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Coffee senna tissue culture regeneration system construction method

A construction method and tissue culture technology, applied in culture medium, horticultural methods, plant regeneration, etc., can solve the problems of browning of explants, limitation of application of tissue culture technology, difficulty in rooting, etc., and achieve the effect of solving the shortage

Inactive Publication Date: 2019-02-26
钟天路
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, some progress has been made in the research on tissue culture in Wangjiang River, but there are still some problems, such as serious browning of explants, low shooting rate, difficulty in rooting, and low survival rate in transplanted field, which make tissue culture technology in the hope. Applications in Jiangnan are very limited

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] (1) Seed disinfection: select immature fruits, remove leaves, soak in washing powder water for 15 minutes, rinse with tap water for 2 hours after brushing, take out immature milky white or slightly brown seeds, and peel off the outer testa in an ultra-clean workbench , first sterilized with 75% ethanol for 6 seconds, then washed 6 times with sterile water, then sterilized with 0.1% mercuric chloride solution for 12 minutes, rinsed with sterile water 5 times and then set aside. The statistical pollution rate can be as low as 3% one week after inoculation.

[0014] (2) Embryo callus induction: the immature embryos treated in step (1) were cut into 0.5cm×0.5cm×0.5cm and inoculated on the induction medium, and cultured at 28°C in total darkness for 30 days. Then placed in light for 12 hours a day, the light intensity was 1400x, until embryogenic callus was induced, and the callus induction rate was 67%. The induction medium is ER+6-BA1.0mg / L+NAA0.5mg / L+GA 3 5 mg / L+sucrose ...

Embodiment 2

[0019] (1) Seed disinfection: select immature fruits, soak in washing powder water for 16 minutes after removing leaves, rinse with tap water for 3 hours after brushing, take out immature milky white or slightly brown seeds, and peel off the outer testa in an ultra-clean workbench , first sterilized with 75% ethanol for 8 seconds, then washed 9 times with sterile water, then sterilized with 0.1% mercuric acid solution for 11 minutes, rinsed with sterile water 9 times and then used for later use. The statistical pollution rate can be as low as 6% one week after inoculation.

[0020] (2) Embryo callus induction: the immature embryos treated in step (1) were cut into 0.5cm×0.5cm×0.5cm and inoculated on the induction medium, and cultured at 28°C in total darkness for 31 days. Then placed in light for 13 hours a day, the light intensity was 1400x, until embryogenic callus was induced, and the callus induction rate was 63%. The induction medium is ER+6-BA1.0mg / L+NAA0.2mg / L+GA 3 3 m...

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PUM

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Abstract

The invention discloses a coffee senna tissue culture regeneration system construction method. The coffee senna tissue culture regeneration system construction method adopts an asexual rapid reproduction technology to obtain coffee senna seedlings with stable plant performance. Coffee senna immature embryos are utilized as explants, and after steps such as seed sterilization, embryonic callus induction, differentiation culture, rooting culture, seedling hardening and transplanting, a coffee senna tissue culture regeneration system is constructed. By the adoption of the coffee senna tissue culture regeneration system construction method, not only is the problem of high-quality coffee senna seedling shortage solved, but also a foundation is laid for the development of genetic transformationof coffee senna in the future.

Description

technical field [0001] The invention relates to a method for plant tissue culture in agricultural biotechnology, in particular to a method for constructing a Wangjiangnan tissue culture regeneration system. Background technique [0002] Wangjiangnan is a leguminous plant, and its dry and mature seeds are taken. Mainly produced in Guangdong and Guangxi. The mature seeds are harvested in autumn and dried. The taste is slightly bitter. Has clearing away heat and improving eyesight, moistening intestines. For liver heat headache, dizziness, conjunctival congestion and swelling, constipation. Wangjiangnan plant tissue culture technology has the characteristics of accelerating the breeding process, shortening the breeding time, improving plant quality, saving space, reducing labor, and can be produced all year round without being restricted by natural conditions. It can effectively solve the problem of time-consuming breeding of Wangjiangnan varieties. In recent years, some p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G24/12A01G24/28A01H4/00
CPCA01G24/12A01G24/28A01H4/001A01H4/008
Inventor 钟天路
Owner 钟天路