Unlock instant, AI-driven research and patent intelligence for your innovation.

Preparation method of double-fluorescence-labeled nano material

A nano-material and dual-fluorescence technology, applied in the field of fluorescent materials, can solve the problems of narrow excitation spectrum, overlapping peaks, easy photobleaching, etc., and achieve high sensitivity and good slow-release results

Active Publication Date: 2019-03-01
江西中洪博元生物技术有限公司
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional organic fluorescent labeling materials limit their application scope due to some of their own properties, such as: narrow excitation spectrum, broad emission spectrum, overlapping peaks caused by tailing, easy photobleaching and photolysis, etc.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of double-fluorescence-labeled nano material
  • Preparation method of double-fluorescence-labeled nano material
  • Preparation method of double-fluorescence-labeled nano material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0033] Specific embodiments of the present invention will be described in detail below.

[0034] A method for preparing dual fluorescent labeled nanomaterials includes the following steps:

[0035] (1) Dissolve HP-β-CD in DMSO, then add triethylamine, dissolve and mix thoroughly, and then set aside; dissolve CDI in DMSO, add it to the solution in the previous step after it is completely dissolved; then mix the liquid Stirring under the protection of nitrogen and avoiding light, add an equal volume of pre-cooled ether to the mixed liquid, mix to produce precipitation and filter, and then dissolve the filtered HP-β-CD-CDI sample in DMSO to form HP-β- DMSO solution of CD-CDI;

[0036] (2) First dissolve PEI in DMSO, then add triethylamine, after completely dissolving and mixing, add dropwise to the HP-β-CD-CDI solution under nitrogen protection and avoid light, and instill it slowly for a duration Over 2h, then stir overnight to obtain HP-β-CD-PEI solution;

[0037] (3) Put the HP-β-CD...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a preparation method of a double-fluorescence-labeled nano material. The preparation method comprises the following steps: connecting an intermediate substance CDI with HP-beta-CD; enabling HP-beta-CD-CDI and PEI to react in an organic solvent to prepare an HP-beta-CD-PEI solution; after dialysis, performing freeze-drying to obtain HP-beta-CD-PEI; under the catalysis of EDC(ethylene dichloride), coupling with rhodamine B to obtain an HP-beta-CD-PEI-RhB solution; after dialysis, performing freeze-drying to obtain HP-beta-CD-PEI-RhB; adsorbing fluorescein sodium throughthe HP-beta-CD-PEI-RhB; performing dialysis and freeze-drying to obtain the double-fluorescence-labeled nano material. The double-fluorescence-labeled nano material prepared by the method has the characteristics of non-radioactive performance, high stability, high sensitivity, high selectivity and the like; when being applied to researches of medicine activity, the double-fluorescence-labeled nanomaterial has no influence on the activity of medicines.

Description

Technical field [0001] The invention relates to the field of fluorescent materials, in particular to a method for preparing dual fluorescent labeling nano materials. Background technique [0002] Fluorescence is a common luminescence phenomenon in nature, that is, a phenomenon in which fluorescent compounds emit long-wavelength fluorescence after being irradiated with short-wavelength ultraviolet light. To be more precise, fluorescence refers to some fairly bright colors that human eyes can see under external light, such as green, orange, and yellow. People often call them neon lights. Fluorescent materials refer to materials that absorb light of a certain wavelength and immediately emit light of different wavelengths, called fluorescent materials. [0003] Fluorescence labeling technology refers to the use of some substances that emit fluorescence to covalently bond or physically adsorb on a certain group of the molecule to be studied, and use its fluorescence characteristics to ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C08G81/00C09K11/06B82Y20/00B82Y30/00B82Y40/00
CPCB82Y20/00B82Y30/00B82Y40/00C08G81/00C09K11/06C09K2211/14
Inventor 江斌刘贤武
Owner 江西中洪博元生物技术有限公司