Method for promoting micro algae frond to rapidly accumulate grease

A microalgae algae, rapid technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms and other directions, can solve problems such as unfavorable large-scale application production, low oil conversion rate, complex technical process, etc. The effect of large-scale application production, improving conversion rate and good application effect

Inactive Publication Date: 2019-03-01
HEXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the prior art, the use of microalgae to produce bio-oil usually requires large-scale professional facilities such as photobioreactors and fermentation tanks. The requirements for equipment are relatively high, the technical process is relatively complicated, and the conversion rate of oil is low and the cost is high, which is not conducive to large-scale production. Scale application production

Method used

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  • Method for promoting micro algae frond to rapidly accumulate grease

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Effect test

Embodiment 1

[0021] A method for promoting rapid accumulation of oil in microalgae, comprising the following steps:

[0022] (1) Preparation of algae liquid: under the conditions of temperature 23°C, light intensity 1850 lx, and light-dark ratio 12h / 12h, culture the microalgae to the logarithmic growth phase to obtain the algae liquid. The microalgae uses No. 9 freshwater pellets Algae;

[0023] (2) Hormone induction: brassinosterol is added to the 200ml algae liquid in step (1), until the final concentration of brassinosterol is 20.2mg / l;

[0024] (3) Obtain algae with high oil content: put the algae fluid induced by the hormone in step (2) in BG11 medium, cultivate it for 17 days under the conditions of temperature 24°C, light intensity 3900lx, and 24h continuous light, centrifuge and collect it. To obtain algae with high oil content, the centrifugation speed is 6000r / min, and the centrifugation time is 8min.

Embodiment 2

[0026] A method for promoting rapid accumulation of oil in microalgae, comprising the following steps:

[0027] (1) Preparation of algae liquid: culture the microalgae to the logarithmic growth phase under the conditions of temperature 24°C, light intensity 1900 lx, and light-dark ratio 12h / 12h, and obtain the algae liquid. The microalgae uses No. 31 freshwater pellets Algae;

[0028] (2) Hormone induction: brassinosterol is added to the 220ml algae liquid in step (1), until the final concentration of brassinosterol is 20.4mg / l;

[0029] (3) Obtain algae with high oil content: place the algae fluid induced by the hormone in step (2) in BG11 medium, culture it for 18 days under the conditions of temperature 25°C, light intensity 3950lx, and 24h continuous light, then centrifuge and collect it. To obtain algae with high oil content, the centrifugation speed is 6300r / min, and the centrifugation time is 7min.

Embodiment 3

[0031] A method for promoting rapid accumulation of oil in microalgae, comprising the following steps:

[0032] (1) Preparation of algae liquid: under the conditions of temperature 25°C, light intensity 1950lx, and light-to-dark ratio 12h / 12h, culture the microalgae to the logarithmic growth phase to obtain the algae liquid. The microalgae uses No. 9 freshwater pellets Algae;

[0033] (2) Hormone induction: brassinosterol is added to the 250ml algae liquid in step (1), until the final concentration of brassinosterol is 20.8mg / l;

[0034] (3) Obtain algae with high oil content: place the algae fluid induced by the hormone in step (2) in BG11 medium, culture it for 19 days at a temperature of 224°C, a light intensity of 4000 lx, and 24 hours of continuous light, then centrifuge and collect it. To obtain algae with high oil content, the centrifugation speed is 7000r / min, and the centrifugation time is 6min.

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Abstract

The invention relates to the field of micro algae energy, in particular to a method for promoting a micro algae frond to rapidly accumulate grease. The method comprises the following steps of 1, preparing an algae solution, wherein micro algae is cultured to the logarithm growth stage on the conditions that the temperature is 23-25 DEG C, the light intensity is 1,850-2,100 lx, the light and dark ratio is 12h / 12h, and the algae solution is obtained; 2, performing hormone induction, wherein brassinosteroid is added into 200-300 ml of algae solution in step 1 till the final concentration of the brassinosteroid is 20.2-21.5 mg / l; 3, obtaining the frond with the high grease content, wherein the algae solution obtained after hormone induction in step 2 is placed into a BG11 culture medium and cultured for 17-20 d on the condition that the temperature is 23-25 DEG C, the light intensity is 3,900-4,200 lx and light illumination is performed for 24 h continuously, and centrifuging and collecting are performed to obtain the frond with the high grease content; the method is easy to implement, free of contamination, high in efficiency, easy to realize and high in safety; in addition, the method can promote increase of grease content in micro algae, the grease conversion rate is greatly increased, and the method is beneficial to large-scale production and application.

Description

technical field [0001] The invention relates to the field of microalgae energy, in particular to a method for promoting the rapid accumulation of oil by microalgae. Background technique [0002] With the continuous consumption of fossil energy, the continuous emission of carbon dioxide and the intensification of the greenhouse effect, finding new sustainable renewable energy can effectively reduce the total emission of carbon dioxide in the atmosphere. Many renewable biomass have been used to produce biodiesel, including biomass raw materials such as rapeseed, jatropha, palm trees, food waste and microalgae, among which microalgae is a fast-growing and highly adaptable organism , has great nutritional, pharmaceutical and biological value, and can be used as a raw material in some biological and pharmaceutical industries to produce high value-added nutrients, biodiesel and fish bait. Compared with other biomass, microalgae is a kind of biomass with great development prospect...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12P7/64C12R1/89
CPCC12N1/12C12P7/6463
Inventor 杨生辉陈天仁王丹霞罗光宏
Owner HEXI UNIV
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