Slurry for 3D printing, 3D structure as well as preparation method and application thereof

A 3D printing and structure technology, applied in prosthesis, medical science, additive processing, etc., can solve the problems of insufficient stability and lack of biological activity of the scaffold, and achieve the goal of improving biological inertness, promoting cell activity and function, and improving structure. The effect of block phenomenon

Active Publication Date: 2019-03-15
SHENZHEN INST OF ADVANCED TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the sodium alginate scaffold still uses calcium ions for cross-linking, and its stability is still insufficient.
To solve the problem of the stability of sodium alginate scaffolds, the researchers grafted methacrylic acid groups on the carboxyl groups of sodium alginate, and used photocrosslinking instead of calcium ion crosslinking, thereby improving the stability of sodium alginate scaffolds. Scaffolds lack bioactivity

Method used

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  • Slurry for 3D printing, 3D structure as well as preparation method and application thereof
  • Slurry for 3D printing, 3D structure as well as preparation method and application thereof
  • Slurry for 3D printing, 3D structure as well as preparation method and application thereof

Examples

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preparation example Construction

[0080] According to a second aspect of the present invention, a method for preparing a slurry for 3D printing is provided, comprising the following steps:

[0081] Mix alginate, polylysine and water evenly to obtain a slurry for 3D printing.

[0082] The preparation method of the slurry is simple and easy to obtain.

[0083] Preferably, the method for preparing the slurry for 3D printing includes the following steps: adding alginate into the solution of polylysine and water, and mixing evenly to obtain the slurry.

[0084] Specifically, the method includes the following steps: dissolving polylysine in a formula amount in water and mixing uniformly; adding alginate into the polylysine aqueous solution and mixing uniformly to obtain a 3D printing slurry.

[0085] Preferably, the solution of polylysine and water also includes effective biological ingredients.

[0086] Effective biological ingredients include one or more of nutrients, extracellular matrix, growth factors, cells ...

Embodiment 1

[0136] Example 1 Preparation of sodium alginate / polylysine bioink and its three-dimensional bioprinted bone model

[0137] A preparation method of sodium alginate / polylysine bioink and three-dimensional bioprinted bone model thereof, comprising the following steps:

[0138] (1) Dissolve 2.924g polylysine in 10mL double-distilled water and stir evenly, weigh 4g sodium alginate and add it to the polylysine solution and stir until uniform to obtain the carboxyl group of sodium alginate and polylysine The molar ratio of amino groups is sodium alginate / polylysine bioink of 1:1;

[0139] (2) Put the bio-ink into the printing barrel, and use the three-dimensional bio-printer to print the bone model, the printing pressure is 600kPa, and the printing speed is 10mm / s.

Embodiment 2

[0141] The difference between this example and Example 1 is that the molar ratio of the carboxyl group of sodium alginate to the amino group of polylysine is 2:1.

[0142] figure 2 For the bone model photo that embodiment 1 and embodiment 2 slurry print out, figure 2 It can be seen that the sodium alginate / polylysine bioink has self-supporting ability, can realize the printing of complex models, and has good formability.

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Abstract

The invention discloses slurry for 3D printing, a 3D structure as well as a preparation method and application thereof, and relates to the technical field of three-dimensional biological printing. Theslurry for 3D printing comprises alginate, polylysine and water, wherein the mass of alginate is 20-40% of the mass of water, and the molar ratio of carboxyl groups of alginate to amino groups of polylysine is 1:(0.1-2). The preparation method of the 3D structure provided by the invention comprises performing 3D printing using the slurry for 3D printing to obtain the 3D structure. The slurry provided by the invention has good formability, can be used for directly printing out a structure which can stably exist under a physiological environment, improves biological inertness of alginate at thesame time, can regulate and control the overall charge of a bracket by controlling the proportion of sodium alginate and polylysine, and is favorable for carrying different types of effective biological components to promote cell adhesion and proliferation. A printed structure has better stability after further chemical crosslinking and can maintain its integrity for a long time.

Description

technical field [0001] The invention relates to the technical field of three-dimensional bioprinting, in particular to a slurry for 3D printing, a 3D structure, and a preparation method and application thereof. Background technique [0002] Three-dimensional (3D, 3Dimensions) bioprinting technology is derived on the basis of three-dimensional printing technology. The printing technology of loading cells or growth factors while printing biological materials has great potential in the preparation of artificial organs and tissues and drug screening models. potential. Bio-inks (materials used for 3D bioprinting) determine the physical and chemical properties of 3D bioprinted products, and at the same time affect the survival rate of cells and the activity of growth factors. Alginate (sodium) is widely used in the construction of cell capsules and bio-inks because of its shear-thinning properties (the viscosity of the ink is reduced by shear force), as well as its excellent bioc...

Claims

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Application Information

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IPC IPC(8): A61L27/20A61L27/18A61L27/54A61L27/50B33Y10/00B33Y70/00B33Y80/00
CPCA61L27/18A61L27/20A61L27/50A61L27/54B33Y10/00B33Y70/00B33Y80/00C08L5/04C08L77/04
Inventor 阮长顺林子锋吴明明潘浩波
Owner SHENZHEN INST OF ADVANCED TECH
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