Preparation method for oxygen-sensitive multifunctional nonometer probe, and product thereof

An oxygen-sensitive, nano-probe technology, applied to medical preparations containing active ingredients, microcapsules, pharmaceutical formulas, etc., can solve problems such as failure to obtain a clear diagnosis and treatment plan, non-created imaging, etc., to meet production and application requirements , simple process and strong operability

Inactive Publication Date: 2019-03-08
SHANGHAI NAT ENG RES CENT FORNANOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the excellent performance of PLGA and the research progress of current tumor treatment drugs, the clinical treatment strategies for hypoxic lung cancer cells include fluorescent imaging molecular nanoprobes, PET functional molecular nanoprobes, MRI molecular nanoprobes and photothermal In response to photodynamic therapy, the development of these new functional molecular nanoprobes overcomes the disadvantages of traditional diagnosis and treatment methods, making non-inventive imaging possible, but the efficacy of hypoxia treatment is not as good as that of O 2 The quantitative relationship between concentrations still fails to obtain a clear diagnosis and treatment plan. The present invention aims to explore and develop a functional molecular nanoprobe for hypoxic tumor treatment, so as to realize the therapeutic effect on tumors and O 2 Quantitative monitoring of concentration

Method used

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  • Preparation method for oxygen-sensitive multifunctional nonometer probe, and product thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] An oxygen-sensitive multifunctional nanoprobe, based on oxygen-sensitive materials, is packaged and modified by biomolecules, and then compounded with photosensitive molecules and fluorocarbons to form an oxygen-sensitive multifunctional nanoprobe, as follows Steps to prepare:

[0024] Add 7.5 parts of 25 mg / mL NH2-PEG solution into the pyrene solution to prevent its agglomeration, and stir at room temperature for 12 hours under nitrogen protection atmosphere to obtain NH2-PEG-pyrene nanoparticles.

[0025] Dissolve 200 parts of 5 mg / mL TR780 in 2 parts of NH2-PEG-pyrene nanoparticles solution, then add 2 parts of pure ethanol and 1 part of FC-4.

[0026] Add 3.67 parts of 25% glutaraldehyde cross-linked NH2-PEG-MnO 2 After 24 hours, deionized water was used to remove impurities to obtain a nanoparticle solution, which was freeze-dried to form a powder. The SEM image of the obtained oxygen-sensitive multifunctional nanoprobe is shown in figure 1 .

Embodiment 2

[0028] An oxygen-sensitive multifunctional nanoprobe, the same as in Example 1, is prepared as follows:

[0029] Add 7.5 parts, 25mg / mL of BSA solution into [Cu(POP)(ptpm)]BF4 solution to prevent its agglomeration, and stir at room temperature under nitrogen protection atmosphere for 12h, to obtain BSA-[Cu(POP)(ptpm)]BF4 nanometer particle.

[0030] Dissolve 200 parts of 5 mg / mL BPD-MA in 4 parts of BSA-[Cu(POP)(ptpm)]BF4 nanoparticles solution, and then add 4 parts of pure ethanol.

[0031] Add 3.67 parts of 25% glutaraldehyde to cross-link the amine group of BSA-[Cu(POP)(ptpm)]BF4, remove impurities with deionized water after 24h to obtain a nanoparticle solution, and lyophilize to form a powder.

Embodiment 3

[0033] An oxygen-sensitive multifunctional nanoprobe, the same as in Example 1, is prepared as follows:

[0034] Add 7.5 parts of 25 mg / mL APTES solution to the coumarin solution to prevent its agglomeration, and stir at room temperature for 12 h under nitrogen protection atmosphere to obtain APTES-coumarin nanoparticles.

[0035] Dissolve 200 parts of 5 mg / mL HPD in 4 parts of APTES-coumarin nanoparticles solution, then add 4 parts of pure ethanol and 4 parts of FC-10.

[0036] Add 3.67 parts of 25% glutaraldehyde to cross-link the amino groups of APTES-coumarin, remove impurities with deionized water after 24 hours to obtain a nanoparticle solution, and freeze-dry to form a powder.

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Abstract

The invention relates to a preparation method for an oxygen-sensitive multifunctional nonometer probe, and a product thereof. The probe takes an oxygen-sensitive material as a basis, and the biocompatible oxygen-sensitive multifunctional nonometer probe is formed through the covering and the modification of biomolecules and through compounding with photosensitive molecules and fluorocarbon compound. Through the oxygen-sensitive multifunctional nonometer probe, a relationship between tissues and O2 concentration can be researched so as to be favorable for applying to the treatment of tumors ofanoxic microenvironment. The preparation method disclosed by the invention has the advantages of simple technology and high maneuverability, and production and application can be further met.

Description

technical field [0001] The invention relates to the field of preparation of nanomaterials, in particular to a preparation method of an oxygen-sensitive multifunctional nanoprobe and a product thereof. Background technique [0002] In recent years, pulmonary drug delivery has attracted more and more attention and research from researchers. Nanocarrier systems have many advantages in pulmonary drug delivery. If distributed in a relatively uniform dose of drug in the alveoli, the drug has a slow release effect, and a higher drug solubility can be achieved compared to its solubility in the aqueous phase. Precisely because of the many advantages of nanocarrier systems for pulmonary drug delivery, they are considered to be the best way to treat lung diseases. [0003] PLGA is a high molecular compound, and its safety in vivo is very good. It is extremely harmful to human health because its degradation products are metabolites of the human body and do not affect the normal physi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/22A61K41/00A61P35/00
CPCA61K41/0071A61K41/0076A61K49/225A61P35/00
Inventor 何丹农朱君李念念张心依刘睿金彩虹
Owner SHANGHAI NAT ENG RES CENT FORNANOTECH
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