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Agaricus blazei murill polypeptide as well as preparation method and application of polypeptide thereof

A technology of Agaricus blazei and velvet, which is applied in the field of natural active products, can solve the problems of poor effect, drug use for people with memory loss, complex ingredients, etc., and achieve the effect of improving learning ability and improving memory impairment of animals

Inactive Publication Date: 2019-02-15
BEIHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these chemical drugs have more or less unacceptable adverse reactions, and are currently only used for the treatment of brain damage and the resulting neurological loss, memory and intellectual impairment, and cannot be used for the general population of memory loss.
[0005] At present, there are also some brain-enhancing and brain-replenishing products on the domestic and foreign markets, but most of them have the defects of poor effect or complicated ingredients.

Method used

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  • Agaricus blazei murill polypeptide as well as preparation method and application of polypeptide thereof
  • Agaricus blazei murill polypeptide as well as preparation method and application of polypeptide thereof
  • Agaricus blazei murill polypeptide as well as preparation method and application of polypeptide thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A Agaricus blazei polypeptide is prepared by the following method:

[0030] 1. Extraction.

[0031] Take fresh Agaricus blazei fruiting body, after washing, add water according to the amount of 1g fruiting body: 1mL water, place on ice for homogenization; then ultrasonic extraction, ultrasonic power is 200w, time is 1hr, centrifuged at 4000r / min and taken out supernatant; take part of the supernatant, recover the solvent, and obtain the water extract.

[0032] 2. Salting out.

[0033] Add solid ammonium sulfate to the above supernatant for salting out and precipitation, so that the saturation of ammonium sulfate is 80%; centrifuge at 4000r / min after 24 hours of precipitation, take the precipitate, and dialyze it with a dialysis bag with a molecular weight cut-off of 3.5kD for 8-12hr. The dialyzate obtained after freeze-drying;

[0034] 3. Purification.

[0035] 3.1 Ion exchange chromatography separation.

[0036] The above-mentioned dialyzate was separated with a D...

experiment example 1

[0040] Establish the D-gal mouse model of learning and memory impairment and test the activity of the test product.

[0041] 1. Method.

[0042] 1.1 Grouping and administration.

[0043] The ICR mice were randomly divided into blank group, model group, positive drug group (piracetam) and administration group, wherein the administration group included: water extract group, sediment group, dialyzate group, 130-200min fraction Group, 500-560min distillation group, crude extract group, 50-90min distillation group, Agaricus blazei polypeptide group, 10 rats in each group.

[0044]Except for the blank group, the mice in other groups were injected with D-gal 300mg / kg at a fixed time every day, the mice in the blank group were injected with the same dose of normal saline, and the positive control group was administered 800mg / kg of piracetam every day at the same time. The drug group was given 800 mg / kg test drug by intragastric administration every day. In order to carry out the ex...

experiment example 2

[0083] Serum index detection.

[0084] Serum sample preparation: After the above behavioral experiment, that is, 24 hours after the last administration, blood was collected from the orbit, and the serum was separated, stored at 4°C, centrifuged at 5000rpm / min for 10min, and the serum was separated and stored at -80°C for testing. Refer to the kit instructions to detect the content of CAT, T-AOC, ROS and MDA in serum.

[0085] (1) Detection of CAT in serum: The detection steps are determined according to the instructions of the kit (Nanjing Jiancheng Institute of Biological Products, A007-1), as shown in the table below.

[0086] (2) Detection of T-AOC in serum: The detection steps were determined according to the instructions of the kit (Nanjing Jiancheng Institute of Biological Products, A015-2), as shown in the table below.

[0087] (3) Detection of ROS in serum: The detection steps are determined according to the instructions of the kit (Nanjing Jiancheng Institute of Biol...

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Abstract

The invention relates to agaricus blazei murill polypeptide as well as a preparation method and an application thereof, and belongs to the technical field of natural active products. The preparation method comprises the following steps: extraction: taking an agaricus blazei murill fruiting body, adding water for homogenization, performing ultrasound extraction and centrifugation, and obtaining supernate, salting out: adding ammonium sulfate into the supernate for salting out and sediment, and taking a sediment for dialysis to form a dialysate, and purification: separating the dialysate throughan ion-exchange column to form a crude extract, and purifying the crude extract through a sephadex column to form the agaricus blazei murill polypeptide. The agaricus blazei murill polypeptide prepared according to the method has obvious bioactivity and can improve animal dysmnesia and learning capacity.

Description

technical field [0001] The invention relates to the technical field of natural active products, in particular to a Agaricus blazei polypeptide and its preparation method and application. Background technique [0002] As we age, our brain function declines, and our responsiveness and memory also decline. Modern people's life is fast-paced, and it is common to work overtime and stay up late, which greatly reduces their sleep time and is in a state of long-term sleep deprivation. Long-term sleep deprivation will have some health risks to the health of the body, such as affecting the function of the brain and reducing responsiveness, memory and judgment. [0003] For middle-aged and elderly people, especially those with diseases such as hypertension and cerebral arteriosclerosis, their memory ability fluctuates significantly. There are also certain diseases, such as neurasthenia, depression, cerebral arteriosclerosis, chronic sinusitis, etc., which are all common causes of mem...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/36C07K1/30C07K1/34C07K1/18C12G3/05A23L33/18A23F3/34A61K38/01A61P25/00
CPCA23F3/34A23V2002/00A61K38/011A23L33/18A61P25/00C07K1/18C07K1/303C07K1/34C07K1/36C12G3/04A23V2200/30A23V2250/55
Inventor 安丽萍盛瑜冯晴霞杜培革徐广宇韩笑郭笑王曼力赵南晰孙晶波苑广信李洪宇王锰
Owner BEIHUA UNIV
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