiac death. Arrhythmia can occur independently or be secondary to various cardiovascular diseases. Patients with heart failure are often accompanied by arrhythmia. Arrhythmia is a complex disease involving multiple links and factors. The existing clinical treatment methods are mainly surgery and drugs. Antiarrhythmic drugs and anticoagulant drugs are mainly used in drug treatment. Among them, antiarrhythmic drugs are divided into four categories: I, II, III, and IV, mainly various ion channel blockers and β-adrenoceptor blockers. However, commonly used antiarrhythmic drugs are accompanied by arrhythmogenic side effects...

Claims

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Application Information

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IPC IPC(8): A61K31/7032A61P9/06A61P9/00C07H15/04C07H1/08
CPCA61K31/7032A61P9/00A61P9/06C07H1/08C07H15/04
Inventor 王毅赵步长赵涛王一民王勇吴建涛谢伟陆世海千珂史洋
Owner SHANDONG BUCHANG PHARMA
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