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UPLC characteristic map construction method and detection method of ginger medicine

A technology of feature map and construction method, which is applied in the field of UPLC feature map construction of ginger medicinal materials, can solve problems such as it is difficult to fully reflect the quality characteristics of ginger, and achieve the effect of improving quality control level, good reproducibility and ensuring quality.

Active Publication Date: 2019-03-01
GUANGDONG YIFANG PHARMA
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The traditional method about the establishment of the fingerprint of ginger mostly adopts the HPLC method, and all of them are only aimed at the raw medicinal material of ginger itself, and mostly use fat-soluble components as index components, or use 6-gingerol, 8-gingerol and 10- As an index component, gingerol is mainly used to identify the authenticity, origin, and quality difference of ginger medicinal materials, and it is difficult to fully reflect the quality characteristics of ginger

Method used

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  • UPLC characteristic map construction method and detection method of ginger medicine
  • UPLC characteristic map construction method and detection method of ginger medicine
  • UPLC characteristic map construction method and detection method of ginger medicine

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Experimental program
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Embodiment 1

[0068] This embodiment is a method for constructing the UPLC characteristic map of ginger medicinal material.

[0069] 1. Instruments and raw materials

[0070] Instrument: ultra-high performance liquid chromatography (Vanquish, Thermo Fisher), Acclaim C18 column (2.1mm × 100mm, 2.2μm), Agilent SB C 18 Chromatographic column (100mm×2.1mm, 1.8μm), one-thousandth balance (ME204E, Mettler-Toledo Company), one-millionth balance (XP26, Mettler-Toledo Company), electric heating constant temperature water bath Pot (HWS-28, Shanghai Yiheng Technology Co., Ltd.).

[0071] Reagents: ethanol (Tianjin Fuyu Fine Chemical Co., Ltd.), methanol (analytical grade, Tianjin Fuyu Fine Chemical Co., Ltd.); 36% acetic acid (Tianjin Kemiou Chemical Reagent Co., Ltd.), acetonitrile (motive Ke Co., Ltd.), methanol (Merck Co., Ltd.) was HPLC chromatographic grade, and water was a laboratory ultrapure water system (Merck Co., Ltd., Milli-QDirect).

[0072] Test drug: 6-gingerol (batch number: 110885-...

Embodiment 2

[0220] Present embodiment is a kind of detection method of ginger medicinal material, and the steps are as follows:

[0221] 1. Chromatographic conditions

[0222] Stationary phase: Acclaim C 18 (2.2μm, 2.1x100mm); injection volume: reference substance solution 2μL, test solution 5μL; flow rate: 0.4mL / min; column temperature: 30°C; methanol: acetonitrile (volume ratio 4:1) as mobile phase A, using water as the mobile phase B, carry out gradient elution as specified in the table below; the detection wavelength is 280nm; the column temperature is 30°C; the flow rate is 0.4mL / min. The number of theoretical plates should not be less than 5000 based on the 6-gingerol peak.

[0223]

[0224] 2. Preparation of the sample solution: Take about 1 g of the ginger medicinal material to be tested (batch number: SJ19), weigh it accurately, put it in a conical flask, add 25 mL of methanol, weigh it, heat it under reflux for 30 minutes, let it cool, and weigh it again , Use methanol to ...

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Abstract

The invention relates to a UPLC characteristic map construction method and detection method of ginger medicine. The characteristic map construction method comprises the following steps of weighing 6-gingerol, 6-shogaol and 8-gingerol as reference substances, adding a solvent to dissolve the substances to obtain a reference solution; weighing the ginger medicine, adding an extraction solvent and heating for reflux extraction; then filtering and taking a filtrate as a test solution; taking a ginger standard decoction and preparing a standard decoction test solution by the same method; conductingultra-high performance liquid chromatography detection: drawing the reference solution, the test solution and the standard decoction test solution, injecting the solutions into a liquid chromatographrespectively and comparing an obtained test map and a standard decoction test map to determine the water-soluble common peak and obtain a UPLC characteristic map. The characteristic map can be used for qualitative and quantitative analysis of the quality of the ginger medicine, can ensure the quality of traditional ginger soup prepared by the medicine, and is also suitable for detecting other preparations containing the ginger.

Description

technical field [0001] The invention relates to quality research of Chinese medicinal materials, in particular to a construction method and detection method of UPLC characteristic map of ginger medicinal materials. Background technique [0002] Ginger (Zingiber officinale Rosc.), also known as ginger, belongs to the perennial herbaceous plant of Zingiberaceae Zingiberaceae. ... pick it up in September". It is slightly warm in nature and pungent in taste. It has the effects of relieving the exterior and dispelling cold, warming the middle to stop vomiting, resolving phlegm and relieving cough, and detoxifying fish and crabs. Mainly used for anemofrigid cold, stomach cold vomiting, cold phlegm cough, fish and crab poisoning. The chemical components of ginger mainly include gingerols, volatile oils, flavonoids, amino acids, sugars, organic acids and inorganic elements, among which gingerols and volatile oils are the main components, such as 6-gingerol and 8-gingerol , 10-gin...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/36
CPCG01N30/02G01N30/36
Inventor 朱德全彭劲源罗宇琴官永河陈万发霍文杰
Owner GUANGDONG YIFANG PHARMA
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