Method for extracting apple polyphenol from apple
An apple polyphenol and apple technology, which is applied in pharmaceutical formulations, preparations for skin care, medical preparations containing active ingredients, etc., can solve the problems of low yield of apple polyphenols, and achieve a short extraction cycle, high product purity, and high technology. Simple process effect
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Embodiment 1
[0024] A method for extracting apple polyphenols from apples, comprising the following steps:
[0025] (1) Select 6-8 mature fresh apples, smash the apples, and use pomace as raw material;
[0026] (2) Put pomace in continuous countercurrent ultrasonic equipment, add a 60v% ethanol solution with a solid-liquid ratio of 1:28, adjust the pH value between 4.5, add an antioxidant in the aqueous solution, the antioxidant is ascorbic acid, and the added The amount is 0.6% of the total weight of pomace. After ultrasonic treatment for a period of time, filter to obtain the ultrasonic crude extract; the ultrasonic conditions are: ultrasonic frequency 40KHz, ultrasonic power is 180W, ultrasonic treatment time is 45min, and the working temperature is 45- 50°C.
[0027] (3) Pass the ultrasonic crude extract through the DA201 macroporous resin column, the adsorption temperature is 48-52°C, the speed of the crude extract on the column is 0.6-0.8BV / h, first wash the column with 60v% ethanol...
Embodiment 2
[0031] A method for extracting apple polyphenols from apples, comprising the following steps:
[0032] (1) Select 6-8 mature fresh apples, smash the apples, and use pomace as raw material;
[0033] (2) Put pomace into continuous countercurrent ultrasonic equipment, add a 70v% ethanol solution with a solid-liquid ratio of 1:20, adjust the pH value between 4, add an antioxidant in the aqueous solution, the antioxidant is ascorbic acid, and the added The amount is 0.3% of the total weight of pomace, and after ultrasonic treatment for a period of time, filter to obtain the ultrasonic crude extract; the ultrasonic conditions are: ultrasonic frequency 50KHz, ultrasonic power is 150W, ultrasonic treatment time is 60min, and the working temperature is 50- 55°C.
[0034] (3) Pass the ultrasonic crude extract through a DM301 macroporous resin column, the adsorption temperature is 45-48°C, the speed of the crude extract on the column is 0.6-0.8BV / h, first wash the column with 50v% ethan...
Embodiment 3
[0038] A method for extracting apple polyphenols from apples, comprising the following steps:
[0039] (1) Select 6-8 ripe fresh apples, smash the apples, and use pomace as raw material;
[0040] (2) Put pomace into continuous countercurrent ultrasonic equipment, add a 50v% ethanol solution with a solid-liquid ratio of 1:33, adjust the pH value between 5, add an antioxidant in the aqueous solution, the antioxidant is ascorbic acid, and the added The amount is 1% of the total weight of pomace. After ultrasonic treatment for a period of time, filter to obtain the ultrasonic crude extract; the ultrasonic conditions are: ultrasonic frequency 30KHz, ultrasonic power is 200W, ultrasonic treatment time is 30min, and the working temperature is 40- 45°C.
[0041] (3) Pass the ultrasonic crude extract through the DA201 macroporous resin column, the adsorption temperature is 52-55°C, the speed of the crude extract on the column is 0.6-0.8BV / h, first wash the column with 75v% ethanol sol...
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