Trichoderma harzianum solid fermentation method, solid fermentation product, trichoderma harzianum agent as well as preparation method and application of trichoderma harzianum agent

Abstract

The invention discloses a trichoderma harzianum solid fermentation method, a solid fermentation product, a trichoderma harzianum agent as well as a preparation method and an application of the trichoderma harzianum agent and relates to the technical field of microbial fermentation. According to the solid fermentation method, a solid fermentation tank is used for fermentation, and reproduction of trichoderma harzianum can be promoted, living contaminants can be reduced and the spore number of trichoderma harzianum can be increased by limiting humidity, turning and ventilation process parametersin the solid fermentation process. The trichoderma harzianum solid fermentation product prepared with the solid fermentation method is applied in the trichoderma harzianum agent, and the trichodermaharzianum agent can control grape downy mildew and grape gray mold efficiently and promote grape growth and increase of the yield of crops effectively and is a biological control preparation with great application prospects. Besides, the invention further provides the preparation method of the trichoderma harzianum agent. The method can fully utilize residues produced after solid fermentation, reduce emission of solid waste pollutants and save cost and is safe and environmentally friendly.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a solid fermentation method of Trichoderma harzianum, a solid fermentation product, a Trichoderma harzianum inoculant, and a preparation method and application thereof. Background technique [0002] According to the traditional classification system, Trichoderma harzianum belongs to anamorphic fungi, Hyphomycetes, Hyphomycetales, Hyphomycetaceae, Trichoderma sp. a member of Trichoderma is widely distributed and mainly exists in moist habitats such as forests, slopes and ditches, farmlands and grasslands. Plant residues such as soil, dead branches, fallen leaves and decayed wood, and fruiting bodies of other fungi are its growth substrates. Species are rich and diverse. Trichoderma is an important class of natural biological resources with high economic value. Among the Trichoderma genus, Trichoderma harzianum is the most widely used, and the inoculant made from ...

AI Technical Summary

Problems solved by technology

[0003] Most of the Trichoderma harzianum fungal agents commonly used in agriculture are spore preparations (live fungus preparations). The key to making Trichoderma harzianum fungal agents for agriculture is the number of spores contained in the fungal agent, but it is easy to appear during the production process of Trichoderma harzianum. The mycelium grows too vigorously, but the spore production is insufficient

The traditional fermentation methods of Trichoderma mainly include liquid fermentation and solid shallow plate fermentation. Liquid fermentation mainly produces chlamydospores, but the yield is extremely low, and the waste water and residue produced are easy to pollute the environment; solid shallow plate fermentation is semi-open, which is easy to cause miscellaneous Bacterial contamination, and occupies a large area, inconvenient to operate

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