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A kind of extraction method of turtle peptide

An extraction method and turtle meat technology, which are applied in the field of natural peptide extraction, can solve the problems of immature turtle peptide production and extraction methods, complex process methods, and few active substances, and achieve a product with good taste, simple preparation process and good absorption. Effect

Active Publication Date: 2021-06-04
HEBEI ZIFENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Aiming at the problems that the existing turtle peptide production and extraction methods are immature, and the process method is complicated, the cycle is too long, the production cost is high, and the active substances are few, the present invention provides a turtle peptide extraction method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A method for extracting turtle peptide, comprising the following process steps:

[0025] a. After slaughtering the turtle, remove the shell and viscera, grind the turtle meat with a meat grinder, take 100g of the turtle meat, add purified water 7 times the mass of the turtle meat, and cook at 95°C for 7 hours; this process can destroy Cell structure, exposing the protein in the cell, and at the same time denature the protein in the soft-shelled turtle meat, which is conducive to the hydrolysis of protease.

[0026] b. Cool down to 57°C, add lipase accounting for 1% of the mass of soft-shelled turtle meat and glucoamylase accounting for 0.5% of the mass of soft-shelled turtle meat, and react for 1 hour under stirring conditions, wherein the lipase is fermented by Aspergillus niger, and the glucoamylase is obtained from Rhizopus oryzae Obtained by fermentation; this process can degrade fat and polysaccharides in soft-shelled turtle meat, so as to improve the yield and qua...

Embodiment 2

[0032] A method for extracting turtle peptide, comprising the following process steps:

[0033] a. After slaughtering the turtle, remove the shell and viscera, grind the turtle meat with a meat grinder, take 100g of the turtle meat, add purified water that is 8 times the mass of the turtle meat, and cook for 6 hours at 96°C; this process can destroy Cell structure, exposing the protein in the cell, and at the same time denature the protein in the soft-shelled turtle meat, which is conducive to the hydrolysis of protease.

[0034] b. Cool down to 56°C, add lipase accounting for 1.5% of the mass of soft-shelled turtle meat and glucoamylase accounting for 1% of the mass of soft-shelled turtle meat, and react for 1 hour under stirring conditions, wherein the lipase is fermented by Aspergillus niger, and the glucoamylase is obtained from Rhizopus oryzae Obtained by fermentation; this process can degrade fat and polysaccharides in soft-shelled turtle meat, so as to improve the yield...

Embodiment 3

[0040] A method for extracting turtle peptide, comprising the following process steps:

[0041] a. After slaughtering the turtle, remove the shell and viscera, grind the turtle meat with a meat grinder, take 100g of the turtle meat, add purified water that is 8 times the mass of the turtle meat, and cook for 6 hours at 98°C; this process can destroy Cell structure, exposing the protein in the cell, and at the same time denature the protein in the soft-shelled turtle meat, which is conducive to the hydrolysis of protease.

[0042] b. Cool down to 55°C, add lipase accounting for 1.5% of the mass of soft-shelled turtle meat and 2-glucoamylase accounting for 1% of the mass of soft-shelled turtle meat, and react for 0.5 h under stirring conditions, wherein the lipase is fermented by Aspergillus niger and the glucoamylase is obtained from rice Fermented by rhizopus; this process can degrade fat and polysaccharides in soft-shelled turtle meat, so as to improve the yield and quality of ...

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Abstract

The invention relates to the technical field of extracting natural peptides, and specifically discloses a method for extracting turtle peptides. The extraction method of the turtle peptide comprises the following process steps: a. mince the turtle meat, add water to cook; b. add lipase and glucoamylase to react; c. add calcium sulfate, then add bacillus protease and pancreatic protease respectively Protease, cysteine ​​protease and lactobacillus protease are reacted; d, the temperature is raised to inactivate the enzyme; e, separation and purification are carried out to obtain the turtle peptide. The Chinese soft-shelled turtle peptide product obtained by the present invention has high purity, and the separation amount of the peptide product is between 180-500 Daltons, so that the human body can better absorb it, and the absorption rate reaches 100%. At the same time, the method has a short production cycle, low cost, and It does not produce any toxic and harmful substances, is safe, has no toxic and side effects, and its products have a good taste and no peculiar smell, and can be widely used in food, health care products, medicines and other fields.

Description

technical field [0001] The invention relates to the technical field of extracting natural peptides, in particular to a method for extracting turtle peptides. Background technique [0002] Chinese soft-shelled turtle is a common farmed turtle species. Its meat is delicious and nutritious, and its protein content is as high as about 16%. The conventional processing methods of soft-shelled turtles are mainly steaming and simple enzymatic hydrolysis, which do not control the molecular weight, and the content of components with high biological activity is small, and the human body absorption rate is low. Therefore, how to extract a protein polypeptide from soft-shelled turtles that is easier for the human body to absorb and utilize, has low production costs, and has high biological activity is of great significance to human health. Contents of the invention [0003] Aiming at the problems that the existing turtle peptide production and extraction methods are immature, and the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06
CPCC12P21/06
Inventor 张晓东杨广杨子丰刘福兴
Owner HEBEI ZIFENG BIOTECH