Preparation method of in-situ cross-linked collagen fiber

Abstract

The invention discloses a preparation method of in-situ cross-linked collagen fiber. The preparation method comprises following steps: 1, EDC and NHS are mixed at a certain mass ratio, an obtained mixture is dissolved in 0.5mol / L glacial acetic acid as a crosslinking agent (wherein the mass amount of EDC accounts for 1 to 20% of that of collagen) so as to obtain a glacial acetic acid solution containing the crosslinking agent; 2, collagen is dissolved in the glacial acetic acid solution containing the crosslinking agent, mechanical stirring is adopted for full stirring, in dissolving process,the temperature of the solution is maintained at 4 to 6 DEG C, and at last a collagen solution with a mass concentration of 1.5% is prepared; 3, the collagen solution is subjected to freezing centrifugation defoaming for 40min at 4 DEG C so as to obtain a collagen spinning stock solution, wet method spinning is adopted, syringe pump extrusion speed is controlled to be 0.5ml / min, the stay time of obtained nascent fiber in a coagulation bath is controlled to be 100 to 300s so as to obtain collagen fiber, the gel state collagen fiber discharged from coagulation bath is loaded with 5g counterweight and is subjected to suspension treatment, and after 1h of natural airing, collagen fiber is obtained. According to the preparation method, introduction of EDC / NHS is capable of improving collagen fiber crystallization degree, in-situ crosslinking is capable of shortening the crosslinking time of collagen fiber, and improving forming technical conditions. The tensile strength of the collagen fiber prepared using the preparation method ranges from 1.06 to 1.43cN / dTex.

Description

technical field [0001] The invention relates to a method for preparing collagen fibers, in particular to a method for preparing collagen fibers which are cross-linked in situ by using EDC / NHS. Background technique [0002] Collagen, as an indispensable and water-insoluble fibrin outside the cells, is a structural protein that makes up the extracellular matrix (EMC) of organisms. Its appearance is generally white and transparent, without any branched fibrils. From the appearance, collagen macromolecules are in the shape of long and thin rods, which can be dissolved in strong acid or strong alkali solution, and have excellent water retention. Collagen, which provides elasticity and tension to cells, can undergo a series of reactions in the extracellular matrix to form semi-crystalline fibers, and play a key role in cell growth and migration. From the perspective of the microscopic structure of collagen, collagen molecules are composed of three α-peptide chains. The dimer pep...

AI Technical Summary

Problems solved by technology

However, if the collagen fibers cross-linked in the cross-linking bath want to achieve a higher degree of cross-linking, a longer cross-linking time is usually required to fully carry out the cross-linking reaction, which has certain limitations in industrial production and application.