Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Trichoderma harzianum solid fermentation method, solid fermentation product, trichoderma harzianum agent as well as preparation method and application of trichoderma harzianum agent

A technology of Trichoderma harzianum and solid fermentation, applied in the application, bactericide, biocide and other directions, can solve the problems of waste water and residue easily polluting the environment, excessive growth of mycelium, insufficient spore production, etc., to promote the growth of grapes, prevent The effect of plant disease is remarkable and the effect of reducing bacterial contamination

Active Publication Date: 2019-02-15
山东五福生生态工程有限公司
View PDF7 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Most of the Trichoderma harzianum fungal agents commonly used in agriculture are spore preparations (live fungus preparations). The key to making Trichoderma harzianum fungal agents for agriculture is the number of spores contained in the fungal agent, but it is easy to appear during the production process of Trichoderma harzianum. The mycelium grows too vigorously, but the spore production is insufficient
The traditional fermentation methods of Trichoderma mainly include liquid fermentation and solid shallow plate fermentation. Liquid fermentation mainly produces chlamydospores, but the yield is extremely low, and the waste water and residue produced are easy to pollute the environment; solid shallow plate fermentation is semi-open, which is easy to cause miscellaneous Bacterial contamination, and occupies a large area, inconvenient to operate

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Trichoderma harzianum solid fermentation method, solid fermentation product, trichoderma harzianum agent as well as preparation method and application of trichoderma harzianum agent
  • Trichoderma harzianum solid fermentation method, solid fermentation product, trichoderma harzianum agent as well as preparation method and application of trichoderma harzianum agent
  • Trichoderma harzianum solid fermentation method, solid fermentation product, trichoderma harzianum agent as well as preparation method and application of trichoderma harzianum agent

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0103] According to the fourth aspect of the present invention, a kind of preparation method of Trichoderma harzianum inoculant is provided, comprising the following steps: the solid fermentation product of Trichoderma harzianum is dried and separated to obtain Trichoderma harzianum spore powder, and then mixed with solid fermentation medium powder and carrier mixing to obtain the Trichoderma harzianum inoculant; the solid fermentation medium powder is obtained after the solid fermentation medium remaining after solid fermentation is crushed and separated.

[0104] Preferably, the ratio of Trichoderma harzianum spore powder: solid fermentation medium powder and the total amount of carrier is 1: (10-20).

[0105] Typical but non-limiting examples of Trichoderma harzianum spore powder: solid fermentation medium powder and carrier total ratio are 1:10, 1:12, 1:14, 1:16, 1:18 or 1:20.

[0106] It should be noted that the particle size of the solid fermentation product of Trichoder...

Embodiment 1

[0120] A kind of Trichoderma harzianum solid fermentation method, comprises the following steps:

[0121] (1) Seed activation: Trichoderma harzianum was inoculated on potato dextrose agar medium, and cultured at 25°C for 5 days to obtain activated Trichoderma harzianum seeds;

[0122] (2) Seed liquid preparation: inoculate the activated Trichoderma harzianum seeds in potato glucose liquid culture medium, cultivate at 28° C. for 24 hours, and the mycelium concentration reaches 180 g / L to obtain the Trichoderma harzianum seed liquid;

[0123] (3) Seed liquid culture: the Trichoderma harzianum seed liquid is inoculated in the liquid fermentation medium by 1.5‰ inoculum, cultivated in the seed tank, and obtains the Trichoderma harzianum seed liquid culture product;

[0124] The liquid fermentation medium comprises the following components in parts by weight: 1 part of corn flour, 2.5 parts of glucose, 2 parts of soybean flour cake, KH 2 PO 4 0.05 part, NaCl 0.2 part, (NH 4 ) ...

Embodiment 2

[0133] A kind of Trichoderma harzianum solid fermentation method, comprises the following steps:

[0134] (1) Seed activation: Trichoderma harzianum was inoculated on potato dextrose agar medium, and cultured at 28° C. for 3 days to obtain activated Trichoderma harzianum seeds;

[0135] (2) Seed liquid preparation: inoculate the activated Trichoderma harzianum seeds in potato glucose liquid medium, cultivate at 25° C. for 48 hours, and the mycelium concentration reaches 220 g / L to obtain the Trichoderma harzianum seed liquid;

[0136] (3) Seed liquid culture: the Trichoderma harzianum seed liquid is inoculated in the liquid fermentation medium by 2.5‰ inoculum, cultivated in the seed tank, and obtains the Trichoderma harzianum seed liquid culture product;

[0137] The liquid fermentation medium comprises the following components in parts by weight: 3 parts of corn flour, 1 part of glucose, 5 parts of soybean flour cake, KH 2 PO 4 0.04 part, NaCl 0.5 part, (NH 4 ) 2 SO 4 ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a trichoderma harzianum solid fermentation method, a solid fermentation product, a trichoderma harzianum agent as well as a preparation method and an application of the trichoderma harzianum agent and relates to the technical field of microbial fermentation. According to the solid fermentation method, a solid fermentation tank is used for fermentation, and reproduction of trichoderma harzianum can be promoted, living contaminants can be reduced and the spore number of trichoderma harzianum can be increased by limiting humidity, turning and ventilation process parametersin the solid fermentation process. The trichoderma harzianum solid fermentation product prepared with the solid fermentation method is applied in the trichoderma harzianum agent, and the trichodermaharzianum agent can control grape downy mildew and grape gray mold efficiently and promote grape growth and increase of the yield of crops effectively and is a biological control preparation with great application prospects. Besides, the invention further provides the preparation method of the trichoderma harzianum agent. The method can fully utilize residues produced after solid fermentation, reduce emission of solid waste pollutants and save cost and is safe and environmentally friendly.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a solid fermentation method of Trichoderma harzianum, a solid fermentation product, a Trichoderma harzianum inoculant, and a preparation method and application thereof. Background technique [0002] According to the traditional classification system, Trichoderma harzianum belongs to anamorphic fungi, Hyphomycetes, Hyphomycetales, Hyphomycetaceae, Trichoderma sp. a member of Trichoderma is widely distributed and mainly exists in moist habitats such as forests, slopes and ditches, farmlands and grasslands. Plant residues such as soil, dead branches, fallen leaves and decayed wood, and fruiting bodies of other fungi are its growth substrates. Species are rich and diverse. Trichoderma is an important class of natural biological resources with high economic value. Among the Trichoderma genus, Trichoderma harzianum is the most widely used, and the inoculant made from ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/14C12N3/00A01N63/04A01P3/00C12R1/885
CPCA01N63/30C12N1/14C12N3/00
Inventor 刘峰潘明王江伟郭红伟王珍伟
Owner 山东五福生生态工程有限公司
Features
  • Generate Ideas
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com