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Method for preparing tilapia mossambica skin immunomodulating peptide

An immune regulation and tilapia technology, which is applied in the preparation method of peptides, chemical instruments and methods, peptides, etc., can solve the problems of heavy metals in immune regulation peptides and endanger human health, so as to improve complexation and removal effect , increase the effect of the removal effect

Active Publication Date: 2019-03-08
湖北健肽生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention aims at overcoming the problem in the prior art that the immunomodulatory peptide extracted from fish skin contains heavy metals that endanger human health, and provides a method for preparing an immunomodulatory peptide from tilapia fish skin. The immunomodulatory peptide does not contain heavy metal mercury, Chromium, nickel, zinc and other harmful substances

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The preparation method of adsorbent comprises the following steps:

[0025] Crush the crab shells to obtain crab shell powder, add the crab shell powder and dopamine into water at a mass ratio of 1:0.35, the mass volume ratio of the crab shell powder and water is 1:35g / mL, raise the temperature to 50°C, and then add pentadiene The molar ratio of aldehyde, glutaraldehyde and dopamine is 1:1.5, stirred for 1.5h, and the stirring rate is controlled at 100r / min, after filtering and washing, the crab shell powder is dried at 60°C for 2h to obtain pretreated crab shell powder , soak the pretreated crab shell powder in 8% acetic acid aqueous solution for 3 hours, wash with water until neutral, and dry, and then heat up the dried crab shell at 3.5°C / min under the purging of inert gas Raise the temperature to 320°C, roast for 40 minutes, then raise the temperature to 620°C at a heating rate of 7°C / min, and roast for 2 hours to obtain porous crab shell powder; add the porous crab...

Embodiment 2

[0032] The preparation method of adsorbent comprises the following steps:

[0033]Crush the crab shells to obtain crab shell powder, add the crab shell powder and dopamine into water at a mass ratio of 1:0.3, the mass volume ratio of the crab shell powder and water is 1:32g / mL, raise the temperature to 48°C, and then add pentamethylene glycol Aldehyde, the molar ratio of glutaraldehyde and dopamine is 1:1, stirring reaction for 1.5h, the stirring speed is controlled at 85r / min, after filtering and washing, the crab shell powder is dried at 55°C for 1.5h to obtain pretreated crab shell Powder, soak the pretreated crab shell powder in 6% acetic acid aqueous solution for 3 hours, wash with water until neutral, and dry the dried crab shell under the purging of inert gas at 3°C / min. The heating rate was raised to 310°C, roasted for 35 minutes, then raised to 610°C at a heating rate of 6°C / min, and roasted for 1.5 hours to obtain porous crab shell powder; the porous crab shell powde...

Embodiment 3

[0040] The preparation method of adsorbent comprises the following steps:

[0041] Crush the crab shells to obtain crab shell powder, add the crab shell powder and dopamine into water at a mass ratio of 1:0.45, the mass volume ratio of the crab shell powder and water is 1:38g / mL, raise the temperature to 55°C, and then add pentadiene The molar ratio of aldehyde, glutaraldehyde and dopamine is 1:1.8, stirred for 2 hours, and the stirring rate is controlled at 110r / min. After filtering and washing, the crab shell powder is dried at 65°C for 3 hours to obtain pretreated crab shell powder. Soak the pretreated crab shell powder in an 8% acetic acid aqueous solution for 4 hours, wash with water until neutral, and dry the dried crab shell under the purging of an inert gas at a heating rate of 3.5°C / min. Raise the temperature to 340°C, roast for 45 minutes, then raise the temperature to 630°C at a heating rate of 7°C / min, and roast for 2 hours to obtain porous crab shell powder; add t...

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PUM

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Abstract

The invention relates to the technical field of preparation of immunomodulating peptides and discloses a method for preparing a tilapia mossambica skin immunomodulating peptide for solving the problemthat human health is damaged by heavy metals contained in the fish skin. The method for preparing the immunomodulating peptide comprises the following steps: cleaning the skin, and removing heavy metals and impurity particles in the skin by using an adsorbent; degreasing the skin with absolute ethyl alcohol, removing impure proteins in the skin by utilizing sodium hydroxide, performing enzymolysis on the skin by using papain, filtering, and performing freeze drying, thereby obtaining the immunomodulating peptide. According to the method disclosed by the invention, the skin is adsorbed in advance, and the heavy metals in the skin are removed, so that the prepared immunomodulating peptide does not contain any heavy metal damaging the human health.

Description

technical field [0001] The invention relates to the technical field of preparation of immunomodulatory peptides, in particular to a preparation method of tilapia skin immunomodulatory peptides. Background technique [0002] The immune system is crucial to our survival and has the function of defending against pathogens, but the immune system is vulnerable to many factors, including stress, unhealthy living habits, pathogens and antigens, etc. Several drugs that have been found to regulate the human immune response include cyclosporine, tacrolimus, glucocorticoids, phytol, aristolochic acid, plumbagin, and levamisole. However, toxic side effects and high cost of these drugs limit their use in patients, and most immunomodulatory drugs are not suitable for chronic or preventive use. Studies have shown that oligopeptides, polypeptides, and some protease hydrolyzate mixed peptides obtained by commercial protease hydrolysis and separation and purification using food-derived prote...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/34C07K1/14
CPCC07K1/14C07K1/34C12P21/06
Inventor 余方苗董晓泽何康唐云平杨最素丁国芳
Owner 湖北健肽生物科技有限公司
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