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Application of Enterobacter hormaechei L124 to decomposition of agricultural wastes

A technology for Enterobacter horii and agricultural wastes, applied in the field of Enterobacter horii L124 and its degradation of agricultural wastes, can solve the problem of low enzyme activity, achieve rapid value-added, enzyme production, stable enzyme activity and adaptability strong effect

Active Publication Date: 2019-03-08
海林市中农国泰生物科技有限公司
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Problems solved by technology

However, the use of fungi to degrade lignocellulose generally has the problem of low enzyme activity

Method used

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  • Application of Enterobacter hormaechei L124 to decomposition of agricultural wastes
  • Application of Enterobacter hormaechei L124 to decomposition of agricultural wastes
  • Application of Enterobacter hormaechei L124 to decomposition of agricultural wastes

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specific Embodiment approach 1

[0025] Specific embodiment 1: A lignocellulosic substance degrading bacterium L124 of this embodiment, it is Enterobacter hormaechei (Enterobacter hormaechei) L124, is preserved in the general microorganism center of China Committee for Microorganism Culture Collection, and the preservation address is Beijing No. 3, Courtyard No. 1, Beichen West Road, Chaoyang District, the city, the date of preservation is December 12, 2014, and the preservation number is CGMCC No.10164.

[0026] Enterobacter hormaechei (Enterobacter hormaechei) L124 of the present embodiment is a Gram-negative bacterium, and the bacterial cell shape of the strain is rod-shaped, and the bacterial cell size is 0.55~0.58×1.4~1.5 μm, does not form spores, and has no flagella and pods Membrane; round, opaque, milky white colonies with smooth protrusions and neat edges formed on LB medium (such as figure 1 shown).

[0027] The results of the physiological and biochemical reactions of the strain included 18 items ...

specific Embodiment approach 2

[0086] Specific embodiment two: the application of a lignocellulosic substance degrading bacteria L124 in this embodiment, which is used to degrade agricultural waste, especially for degrading lignocellulosic substances in bagged fungus fertilizer and corn stalks .

[0087] Bacterial strain of the present invention is carried out following functional detection:

[0088] Enterobacter hormaechei (Enterobacter hormaechei) L124 of the specific embodiment was tested for weight loss rate and lignin, cellulose and hemicellulose degradation of bagged fungus waste and corn stalks to verify its unique functions. details as follows:

[0089] 1. Determination of weight loss rate of bagged fungus waste and corn stalks

[0090] 1.1 Corn stalk powder

[0091] Corn stalks were taken from the experimental field of the laboratory in Hulan Campus, Heilongjiang University, Harbin City, Heilongjiang Province in October 2012, dried, crushed and passed through a 40-mesh sieve, and set aside.

[...

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Abstract

The invention relates to Enterobacter hormaechei L124 and application thereof to decomposition of agricultural wastes. The Enterobacter hormaechei L124 is preserved in China General Microbiological Culture Collection Center, and the preservation number is CGMCC No. 10164. The Enterobacter hormaechei L124, provided by the invention, has the advantages of rapid bacterial strain proliferation, high adaptability and wide application; the weight loss ratio of bag planted edible fungus waste fermented by the Enterobacter hormaechei L124 is 30% or above; and the weight loss ratio of a maize straw fermented by the Enterobacter hormaechei L124 is 28% or above.

Description

[0001] This application is a divisional application of the following patent application, the application number of the original application: 201510861282.9, the application date: 2015-12-01, the name of the invention: a high-efficiency degrading lignocellulosic substance L124 and its application. technical field [0002] The invention relates to a strain of Enterobacter hallii L124 and its use for degrading agricultural waste. Background technique [0003] my country is a large agricultural country, and the amount of agricultural waste generated is extremely huge. Due to the limitations of economic benefits and technology popularization, most of the agricultural waste is extensively and inefficiently used and the state of idleness is serious, resulting in waste of resources and environmental pollution. Waste has become China's largest source of pollution. However, there is a large amount of lignocellulose in agricultural waste, which needs to be developed and utilized urgentl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09B3/00C12R1/01
CPCC12N1/205C12R2001/01
Inventor 杨峰山刘春光孙永帅吴奇丁仕波杨微王鲲鹏王庆华魏才强曲金玲张慧于文全
Owner 海林市中农国泰生物科技有限公司
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