Cordyceps militaris culture method capable of realizing high yield of carotenoids

A technology of carotene and culture method, which is applied in the field of liquid submerged fermentation culture of Cordyceps militaris with high yield of carotenoids, can solve the problems of low carotenoid fermentation level, etc., and achieve the effect of improving fermentation level and fermentation yield

Active Publication Date: 2019-02-15
安徽汉韵生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] For this reason, the technical problem to be solved by the present invention is to provide a method for cultivating Cordyceps militaris with high yield of carotenoids, to solve the problem that the overall fermentation level of carotenoids is low in the liquid fermentation process of Cordyceps militaris in the prior art

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  • Cordyceps militaris culture method capable of realizing high yield of carotenoids

Examples

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Embodiment 1

[0021] The method for culturing Cordyceps militaris with high carotenoid production in this embodiment includes activating strains in a strain activation medium, culturing seed liquid in a seed liquid medium, and fermenting in a mycelium fermentation medium Steps for jar mycelium culture.

[0022] The strain activation medium described in this example includes the following components by mass: the above components are taken according to the proportions of 5% potato, 3% molasses, 0.2% humic acid powder, and 0.2% hawthorn; 50 g of potato and 30 g of molasses are taken respectively. , 2g of humic acid powder, 2g of hawthorn (nucleated), add water to make pulp and dilute to 1L, natural pH, sterilize at 121°C for 20min, then pack in test tubes, connect Cordyceps militaris strains under aseptic conditions Put it into a test tube, and carry out activation culture in the dark for 2-3 days at 20°C and rotating speed of 150rpm.

[0023] The seed liquid medium described in this example ...

Embodiment 2

[0026] The method for culturing Cordyceps militaris with high carotenoid production in this embodiment includes activating strains in a strain activation medium, culturing seed liquid in a seed liquid medium, and fermenting in a mycelium fermentation medium Steps for jar mycelium culture.

[0027] The strain activation medium described in this example includes the following components by mass content: the above components are taken according to the proportions of 10% potato, 1% molasses, 0.4% humic acid powder, and 0.1% hawthorn; 100 g of potato and 10 g of molasses are taken respectively. , 4 g of humic acid powder, 1 g of hawthorn (nucleated), add water to make pulp and dilute to 1 L, natural pH, sterilize at 121 ° C for 20 min, then pack in test tubes, and connect Cordyceps militaris strains under aseptic conditions. Put it into a test tube, and carry out activation culture in the dark for 2-3 days at 25°C and rotating speed of 100rpm.

[0028] The seed liquid medium descr...

Embodiment 3

[0031] The method for culturing Cordyceps militaris with high carotenoid production in this embodiment includes activating strains in a strain activation medium, culturing seed liquid in a seed liquid medium, and fermenting in a mycelium fermentation medium Steps for jar mycelium culture.

[0032] The strain activation medium described in this example includes the following components by mass content: the above components are taken according to the proportions of 8% potato, 2% molasses, 0.3% humic acid powder, and 0.15% hawthorn; 80g potato and 20g molasses are respectively taken. , 3g of humic acid powder, 1.5g of hawthorn (nucleated), add water to make pulp and dilute to 1L, natural pH, sterilize at 121°C for 20min, then divide into test tubes, put the Cordyceps militaris strain under aseptic conditions Into the test tube, at 22 ° C, rotating speed of 120 rpm for activation and culture in the dark for 2-3 days.

[0033] The seed liquid medium described in this example inclu...

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Abstract

The invention belongs to the field of cordyceps militaris, and relates to a cordyceps militaris culture method capable of realizing high yield of carotenoids, in particular to a cordyceps militaris submerged fermentation culture method capable of realizing high yield of carotenoids. According to the cordyceps militaris culture method capable of realizing high yield of carotenoids, a strain activation medium, a seed solution medium and a liquid fermentation medium are screened elaborately, molasses, peptone, palm oil, hawthorn fruit, Lu'an tea powder, chitin, humic acid powder, Enteromorpha, zinc-amino acid chelate, water-soluble silicon, KH2PO4 and K2HPO4 are taken as main nutrition raw materials, and fermentation yield of carotenoids from cordyceps militaris is increased effectively; furthermore, vitamin C palmitate is added to the fermentation medium, so that accumulation of carotenoids is induced better, fermentation yield of carotenoids is further increased, and fermentation levelof carotenoids in the cordyceps militaris is increased effectively.

Description

technical field [0001] The invention belongs to the field of Cordyceps militaris, in particular to a method for culturing Cordyceps militaris with high carotenoid yield, and more particularly to a method for liquid submerged fermentation and culturing of Cordyceps militaris with high yield of carotenoids. Background technique [0002] Cordyceps militaris (L.) Link, also known as Cordyceps militaris, belongs to Eumycota, Ascomycota, Ascomycotinia, Sordariomycetes, Hypomycetes Type species of Hypocreomycetidae, Hypocreales, Cordycipitaceae and Cordyceps. Cordyceps militaris usually has similar bioactive components with Cordyceps sinensis, mainly including cordycepin, cordycepin acid, cordyceps polysaccharide, ergosterol, amino acids, nucleosides, etc. The efficacy of Cordyceps militaris is also similar to that of Cordyceps sinensis, and it is a good substitute for Cordyceps sinensis. Cordyceps militaris is a natural resource, showing worldwide distribution, but the number is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/38C12P23/00C12R1/645
CPCC12N1/14C12N1/38C12P23/00
Inventor 邵颖陈安徽刘辉巫永华张爱文张建
Owner 安徽汉韵生物科技有限公司
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