Efficient stevioside preparation method

A steviol glycoside and high-efficiency technology, which is applied in the field of high-efficiency steviol glycoside preparation, can solve problems such as low extraction rate, and achieve the effects of reducing production cost, improving product quality, and saving production cost and cycle.

Inactive Publication Date: 2019-02-19
山东奥晶生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] There are many traditional extraction processes of steviol glycosides in industrial production, including hot water extraction, dialysis, enzymatic, supercritical carbon dioxide extraction, ultra-high pressure, reflux sleeve extraction, countercurrent, etc., but these methods often There is a defect of low extraction rate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] After crushing the high-quality dry stevia leaves that were screened, pass through a 60-mesh sieve, soak the stevia powder with 2% concentration of acetic acid aqueous solution for 2 hours, filter the stevia filter residue, and then use 2% concentration of acetic acid aqueous solution to soak for 3.5 hours. Repeat the filtration 2 times after 1 hour, combine the filtrate obtained after 3 times of filtration, the filtrate is through 10000 molecular ultrafiltration, and then after 200 molecular nanofiltration, the specific gravity of the obtained sugar solution is 1.1, and the sugar solution is put into the resin chromatography column , and the volume ratio of the sugar solution to the chromatography column is 3:1, the sugar solution is controlled to be chromatographed at a flow rate twice the volume of the chromatography column for 1 hour, and the column is evenly washed with pure water after the sugar solution flows out, and the used The volume ratio of pure water to the...

Embodiment 2

[0031] After crushing the high-quality dry stevia leaves that were screened, pass through a 60-mesh sieve, and soak the stevia powder with 2% concentration of acetic acid aqueous solution for 4 hours, and then use 2% concentration of acetic acid aqueous solution to soak for 5 hours after filtering to get the stevia filter residue. Repeat the filtration 2 times after 1 hour, combine the filtrate obtained after 3 times of filtration, the filtrate is through 10000 molecular ultrafiltration, and then after 200 molecular nanofiltration, the specific gravity of the obtained sugar solution is 1.1, and the sugar solution is put into the resin chromatography column , and the volume ratio of the sugar solution to the chromatography column is 2:1, the sugar solution is controlled to be chromatographed at a flow rate twice the volume of the chromatography column for 1 hour, and the column is evenly washed with pure water after the sugar solution flows out, and the used The volume ratio of ...

Embodiment 3

[0033] After crushing the high-quality dry stevia leaves that were screened, pass through a 60-mesh sieve, soak the stevia powder with 2% acetic acid aqueous solution for 2 hours, filter to obtain the stevia filter residue, and then use 2% acetic acid aqueous solution to soak for 6 Repeat the filtration 2 times after 1 hour, combine the filtrate obtained after 3 times of filtration, the filtrate is through 10000 molecular ultrafiltration, and then after 200 molecular nanofiltration, the specific gravity of the obtained sugar solution is 1.1, and the sugar solution is put into the resin chromatography column , and the volume ratio of the sugar solution to the chromatography column is 2:1, the sugar solution is controlled to be chromatographed at a flow rate twice the volume of the chromatography column for 1 hour, and the column is evenly washed with pure water after the sugar solution flows out, and the used The volume ratio of pure water to the chromatography column is 1:1. Af...

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PUM

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Abstract

The invention relates to an efficient stevioside preparation method which includes the step: sequentially performing crushing, liquid glucose preparation, chromatography, column washing, nano-filtration solution preparation, desalination and spray drying on a certain weight of screened high-quality dry stevia rebaudiana leaves to prepare stevioside. The preparation method has the advantages that atraditional production process of the stevioside is changed, production cost and cycle are reduced, production progress is accelerated, safety of stevioside production is improved, use of ethyl alcohol serving as an inflammable and explosive article is omitted, product quality is improved, and environmental pollution caused by wastewater is reduced.

Description

technical field [0001] The invention relates to the technical field of stevioside extraction, in particular to a preparation method of high-efficiency stevioside. Background technique [0002] As a new type of natural sweetener, steviol glycoside has been favored by people and has been widely used. Steviol glycoside is an active ingredient in Stevia rebaudiana, which belongs to natural low-calorie high-intensity sweetener. It contains a variety of tetracyclic diterpene monomers with different components. These monomers all have the same aglycon-steviol (Steviol), which can be divided into stevioside (Stevioside), stevia Glycoside A (RebaudiosideA), B(RebaudiosideB), C(RebaudiosideC), D(Rebaudioside D), E(RebaudiosideE), DulcosideA, Steviolbioside and Rubusoside . Among these monomers, stevioside (Stevioside) and stevioside A (RebaudiosideA) account for 5%-9.5% and 2%-4.5% of the dry weight of the leaves respectively. The sweetness is 300 and 450 times that of sucrose, resp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/256C07H1/08
CPCC07H1/08C07H15/256
Inventor 郑越郑潇苏延营郑书平
Owner 山东奥晶生物科技有限公司
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