Functionalized mesoporous silicon-based tumor targeted transport and controlled release system and preparation method thereof
A tumor-targeting, mesoporous silicon technology, which is applied in the fields of anti-tumor drugs, capsule delivery, and pharmaceutical formulations, can solve the problems of inaccessible tumor site enrichment, difficulty penetrating tumor blood vessels, and large drug carrier size. Improve the effect of small drug loading
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Embodiment 1
[0056] 1. Preparation of thiol-functionalized mesoporous silicon nanoparticles (MSN-SH)
[0057] Accurately weigh 0.8g NaOH and add it to 480mL deionized water, then add 1.0g cetyltrimethylammonium bromide (CTAB) and dissolve it ultrasonically, stir vigorously and heat up to 80°C, 5.0mL tetraethyl orthosilicate ( TEOS) and 0.97 mL of 3-mercaptopropyltrimethoxysilane (MPTMS) were slowly added dropwise to the above solution, and the reaction was vigorously stirred at 80° C. for 2 h. After cooling to room temperature, the white solid was collected by centrifugation (9500r / min×8min), and ultrasonically cleaned several times with methanol and deionized water respectively, and the product was freeze-dried to obtain mesoporous silicon nanoparticles (CTAB @MSN-SH).
[0058] In order to remove the surfactant of mesoporous silicon nanoparticles, the extraction method under acidic conditions with methanol was used. Weigh 1.0g of CATB@MSN-SH and add it to 160mL of anhydrous methanol. Af...
Embodiment 2
[0076] 1. Preparation of thiol-functionalized mesoporous silicon nanoparticles (MSN-SH)
[0077] Accurately weigh 0.8g NaOH and add it to 480mL deionized water, then add 1.0g cetyltrimethylammonium bromide (CTAB) and dissolve it ultrasonically, stir vigorously and heat up to 80°C, 5.0mL tetraethyl orthosilicate ( TEOS) and 0.97 mL of 3-mercaptopropyltrimethoxysilane (MPTMS) were slowly added dropwise to the above solution, and the reaction was vigorously stirred at 80° C. for 2 h. After cooling to room temperature, the white solid was collected by centrifugation (9500r / min×8min), and ultrasonically cleaned several times with methanol and deionized water respectively, and the product was freeze-dried to obtain mesoporous silicon nanoparticles (CTAB @MSN-SH).
[0078] In order to remove the surfactant of mesoporous silicon nanoparticles, the extraction method under acidic conditions with methanol was used. Weigh 1.0g of CATB@MSN-SH and add it to 160mL of anhydrous methanol. Af...
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