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Method for preparing phosphate-solubilizing microorganism sustained-release sodium alginate microspheres by using acute hole method

A technology for phosphorus-resolving microorganisms and sodium alginate, which is applied in the field of preparing phosphorus-resolving microorganisms slow-release sodium alginate microspheres by a sharp hole method, can solve the problems of lack of good saprophytic ability, slow-release effect not durable enough, lack of competitiveness and the like , to achieve the effect of not easy to break, long duration, good slow release effect

Active Publication Date: 2019-03-08
WUHAN INSTITUTE OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Phosphorus-solubilizing microorganisms have been used in agricultural production for a long time, but the effect is not satisfactory. On the one hand, they lack strong competitiveness compared with local microorganisms. Major and other issues
In the prior art (such as CN106591278A, CN102476964A), although these problems can be solved to a certain extent by microsphere-coated slow-release, there are still deficiencies such as insufficient microsphere strength and insufficient sustained-release effect.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) Screening: Take 20g of soil from the phosphate mining area, mix it with 200mL of sterile water, stir it mechanically for 30 minutes, filter the resulting mixture with gauze and filter paper in turn, and collect the filtrate as the test bacteria solution.

[0025] Enrichment: Take 5 mL of the test bacteria solution and add it to 40 mL of NBRIP medium, and culture in a constant temperature shaker (cultivation temperature 30°C, rotation speed 165 rpm) for 3 days. Take 5mL of the bacterial solution after the first enrichment and repeat the enrichment 3 times in the same way to obtain the microbial flora in the phosphate mining area with phosphorus-dissolving effect.

[0026] Acclimatization: Take 5mL of the logarithmic phase of the above-mentioned microbial flora in the phosphate rock mining area that has the effect of dissolving phosphorus, and add it to 40mL of acclimatization medium for acclimatization and cultivation for 3 days. Take 5 mL of the first acclimatizatio...

Embodiment 2

[0032] (1) Screening: Take 20 g of farmland soil, mix it with 200 mL of sterile water, and stir it mechanically for 30 minutes, then filter the resulting mixture with gauze and filter paper in turn, and collect the filtrate as the test bacterial solution.

[0033] Enrichment: Take 5mL of the test bacteria solution and add it to 40mL NBRIP medium, and culture it in a constant temperature shaker (cultivation temperature 28°C, rotation speed 160rpm) for 4 days. Take 5mL of the bacterial solution after the first enrichment and repeat the enrichment 3 times in the same way to obtain the farmland soil microbial flora with phosphorus-solubilizing effect.

[0034] Acclimatization: Take 5mL of the above-mentioned farmland soil microbial flora with phosphorus-dissolving effect in the logarithmic phase, and add it to 40mL of acclimatization medium for acclimatization and cultivation for 3 days. Take 5 mL of the bacterial liquid obtained from the first domestication culture and add it to ...

Embodiment 3

[0039] (1) Screening: Take 20g of soil from the phosphate mining area, mix it with 200mL of sterile water, and stir it mechanically for 30 minutes, then filter the mixture with gauze and filter paper in turn, and collect the filtrate as the test bacteria solution.

[0040] Enrichment: Take 5 mL of the test bacterial solution and add it to 40 mL of NBRIP medium, and culture in a constant temperature shaker (cultivation temperature 30° C., rotation speed 170 rpm) for 3 days. Take 5mL of the bacterial solution after the first enrichment and repeat the enrichment 3 times in the same way to obtain the microbial flora in the phosphate mining area with phosphorus-dissolving effect.

[0041] Acclimatization: Take 5mL of the logarithmic phase of the above-mentioned microbial flora in the phosphate rock mining area that has the effect of dissolving phosphorus, and add it to 40mL of acclimatization medium for acclimatization and cultivation for 3 days. Take 5mL of the bacterial liquid af...

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Abstract

The invention relates to a method for preparing phosphate-solubilizing microorganism sustained-release sodium alginate microspheres by using an acute hole method. The method includes, firstly, screening, enriching and domesticating to obtain a target phosphorus-solubilizing microbial flora; then mixing the target phosphorus-solubilizing microbial flora with a sodium alginate solution with a certain concentration to obtain a mixed bacterial solution; adding a proper amount of hole-forming agents into the mixed bacterial solution; dripping the mixed bacterial solution into a CaCl2 solution through pinholes with different hole diameters to form the microspheres; and finally, optionally, dispersing the obtained microspheres into a chitosan solution again to obtain the secondary-coated microbial sustained-release sodium alginate microspheres. The microspheres are smaller in particle size and more regular in shape, double coating makes the microspheres have better strength and less easy to crack; and the microspheres have the advantages of good slow release effect, long duration and the like. As the coating materials used are sodium alginate and chitosan which have good biocompatibilityand can be naturally degraded, so that pollution and damage to soil can not be caused.

Description

technical field [0001] The invention relates to the technical field of slow-release materials and microorganisms, in particular to a method for preparing slow-release sodium alginate microspheres for phosphorus-dissolving microorganisms by using an orifice method. Background technique [0002] Sodium alginate is a natural heteropolysaccharide extracted from brown algae. Its unique structural characteristics make it often used as a matrix to encapsulate or deliver various active ingredients and biological agents. As a micro-storage, microspheres can be used to protect and store certain substances, and release the active ingredients through the diffusion and penetration of the capsule membrane. This mechanism is the main way for microspheres to function. Sustained-release microspheres are microsphere preparations made by embedding functional molecules or bacteria with special materials, which can slowly release the embedded substances under certain conditions, or become a micr...

Claims

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Application Information

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IPC IPC(8): C12N11/10C12N11/04C12N1/36C05F11/08
CPCC12N1/36C12N11/04C12N11/10C05F11/08
Inventor 肖春桥池汝安徐广
Owner WUHAN INSTITUTE OF TECHNOLOGY
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