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Broccoli protein-derived ACE (Angiotensin Converting Enzyme) inhibitory peptide as well as preparation method and application thereof

A protein source, broccoli technology, applied in the field of food and medicine, to achieve the effect of simple structure, wide application prospects, and inhibition of ACE activity

Active Publication Date: 2019-03-01
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few studies at home and abroad about the effective polypeptide extracts extracted from broccoli stems and leaves for the prevention and treatment of hypertension

Method used

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  • Broccoli protein-derived ACE (Angiotensin Converting Enzyme) inhibitory peptide as well as preparation method and application thereof
  • Broccoli protein-derived ACE (Angiotensin Converting Enzyme) inhibitory peptide as well as preparation method and application thereof
  • Broccoli protein-derived ACE (Angiotensin Converting Enzyme) inhibitory peptide as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] An ACE inhibitory peptide derived from broccoli protein, the amino acid sequence of the ACE inhibitory peptide is Ile-Pro-Pro-Ala-Tyr-Thr-Lys.

Embodiment 2

[0027] The preparation method of the ACE inhibitory peptide of broccoli protein source in the above-mentioned embodiment 1, its steps comprise:

[0028] 1. Extraction of protein from broccoli stems and leaves

[0029] Squeeze fresh broccoli stems and leaves as raw materials, heat the obtained juice at 90° C., keep it warm for 10 minutes, centrifuge the sediment, and dry to obtain the crude broccoli protein extract.

[0030] 2. Enzymatic preparation of polypeptide extract from broccoli stems and leaves and determination of polypeptide content

[0031] Using broccoli protein crude extract as raw material, add distilled water according to the mass ratio of solid to liquid 1:20, and add Aclase alkaline protease with 2.0wt% of the broccoli protein crude extract at a temperature of 60°C and pH 8.0, After enzymatic hydrolysis for 3 hours, the enzyme was inactivated, cooled, centrifuged, rotatively evaporated, concentrated and then freeze-dried to obtain the broccoli polypeptide extr...

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Abstract

The invention discloses a broccoli protein-derived ACE (Angiotensin Converting Enzyme) inhibitory peptide as well as a preparation method and application thereof. The broccoli protein-derived ACE inhibitory peptide is characterized in that an amino acid sequence of the ACE inhibitory peptide is Ile-Pro-Pro-Ala-Tyr-Thr-Lys; the preparation method of the broccoli protein-derived ACE inhibitory peptide comprises the following steps: (1) taking broccoli fresh stems and leaves as raw materials and squeezing; heating obtained juice to 90 DEG C; carrying out thermal insulation for 10 min; centrifuging a sediment and drying to obtain broccoli protein crude extract; (2) preparing broccoli polypeptide extract from polypeptide extract in broccoli stems and leaves through an enzymic method; (3) carrying out ultrafiltration and gel filtration on the broccoli polypeptide extract and collecting a component with the strongest ACE inhibitory activity; (4) carrying out high-performance liquid chromatographic separation and purification on the broccoli polypeptide extract and collecting the component with the strongest ACE inhibitory activity to obtain the broccoli protein-derived ACE inhibitory peptide; the broccoli protein-derived ACE inhibitory peptide can be used for preparing foods or medicines for assisting blood pressure lowering and has the advantages of simple structure, safety and strong activity.

Description

technical field [0001] The invention relates to the technical field of food and medicine, in particular to an ACE inhibitory peptide derived from broccoli protein and its preparation method and application. Background technique [0002] Broccoli is a vegetable crop with strong seasonality. The flower bulbs are harvested from November to March of the next year. The stems and leaves of the plants after the flower bulbs are harvested are piled up in the field as waste, which is seriously Pollution of the environment in the main broccoli producing areas. According to calculations, the yield of broccoli stems and leaves is generally 2.5-3 tons per mu. Only based on the 70,000-mu broccoli planting base in Shangpan Town, Linhai City, Zhejiang Province, the total amount can reach 175,000-210,000 tons. Excessive accumulation of broccoli stems and leaves seriously affects the virtuous cycle of the ecological environment of the soil plowing layer. The nitrogen and phosphorus elements ...

Claims

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Application Information

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IPC IPC(8): C07K14/415C07K1/36C07K1/34C07K1/16C12P21/06A23L33/18A61K38/01A61P9/12
CPCA23V2002/00A61K38/011A23L33/18A61P9/12C07K14/415C12P21/06A23V2200/326
Inventor 党亚丽潘道东高新昌曹锦轩周亭屹
Owner NINGBO UNIV
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