Method for extracting tea polyphenols from camellia osmantha

A technology of tea polyphenols and camellia oleifera, which is applied in the field of natural product chemistry, can solve problems affecting product purity and achieve the effects of accelerated dissolution, low production cost, and efficient and accurate separation

Active Publication Date: 2019-03-12
GUANGXI FORESTRY RES INST
View PDF6 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the addition of metal ion complex precipitation in this extraction process, the added metal ion impurities will affect the purity of the product

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The method for extracting tea polyphenols from camellia oleifera comprises the following steps:

[0029] (1) Raw material pretreatment: collect the branches and leaves of Camellia oleifera, wash them with clear water, and after drying, sterilize them with an ultraviolet sterilizing lamp for 10 minutes, and crush them to 10 meshes to obtain raw materials for extraction;

[0030] (2) Enzymolysis: the extraction raw material is added in the composite enzyme aqueous solution (the mass ratio of the extraction raw material and the composite enzyme aqueous solution is 1:2, the mass fraction of the composite enzyme aqueous solution is 20%, and the composite enzyme is composed of pectinase and cellulase by mass ratio 1:2), adjust the pH value to 3.8 with acetic acid / sodium acetate buffer solution, heat up to 50°C for enzymolysis for 1 hour, heat up to 90°C to inactivate the enzyme after the enzymolysis is completed, and collect the enzymatic solution;

[0031] (3) Wall-breaking ...

Embodiment 2

[0037] The method for extracting tea polyphenols from camellia oleifera comprises the following steps:

[0038] (1) Raw material pretreatment: collect the branches and leaves of Camellia oleifera, wash them with clean water, sterilize them with an ultraviolet sterilizing lamp for 20 minutes after drying, and crush them to 30 meshes to obtain raw materials for extraction;

[0039] (2) Enzymolysis: the extraction material is added in the composite enzyme aqueous solution (the mass ratio of the extraction raw material and the composite enzyme aqueous solution is 1:4, the mass fraction of the composite enzyme aqueous solution is 22%, and the composite enzyme is composed of pectinase and cellulase according to mass ratio 1:2.5), adjust the pH value to 4.2 with acetic acid / sodium acetate buffer solution, heat up to 55°C for enzymolysis for 2 hours, heat up to 95°C to inactivate the enzyme after the enzymolysis is completed, and collect the enzymatic solution;

[0040] (3) Wall-break...

Embodiment 3

[0046] The method for extracting tea polyphenols from camellia oleifera comprises the following steps:

[0047] (1) Raw material pretreatment: collect the branches and leaves of Camellia oleifera, wash them with clear water, and after drying, sterilize them with an ultraviolet sterilizing lamp for 12 minutes, and crush them to 20 meshes to obtain the extracted raw materials;

[0048](2) Enzymolysis: the extraction material is added in the composite enzyme aqueous solution (the mass ratio of the extraction raw material and the composite enzyme aqueous solution is 1:4, the mass fraction of the composite enzyme aqueous solution is 22%, and the composite enzyme is composed of pectinase and cellulase according to mass ratio 1:2), adjust the pH value to 4.0 with acetic acid / sodium acetate buffer solution, heat up to 52°C for enzymolysis for 2 hours, heat up to 95°C after enzymolysis to inactivate the enzyme, and collect the enzymatic solution;

[0049] (3) Wall-breaking extraction: ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for extracting tea polyphenols from camellia osmantha. The method comprises the following steps of washing and drying the branches and leaves of the camellia osmantha,crushing, then performing enzymolysis and wall breaking extraction, filtering extract liquid roughly by adopting a 100-200-mesh filter screen, and then performing ultra-filtration to separate the teapolyphenols by adopting a 5-8 kDa ultrafiltration membrane according to the characteristics of molecular sizes of the tea polyphenols. Specific to the characteristics that the camellia osmantha has ahigh tea polyphenol content and a special lamina structure, the invention improves the extraction process of the tea polyphenols, and provides a low-cost high-yield method for extracting the tea polyphenols from the camellia osmantha, so that the utilization rate of planted products such as the camellia osmantha is improved, and the extraction cost of the tea polyphenols is lowered. In the tea polyphenol concentrate prepared by adopting the method disclosed by the invention, the tea polyphenols account for 98% or more of total solute, and the extraction rate of the tea polyphenols in extraction raw materials can be up to 70% or more.

Description

technical field [0001] The invention belongs to the technical field of natural product chemistry, and in particular relates to a method for extracting tea polyphenols from camellia oleifera. Background technique [0002] The planting area of ​​camellia oleifera forests in Guangxi is about 6 million mu, ranking third in the country. It is one of the main producing areas of camellia oleifera in the country. In the process of pruning and pruning of camellia oleifera trees and transformation of low-yield forests every year, a large number of leaves are often used as waste Abandoned, a great waste of resources. As an oil plant, after oil extraction every year, the quantity of remaining camellia oleifera husks is also very large. If it can be effectively used, it will generate huge economic benefits. At the end of 2011, a species of Camellia oleifera was discovered in Nanning, Guangxi. After the study of morphological characteristics and phenological differences, ISSR molecular i...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C07G99/00
CPCC07G99/00
Inventor 陈海燕杨漓梁忠云李桂珍叶海芹温如斯
Owner GUANGXI FORESTRY RES INST
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap