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Method of enriching daidzein with soybean sprouts

A technology of daidzein and bean sprouts, which is applied in the directions of seed immunity, seed and rhizome treatment, application, etc., can solve the problems of long enrichment period, high production cost, environmental pollution, etc., so as to shorten the enrichment period and avoid content The effect of reducing, enhancing the ability to resist stress

Inactive Publication Date: 2019-03-19
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The present invention is to provide a production process of soybean sprouts rich in daidzein, which overcomes the deficiencies of the prior art, can solve the problems of complex operation, high production cost, long enrichment cycle, and environmental pollution, and obtain a nutritional value High quality, good quality control, simple operation, low cost, new functional health-care soybean sprouts with high daidzein content

Method used

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  • Method of enriching daidzein with soybean sprouts
  • Method of enriching daidzein with soybean sprouts
  • Method of enriching daidzein with soybean sprouts

Examples

Experimental program
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Effect test

Embodiment 1

[0023] Take vigorous soybean seeds as raw materials, disinfect them with 1% (v / v) sodium hypochlorite solution, and then place them in an 8-fold (v / w) hydrogen peroxide solution with a concentration of 0.5 mmol / L. After soaking for 8 hours at 25℃, put the seeds in a germination tray and germinate for 4 days at 25℃. UV-B intermittent irradiation (wavelength is 298nm, irradiation intensity is 5μW·cm -2 , The irradiation time is 3h / day), during which the soybean sprouts are sprayed with hydrogen-rich water solution every 4h for 0.5min, the spray liquid flow rate is 50mL / min, the hydrogen-rich water solution is made by passing hydrogen into purified water, and the concentration is 0.05mmol / L. The content of daidzein in soybean sprouts is about 139μg / gDW.

Embodiment 2

[0025] Soybean seed selection and disinfection are the same as in Example 1. Then put it in 8 times (v / w) hydrogen peroxide solution with a concentration of 1mmol / L, soak at 25℃ for 8h, put the seeds in a germination tray, germinate at 25℃ for 3 days, UV-B Intermittent irradiation (wavelength is 303nm, irradiation intensity is 20μWcm -2 , The irradiation time is 6h / day), during which the soybean sprouts are sprayed with a hydrogen-rich water solution every 4h for 1 min. The preparation method of the hydrogen-rich water solution and the spray liquid flow rate are the same as those in Example 1, and the concentration is 0.1mmol / L. The content of daidzein in soybean sprouts is about 198μg / g DW.

Embodiment 3

[0027] Soybean seed selection and disinfection are the same as in Example 1. Then place 8 times (v / w) of hydrogen peroxide solution with a concentration of 9mmol / L, soak at 25℃ for 8h, put the seeds in a germination tray, germinate at 25℃ for 2 days, UV-B Intermittent irradiation (wavelength is 308nm, irradiation intensity is 20μWcm -2 , The irradiation time is 9h / day), during which the soybean sprouts are sprayed with a hydrogen-rich water solution every 4h for 1 min. The preparation method of the hydrogen-rich water solution and the spray liquid flow rate are the same as those in Example 1, and the concentration is 0.1mmol / L. The content of daidzein in soybean sprouts is about 165μg / g DW.

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Abstract

The invention relates to a method of enriching daidzein with soybean sprouts and belongs to the technical field of food production. The method is characterized in that physiologically active bean seeds are used as a raw material, the raw material is disinfected with sodium hypochlorite, the disinfected material is soaked in hydrogen perioxide solution, the soaked material is paced in a sprouting plate of a medium-wave ultraviolet (UV-B) radiation device, and a hydrogen-rich solution is sprayed to the material under UV-B intermittent radiation to allow sprouting; the content of daidzein in soybean sprouts reaches 139-400 mu g / g DW. The method herein has the advantages of good operational convenience, short production period, low cost, good safety and zero pollution, and large-scale production of soybean sprouts with rich daidzein is facilitated.

Description

1. Technical Field [0001] The invention relates to a method for enriching daidzein in soybean sprouts, and belongs to the technical field of food production. 2. Background technology [0002] Soy seeds are rich in nutrients such as protein, fat, and carbohydrates, as well as functional substances such as soybean isoflavones and gamma-aminobutyric acid. A total of 12 soy isoflavone isomers isolated from soybeans are divided into free aglycones and bound glycosides, of which aglycones only account for 2%-3% of the total isoflavones, including genistein and daidzein And three kinds of glycitein. The pharmacological activity of soybean isoflavones is mainly derived from free aglycone, and the antioxidant activity and biological function of daidzein are higher. After the soybean seeds germinate, the key enzymes in the synthesis of daidzein are activated, thereby enriching daidzein. The biotransformation method can further increase the content of daidzein to expand its application r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01C1/00A01C1/08
CPCA01C1/00A01C1/08
Inventor 顾振新马萌杨润强王沛
Owner NANJING AGRICULTURAL UNIVERSITY
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