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Application of dammarane triterpene saponins in anti-hypoxia

A triterpene saponin and dammarane-type technology, which is applied in the field of application of dammarane-type triterpene saponins in anti-hypoxia, can solve problems such as unclear anti-hypoxia activity, reduce brain tissue damage, improve cell Vitality effect

Active Publication Date: 2020-06-30
ACADEMY OF MILITARY MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The total saponins of Gynostemma pentaphyllum are complexes of many types of compounds. At present, it is not clear which bioactive components play an anti-hypoxic activity

Method used

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  • Application of dammarane triterpene saponins in anti-hypoxia
  • Application of dammarane triterpene saponins in anti-hypoxia
  • Application of dammarane triterpene saponins in anti-hypoxia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] In vitro experiments confirmed that the compound has anti-hypoxic activity

[0019] 1. Cell culture

[0020] Rat adrenal pheochromocytoma PC12 cells were cryopreserved in our laboratory. Cells were cultured in RPMI-1640 medium (Gibco) containing 10% fetal bovine serum, 100U / ml penicillin and 100μg / ml streptomycin, at 5% CO 2 , Cultivate at 37°C under saturated humidity, and take logarithmic growth phase cells for experiment.

[0021] 2. Cell viability test

[0022] Cell proliferation / toxicity assay (Cell Counting Kit-8, CCK-8) kit (Dojindo, Japan) was used to detect cell viability. PC12 cells in logarithmic growth phase were seeded in 96-well plates with 4000 cells per well. The cells were divided into a control group and a drug treatment group, and the concentration of each monomer compound was 3,30 μM, respectively. Hypoxia group cells are placed in O 2 In a hypoxic box with a concentration of 0.1%. After the treatment group reached the time point, 10μl of CCK-8 solution ...

Embodiment 2

[0026] In vivo experiments confirmed that the compound has anti-hypoxic activity

[0027] 1. Mice and simulated altitude hypoxia exposure

[0028] The experimental animals were C57BL / 6 mice for qualified experimental animals, male, purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd. Animals are kept in a constant temperature (23±2°C), constant humidity animal room, which circulates day and night for 12 / 12h every day, and they can eat and drink freely. The low-pressure hypoxic animal experiment cabin that simulates the plateau environment was purchased from Guizhou Fenglei Aviation Ordnance Co., Ltd., model: DYC-DWI. The cabin is connected with two air pumps, which can continuously pump out the gas in the cabin to simulate a low-pressure and oxygen-deficient environment at a certain altitude; at the same time, it is connected with an electronic display and an oxygen sensor probe (real-time display of the cabin Altitude and oxygen concentration). Twenty m...

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Abstract

The invention discloses compounds with an anti-hypoxia effect, and belongs to the technical field of biology. The invention finds that the dammarane-type triterpenoid saponins separated and purified from gynostemma pentaphyllum have novel activity, namely anti-hypoxia activity for the first time. The invention provides five dammarane-type triterpenoid saponins separated from the gynostemma pentaphyllum, and cell experiments and mouse experiments show that the dammarane-type triterpenoid saponins have obvious anti-hypoxia activity. Candidate compounds for preparing novel anti-hypoxia medicinesare provided, and a significance in development and utilization of Chinese herbs such as the gynostemma pentaphyllum is important.

Description

Technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to the application of dammarane-type triterpene saponins in anti-hypoxia. Background technique [0002] Hypoxia refers to the severe reduction of oxygen in the environment where cells, tissues or biological organisms are located. Hypoxia under pathological conditions can cause tissue damage and participate in the progression and deterioration of many diseases, such as myocardial infarction and cerebral infarction. Environmental hypoxia (high altitude areas or deep water diving) can reduce arterial blood oxygen saturation and induce hypoxia of the body tissues throughout the body, and induce hypoxic diseases. Therefore, physical (such as mild hypoxic pretreatment) or chemical (drug administration) discovery of effective intervention is essential to prevent hypoxia-related diseases. [0003] Model animals and clinical intervention trials have proved that natural compounds may play a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J17/00A61P39/00
CPCA61P39/00C07J17/005
Inventor 赵名朱玲玲成祥赵彤范明周延召韩莹李改芬
Owner ACADEMY OF MILITARY MEDICAL SCI
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