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A kind of preparation method of decellularized porcine cornea and its decellularized lamellar cornea and usage

A lamellar cornea and decellularization technology, which is applied in the field of preparation of decellularized porcine cornea, can solve the problems of corneal transparency, corneal stroma layer damage, animal-derived virus infection, etc., to overcome excessive drying and shorten enzyme treatment time , Guarantee the effect of decellularization effect

Active Publication Date: 2021-11-26
XIAMEN DAKAI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its defects: first, it cannot achieve complete decellularization effect; second, the enzymes listed above, especially protease, also have obvious damage to the extracellular matrix of the cornea, resulting in a decrease in the transparency of the cornea
[0015] It is not difficult to prove the fact that in the process of artificial cornea preparation, any processing link will inevitably cause damage to the regular arrangement in the corneal stroma, and thus cause extremely adverse effects on the "transparency" of the prepared cornea. influences
Although it can effectively solve the problems of immune rejection of organs for allograft transplantation and animal-derived virus infection, the different degrees of damage to the transparency of the cornea during the preparation process will also affect the transplantation effect to a large extent.

Method used

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  • A kind of preparation method of decellularized porcine cornea and its decellularized lamellar cornea and usage
  • A kind of preparation method of decellularized porcine cornea and its decellularized lamellar cornea and usage
  • A kind of preparation method of decellularized porcine cornea and its decellularized lamellar cornea and usage

Examples

Experimental program
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Embodiment 1

[0064] Such as figure 1 As shown, the preparation method of porcine acellular lamellar cornea provided in Example 1 consists of at least four processing processes. in:

[0065] Such as Figure 1A Shown, the first step S1 is to carry out pretreatment to porcine cornea, as Figure 1A shown. Specifically in this embodiment, the pretreatment should at least include the following processes: S1.1 Take fresh porcine cornea, remove the epithelial layer after cleaning; S1.2 Prepare lamellar cornea; the lamellar cornea only includes Bowman's membrane and stroma layer; S1.3 is cleaned; the pretreatment of fresh porcine cornea in the present invention is mainly to complete the preparation of lamellar cornea, and perform necessary cleaning in order to prepare for subsequent processing procedures, so the pretreatment of the present invention see S1.1~S1. Each processing method in 3 can adopt any conventional processing method.

[0066] In an optional implementation in the step S1.2 of...

Embodiment 2

[0089] Such as figure 2 As shown, the preparation method of porcine decellularized lamellar cornea provided in Example 2 has at least five treatment processes. in:

[0090] The degree of pretreatment in the first step S1 and the drying treatment in the second step S2 are approximately the same as those of S1 and S2 in Example 1, wherein the specific implementation method can be selected within the range listed in Example 1. Therefore, the first step S1 of pretreatment and the second step S2 of drying treatment in this embodiment 2 will not be described in detail.

[0091] S3: Decellularization treatment, decellularization treatment process of the dried cornea: S3.1 Enzyme treatment: use DMEM medium to configure totipotent nuclease solution; add 0.5-3 ml of the above enzyme solution to each piece of cornea; first vortex and shake until the air bubbles on the surface of the cornea are removed, and the gas in the cornea is removed; then place in a shaking incubator and shake ...

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Abstract

A preparation method of porcine acellular lamellar cornea and porcine acellular lamellar cornea. The preparation method is: pretreatment (S1): pretreatment of fresh porcine cornea to produce lamellar cornea; drying treatment (S2): drying the pretreated After the treatment, the lamellar cornea is dried; the enzyme treatment method is used for decellularization (S3): add the dried cornea to the enzyme solution; place the cornea in a shaking incubator for shaking treatment; then place the cornea in a shaking incubator for shaking and washing treatment, and obtain Decellularized cornea. Sterilization treatment: (S4).

Description

[0001] Field [0002] The invention relates to a preparation method of decellularized porcine cornea, and lamellar decellularized cornea made of porcine cornea which can be directly used in human keratoplasty and a use method thereof. Background technique [0003] Corneal blindness is the second leading cause of blindness in my country, and corneal transplantation is the only effective treatment for corneal blindness. However, the lack of corneal donor materials has seriously affected corneal transplantation. After more than ten years of hard work by Chinese scientists, the artificial cornea made of pig cornea can replace human cornea, and it has achieved the success of clinical trials for the first time in the world. [0004] The existing research results prove that the artificial cornea prepared from pig cornea has good biocompatibility. In particular, porcine cornea has a highly similar tissue structure, biophysical properties and optical properties to the human cornea, a...

Claims

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Application Information

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IPC IPC(8): A61L27/38
CPCA61L27/38
Inventor 李志寒李洁詹晓亮其他发明人请求不公开姓名
Owner XIAMEN DAKAI BIOTECH CO LTD