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Method for preparing oil-producing yeast fermentation medium from dehydrated sludge and application thereof

A fermentation medium and oleaginous yeast technology, which is applied in the field of microbial oil synthesis and sludge resource treatment, can solve the problems of not meeting the normal growth requirements of oleaginous yeast, low carbon-nitrogen ratio, and cultivating oleaginous yeast in medium , to achieve the effects of significant production promotion, low production cost, and realization of energy

Active Publication Date: 2019-02-19
TONGJI UNIV
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  • Description
  • Claims
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Problems solved by technology

The pretreatment method used requires the use of physical and chemical methods such as higher temperature or strong acid and strong alkali. These operations will produce some substances that are toxic or inhibitory to microorganisms.
In addition, the carbon-nitrogen ratio in the fermentation broth after anaerobic fermentation of dewatered sludge is very low, which cannot meet the normal growth needs of oleaginous yeast
Therefore, for high-concentration dewatered sludge, the acid-producing liquid obtained after pretreatment and anaerobic fermentation cannot be directly used as a medium for cultivating oleaginous yeast

Method used

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  • Method for preparing oil-producing yeast fermentation medium from dehydrated sludge and application thereof
  • Method for preparing oil-producing yeast fermentation medium from dehydrated sludge and application thereof
  • Method for preparing oil-producing yeast fermentation medium from dehydrated sludge and application thereof

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Embodiment

[0042] (1) Hot alkali pretreatment: add water to dilute the dewatered sludge to 3% to 5% VS, put it in a triangular conical flask, stir it evenly, adjust the pH to 10 with lime milk, and put it in a water bath at 90±5°C In the process, a vertical mixer was used to stir at a speed of 70-80rpm for 6 hours, and the bottle mouth was sealed during the hot alkali pretreatment process. After pretreatment, take out the pretreatment mixture, and centrifuge to obtain the pretreatment supernatant, the centrifugal force is 20000×g, and the centrifugation time is 20 minutes.

[0043] (2) Anaerobic fermentation: Centrifuge and concentrate the sludge in the concentration tank as the inoculum, the centrifugal force is 5000 × g, and the centrifugation time is 10min, inoculate the concentrated sludge to the pretreated clear liquid at a ratio of 10% v / v, and stir After uniformity, adjust the pH to 8 with 6M hydrochloric acid, and carry out anaerobic fermentation in an anaerobic fermentation tank...

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Abstract

The invention relates to a method for preparing an oil-producing yeast fermentation medium from dehydrated sludge and an application thereof. The method comprise the following steps: (1) using dehydrated sludge produced by a sewage treatment plant as a raw material, after dilution to medium concentration, obtain clean liquid by using hot alkali pretreatment; (2) inoculating the sludge in a concentration tank of the same sludge treatment plant to the clear liquid, and carrying out anaerobic fermentation at room temperature to obtain clear liquid; (3) removing the humic acid, protein and ammonianitrogen in the obtained solution by using a regulating method combing coagulation, acid extraction and guano precipitation to obtain and the solution with high C / N and high volatile fatty acid; (4)after sterilization, using the obtained solution as the oil-producing yeast fermentation medium; (5) culturing oil-producing yeast by using batch culture mode; (6) extracting microbial oil from the cultured yeast cells. The method achieves the resource utilization and energy utilization of the dehydrated sludge, and has certain environmental and economic benefits.

Description

technical field [0001] The invention relates to a method for preparing oleaginous yeast fermentation medium from dewatered sludge and an application thereof, which belongs to the field of sludge resource treatment and also relates to the field of microbial oil synthesis. Background technique [0002] Volatile fatty acids are carbon sources that can be directly utilized by yeast. Compared with the traditional carbon source glucose, the conversion pathway of volatile fatty acids to oil is shorter and the theoretical conversion efficiency is higher. Previous studies have used the volatile fatty acid solution obtained from anaerobic fermentation of excess sludge (water content 99%) as a carbon source to cultivate oleaginous yeast, and obtained microbial oil that can be used for the preparation of biodiesel. However, the excess sludge has a high moisture content and is not easy to transport and store. The dewatered sludge obtained by the sewage treatment plant after deep dehydra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C12P7/64C12R1/645
CPCC12N1/16C12P7/64
Inventor 刘佳黄翔峰穆天帅和文洁何拓李尚禹彭开铭陆丽君
Owner TONGJI UNIV
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